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Sci Rep ; 5: 11130, 2015 Jun 09.
Article in English | MEDLINE | ID: mdl-26057350

ABSTRACT

HIV subtypes or clades differentially induce HIV-associated neurocognitive disorders (HAND) and substance abuse is known to accelerate HIV disease progression. The HIV-1 envelope protein gp120 plays a major role in binding and budding in the central nervous system (CNS) and impacts dopaminergic functions. However, the mechanisms utilized by HIV-1 clades to exert differential effects and the methamphetamine (METH)-associated dopaminergic dysfunction are poorly understood. We hypothesized that clade B and C gp120 structural sequences, modeling based analysis, dopaminergic effect, and METH potentiate neuronal toxicity in astrocytes. We evaluated the effect of clade B and C gp120 and/or METH on the DRD-2, DAT, CaMKs and CREBP transcription. Both the structural sequence and modeling studies demonstrated that clade B gp120 in V1-V4, α -2 and N-glycosylated sites are distinct from clade C gp120. The distinct structure and sequence variation of clade B gp120 differentially impact DRD-2, DAT, CaMK II and CaMK IV mRNA, protein and intracellular expression compared to clade C gp120. However, CREB transcription is upregulated by both clade B and C gp120, and METH co-treatment potentiated these effects. In conclusion, distinct structural sequences of HIV-1 clade B and C gp120 differentially regulate the dopaminergic pathway and METH potentiates neurotoxicity.


Subject(s)
HIV Envelope Protein gp120/metabolism , HIV-1/classification , Methamphetamine/metabolism , Neurons/drug effects , Receptors, Dopamine D2/metabolism , Amino Acid Sequence , Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism , Calcium-Calmodulin-Dependent Protein Kinase Type 4/metabolism , Cells, Cultured , Dopamine Agonists/pharmacology , Enzyme Inhibitors/pharmacology , Gene Expression , HIV Envelope Protein gp120/chemistry , Humans , Methamphetamine/toxicity , Molecular Sequence Data , Receptors, Dopamine D2/genetics , Sequence Homology, Amino Acid
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