Transcriptome analysis reveals involvement of oxidative stress response in a copper-tolerant Fusarium oxysporum strain.

High amount of copper is toxic to most organisms, but endophytic fungi can develop survival strategies to tolerate and respond to environmental stressors such as heavy metal contaminants. While high copper induces oxidative stress, it is still unclear which genes are associated with copper tolerance. Here, we performed a metatranscriptome analysis of endophytic fungi isolated from a black nightshade plant Solanum nigrum L. growing on mine tailings of a gold processing area. Initial screening revealed the presence of a copper-tolerant strain of Fusarium oxysporum, designated as IB-SN1W, which tolerated up to 1000 ppm and 300 ppm copper in solid and liquid media, respectively. Differential gene expression analysis by RNA sequencing showed that 23% of contigs are uniquely expressed in the copper-treated fungus. These genes are involved in copper ion import, polyamine transport, oxidoreductase activity, and oxidative stress response. Catalase transcripts were also highly upregulated in IB-SN1W compared to a non-tolerant F. oxysporum strain. Catalase inhibition decreased copper-tolerance in IB-SN1W, while the addition of antioxidants prevented the copper-dependent growth inhibition in the non-tolerant strain. Overall, these results suggest that oxidative stress response contributes to copper tolerance in F. oxysporum.

Assessment of Alternanthera sessilis and Aster philippinensis as excluders in a small-scale Cu-Au processing site at Kias, Benguet, Philippines.

In unregulated mining and processing for Cu and Au, large amounts of heavy metals and metalloids are generated as tails. These wasted by-products could actually pose serious environmental problems. The objective of this study was to assess the potential ability of Alternanthera sessilis and Aster philippinensis thriving abundantly in a small-scale mine processing site at Kias, Benguet, for possible Cu, Pb, Zn, and As uptake. It also aimed to determine the cellular localization of the contaminants within the plant biomass. Alternanthera sessilis and Aster philippinensis exhibited low bioaccumulation factor (BF) and translocation factor (TF) values for Cu, Pb, Zn, and As. The BF and TF values could suggest possible exclusion mechanisms of the plants in avoiding phytotoxicity. SEM-EDX analysis of the Alternanthera sessilis roots indicated higher weight % of Cu, Pb, and As in the epidermis, and Zn in the cortex. On the other hand, Aster philippinensis roots showed high weight % of Zn and As in the epidermis and Cu and Pb in the cortex. The localization of the contaminants in the root epidermal and cortical cells signifies restriction of their mobility to the xylem, preventing migration to the shoot system. The findings of this study suggest that Alternanthera sessilis and Aster philippinensis are considered potential phytostabilizers capable of immobilizing contaminant toxicity in the soil and in the rhizosphere.

Localization of nickel in the hyperaccumulator plant Breynia cernua (Poir.) Mull.Arg. discovered in the nickeliferous laterites of Zambales, the Philippines.

A newly discovered nickel (Ni) hyperaccumulator plant, Breynia cernua (Phyllanthaceae) from the nickeliferous laterites of Acoje, Zambales, has elevated Ni concentrations. Its computed bioaccumulation factor is 1.7 and the translocation factor is 4.6. Leaf tissues which contain the highest Ni concentrations were analyzed using optical microscopy and Scanning Electron Microscopy with Energy-Dispersive X-ray (SEM/EDX). The results indicated that most of the Ni are stored in the epidermal cells of the leaves, followed by the mesophyll cells then the vascular bundles. The accumulation of Ni found in the subcellular locations, probably in the vacuoles, may prevent phytotoxicity that could affect the normal function of other cellular structures. A possible resistance mechanism through strain avoidance towards Ni accumulation makes B. cernua capable of tolerating elevated Ni concentrations in its tissues. The tolerance of B. cernua to Ni phytotoxicity suggests possible applications of this hyperaccumulator plant in phytoextraction technology.

Pteris melanocaulon Fée is an As hyperaccumulator.

Pteris melanocaulon is noted to thrive very well in selected Cu-Au mines in the Philippines such as the Carmen Cu mine in Cebu and the Manila Mining Cu-Au mine in Surigao. Soil and plant samples were collected from field surveys as well as potted experiments. The computed bioaccumulation factor (BF) and translocation factor (TF) values from the analyses of As contents of the soil and the belowground and above ground components of the plant, apparently were comparable to known As hyperaccumulators Pteris vittata and Pityrogramma calomelanos. It is inferred with probability that the factors that influence the bioavailability of As as well as the uptake mechanisms for P. melanocaulon would be similar to the 2 known As hyperaccumulators. Previous works have noted P. melanocaulon as a very good Cu accumulator with very high Cu values in the belowground components and this study further identified it to be an excellent As hyperaccumulator with high As values in the aboveground components.

Towards integrated management of a shallow tropical lake: assessment of water quality, sediment geochemistry, and phytoplankton diversity in Lake Palakpakin, Philippines.

The limited carrying capacities of shallow tropical lakes render them more vulnerable to ecological problems like eutrophication. Unregulated human activities such as unsustainable aquaculture and urbanization can alter ecosystem dynamics rapidly, and this warrants more comprehensive researches than what has been previously conducted. Here, we presented an integrated assessment of the nutrient dynamics, phytoplankton diversity, and sediment geochemistry in Lake Palakpakin, a shallow tropical lake of volcanic origin, to understand its deteriorating ecological state. Water, phytoplankton, and sediment samples were collected, and in situ water quality measurements were done during wet and dry seasons in four critical areas in the lake, namely, the inlet, center, sanctuary, and outlet. Results revealed that high light extinction coefficient (1.13 m-1), high turbidity (28 NTU), high phosphate concentration (> 2.0.5 mg/L), and the abundance of Microcystis aeruginosa, Anabaena helicoidea, and Lyngbya sp. indicate that from a relatively healthy lake in 2008, Lake Palakpakin has become a eutrophic to hypereutrophic freshwater body. High concentrations of available nutrients such as N and P were detected in the center and sanctuary sediments, which drive the internal nutrient loading in the lake. We recommend that management efforts be directed towards a whole-ecosystem approach in addressing the problem of eutrophication, especially in shallow tropical lakes.

The identification of indigenous Cu and As metallophytes in the Lepanto Cu-Au Mine, Luzon, Philippines.

The mining activities in the Lepanto Cu-Au Mine which is situated within the Mankayan Mineral District in the Philippines have exposed the arsenic (As)-rich copper (Cu)-gold (Au) and polymetallic ores to surface conditions. Cu and As dispersal into nearby soils and waters could pose health hazards to the natural ecosystems and human settlements. The study focused on the identification of indigenous metallophytes thriving in the area as well as the bioavailability of Cu and As in soils and its implication to the growth of the indigenous plants. Particular interests were on plant species that are capable of Cu and As absorption and have potential applications to mine rehabilitation. The samples were analyzed for total Cu and As contents. The soil samples were also subjected to different physicochemical analyses such as pH, organic matter, and nutrient content. Fern species had relatively high Cu and As contents in their biomass than other plant species found in the study area. The Cu and As concentrations in the plants might have been strongly influenced by the bioavailability of the metal and metalloid which were dependent on the physicochemical properties of the soil such as pH, organic matter, and nutrient contents. These identified metallophytes namely Dicranopteris linearis, Histiopteris incisa, Pityrogramma calomelanos, Pteris vittata, Nephrolepis hirsutula, Pteris sp., Pinus sp., Thysanolaena latifolia, and Melastoma malabathricum have tolerated the different Cu and As concentrations in the soil thus could be useful and effective for ecological restoration as an option to post-mining rehabilitation.

Trace organic chemical pollutants from the lake waters of San Pablo City, Philippines by targeted and non-targeted analysis.

More than half of the freshwater lakes in the Philippines are small with surface areas of <2 km2. The dynamics in these lakes are different from those in the bigger lakes. This study was conducted to determine the organic pollutants and their sources in three of the seven lakes of San Pablo City in Laguna, Philippines: lakes Palakpakin, Sampaloc, and Pandin. Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography - Tandem Mass Spectrometry (LC-MS/MS) were used in the targeted and non-targeted analysis of the lake water samples. The three lakes are all volcanic crater lakes but are exposed to different anthropogenic activities, which includes domestic activities, livelihood (farming and aquaculture) and eco-tourism. Due to the presence of rice fields and fruit plantations, chlorpyrifos was detected in the three lakes while other pesticides like cypermethrin, picolinafen and quinoxyfen were additionally found in Lake Sampaloc, which is the biggest of the three lakes and located within the urbanized section of the city. Traces of different surfactants (linear alkylbenzene sulfonates, secondary alkyl sulfonates, alkyl sulfates, alkyl ether sulfates), biocide benzalkonium chloride, insect repellent diethyltoluamide, antibiotics (sulfadiazine and sulfamethoxazole), hypertension drug telmisartan, phosphate-based fire retardants, and artificial sweeteners (acesulfame, cyclamate, saccharin and sucralose) were detected in lakes Sampaloc and Palakpakin. The same surfactants, artificial sweeteners, insect repellant and phosphate-based fire retardants were also found in Lake Pandin, which is mainly used for eco-tourism activities like swimming and boating. The results of this study suggest that the organic pollutants present in the small lakes can be linked to the various human activities in the immediate lake environment. Because small lakes are more prone to environmental stresses, human activities in the said lakes must be regulated to ensure sustainable development.

Copper uptake by Pteris melanocaulon Fée from a Copper-Gold mine in Surigao del Norte, Philippines.

The ability of some plants to take up metal contaminants in the soil has been of increasing interest as an environmental approach to pollution clean-up. This study aimed to assess the ability of Pteris melanocaulon for copper(Cu) uptake by determining the Cu levels in the fern vis-à-vis surrounding soil and the location of Cu accumulation within its biomass. It also aimed to add information to existing literature as P. melanocaulon are found to be less documented compared to other fern metal accumulators, such as P. vittata. The P. melanocaulon found in the Suyoc Pit of a Copper-Gold mine in Placer, Surigao del Norte, Philippines exhibited a high Bioaccumulation Factor(BF) of 4.04 and a low Translocation Factor(TF) of 0.01, suggesting more Cu accumulation in the roots (4590.22 ± 385.66 µg g(-1) Cu). Noteworthy was the Cu concentration in the rhizome which was also high (3539.44 ± 1696.35 µg g(-1) Cu). SEM/EDX analyses of the Cu content in the roots indicated high elemental %Cu in the xylem (6.95%) than in the cortex (2.68%). The high Cu content in the roots and rhizomes and the localization of Cu in the xylem manifested a potential utilization of the fern as a metallophyte for rhizofiltration and phytostabilization.