ABSTRACT
The ability to produce biofilm and the presence of metallo-ß-lactamase (MBL) among Pseudomonas aeruginosa isolates were evaluated. A total of 91 isolates were recovered from sputa of patients with (CF, n = 44) and without (non-CF, n = 47) cystic fibrosis diagnosis. Seventy-nine (86.8%; 95% CI 78.3-92.3%) were biofilm producers. Interestingly, all isolates harboring MBL showed ability (most strong or moderate) to produce biofilm in vitro. We alert to an "overlapping of mechanisms" that together represent an even greater challenge for the treatment of pulmonary infections by P. aeruginosa.
Subject(s)
Biofilms/growth & development , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/physiology , beta-Lactam Resistance , beta-Lactams/pharmacology , Cystic Fibrosis/complications , Humans , Pseudomonas Infections/microbiology , Pseudomonas aeruginosa/isolation & purification , Sputum/microbiologyABSTRACT
Cystic fibrosis (CF) patients typically suffer of persistent and recurrent lung infections caused by Pseudomonas aeruginosa that many times possess ability for the biofilm production. Here, biofilm production among P. aeruginosa isolates recovered from sputum of CF and non-CF patients was evaluated. Most isolates were biofilm-producing independently of the patient's condition.
ABSTRACT
Cystic fibrosis (CF) patients typically suffer of persistent and recurrent lung infections caused by Pseudomonas aeruginosa that many times possess ability for the biofilm production. Here, biofilm production among P. aeruginosa isolates recovered from sputum of CF and non-CF patients was evaluated. Most isolates were biofilm-producing independently of the patient's condition.
ABSTRACT
Cystic fibrosis (CF) patients typically suffer of persistent and recurrent lung infections caused by Pseudomonas aeruginosa that many times possess ability for the biofilm production. Here, biofilm production among P. aeruginosa isolates recovered from sputum of CF and non-CF patients was evaluated. Most isolates were biofilm-producing independently of the patient's condition.
ABSTRACT
Members of the genus Staphylococcus are among the most important human pathogens, and strains demonstrating resistance to methicillin are an increasing problem worldwide, both within and outside of hospital environments. The objective of this study was to evaluate the use of variations of agar screening tests with cefoxitin and oxacillin to detect methicillin resistance in staphylococcal isolates. The agar screening test with cefoxitin (4 microg/ml) showed 99.4% accuracy for detecting both S. aureus and coagulase-negative staphylococci. The performance of the agar screening test with cefoxitin (4 microg/ml) either equaled or was superior to the other agar screening test variations evaluated and can be used to characterize the presence of the mecA gene among staphylococcal species.