Evaluation of Plasma Disappearance Rate Indocyanine Green Clearance as a Predictor of Liver Graft Rejection in Donor Brain Death.

INTRODUCTION: There currently exist no quantitative methods to assess graft viability before the donor procurement procedure. In Europe, around 20% of liver grafts evaluated "in situ" by an experienced surgeon are discarded. The aim of this study is to evaluate the use of the plasma disappearance rate indocyanine green (PDR-ICG) clearance in predicting liver graft rejection to avoid this 20% of futile surgeries. OBJECTIVES: To evaluate PDR-ICG as a predictor of liver graft rejection in death brain donors compared with the gold standard evaluation by an experienced surgeon. MATERIAL AND METHODS: Prospective observational single center study. From March 2017 to July 2019, 29 donors were included in the study, 17 were men and 12 women with a median age of 68 years ± 16.9 years. Donors had an intensive care unit stay of 2 days ± 4 days. PDR-ICG was measured with PICCO2 monitor. Indocyanine green clearance dose was 0.25 mg/kg injected intravenously in the operating room just before donor procurement procedure is initiated. The surgeon was unaware of the PDR-ICG measure until the decision of graft acceptance was taken. Data regarding the donors and biopsy results were included in a prospective database. RESULTS: PDR-ICG measure could be obtained in 10 minutes in all of the cases included. The median PDR-ICG obtained was 18%/min (range, 2.4-31%/min). Graft rejection took place in 15 out of the 29 donors. PDR-ICG value was less than 10%/min in 6 of these rejected grafts and less than 15%/min in 10 donors. All donor grafts with PDR-ICG <15% were discarded. The graft had been discarded in 5 donors with a PDR-ICG >15%. CONCLUSIONS: In our study a plasma disappearance rate <10 would have identified the grafts that would be rejected, thus avoiding the displacement work and expense of the surgical team. These results should be confirmed in a multicentric study.

Donor Risk Index Has an Impact in Intraoperative Measure of Hepatic Artery Flow and in Clearance of Indocyanine Green: An Observational Cohort Study.

BACKGROUND: The increase in indications for liver transplantation has led to acceptance of donors with expanded criteria. The donor risk index (DRI) was validated with the aim of being a predictive model of graft survival based on donor characteristics. Intraoperative arterial hepatic flow and indocyanine green clearance (plasma clearance rate of indocyanine green [ICG-PDR]) are easily measurable variables in the intraoperative period that may be influenced by graft quality. Our aim was to analyze the influence of DRI on intraoperative liver hemodynamic alterations and on intraoperative dynamic liver function testing (ICG-PDR). METHODS: This investigation was an observational study of a single-center cohort (n = 228) with prospective data collection and retrospective data analysis. Measurement of intraoperative flow was made with a VeriQ flowmeter based on measurement of transit time (MFTT). The ICG-PDR was obtained from all patients with a LiMON monitor (Pulsion Medical Systems AG, Munich, Germany). DRI was calculated using a previously validated formula. Normally distributed variables were compared using Student's t test. Otherwise, the Mann-Whitney U test or Kruskal-Wallis test was applied, depending on whether there were 2 or more comparable groups. The qualitative variables and risk measurements were analyzed using the chi-square test. P < .05 was considered statistically significant. RESULTS: DRI score (mean ± SD) was 1.58 ± 0.31. The group with DRI >1.7 (poor quality) had an intraoperative arterial flow of 234.2 ± 121.35 mL/min compared with the group having DRI < 1.7 (high quality), with an intraoperative arterial flow of 287.24 ± 156.84 mL/min (P = .02). The group with DRI >1.70 had an ICG-PDR of 14.75 ± 6.52%/min at 60 minutes after reperfusion compared to the group with DRI <1.70, with an ICG-PDR of 16.68 ± 6.47%/min at 60 minutes after reperfusion (P = .09). CONCLUSION: Poor quality grafts have greater susceptibility to ischemia-reperfusion damage. Decreased intraoperative hepatic arterial flow may represent an increase in intrahepatic resistance early in the intraoperative period.

Are high-sensitivity troponins always reliable? Donor-recipient troponin transfusion in liver transplantation.

Blood troponins are used to diagnose perioperative myocardial injury and infarction. During liver transplantation, a passive donor-recipient troponin transfer with the graft may result in an increase of troponins in the transplant recipient questioning the diagnosis of myocardial injury. We present a case of liver transplantation with sudden elevation of recipient's serum troponin levels immediately after graft reperfusion and its subsequent normalization in which myocardial damage and other non-ischaemic potential causes were ruled out. Patient consent for publication was obtained prior to submission of the manuscript.

Cardiac output by femoral arterial thermodilution-calibrated pulse contour analysis during liver transplantation: comparison with pulmonary artery thermodilution.

PURPOSE: The continuous monitoring of the cardiac output during liver transplantation (LT) is an essential part of the intraoperative management of the patient's hemodynamics. To verify the accuracy of a new method based on femoral artery thermodilution-calibrated pulse contour analysis (PCCO) during LT, we compared the technique with the results of an intermittent pulmonary artery thermodilution method (ICO). METHOD: A prospective study included 314 paired cardiac output measurements at 10 sampling times in 35 patients undergoing LT. After initial calibration of the pulse contour analysis, no further recalibrations were performed. Bland and Altman's statistical method, one-way ANOVA, and one sample t tests were used for the analysis of the data. A P<.05 was considered significant. RESULTS: There was a small bias 0.18 L x min(-1) (6.29% from the ICO) for the whole sample of paired measurements, associated with 95% limits of agreement of +/-4.72 (68.89%) L x min(-1). The additional analysis showed comparable biases and limits of agreement for any single time in the study period. The difference PCCO-ICO showed a negative sign for ICO >10 L x min(-1) (P<.001) and a positive sign for ICO <5 L x min(-1) (P<.001). It was greater during infusion of a vasoactive drug (P<.001). CONCLUSION: The pulse contour analysis was found to be an unsatisfactory substitute for intermittent thermodilution measurement of cardiac output during the LT.

Response to clamping of the inferior vena cava as a factor for predicting postreperfusion syndrome during liver transplantation.

Postreperfusion syndrome (PRS) is an important cause of hemodynamic deterioration during orthotopic liver transplantation (OLT). We retrospectively studied 94 patients who had undergone OLT in an effort to establish whether the hemodynamic response to clamping of the inferior vena cava (IVC) could be used to predict hemodynamic behavior on reperfusion of the grafted liver. PRS was defined as a decrease in the mean arterial pressure of more than 30% below the baseline value for more than 1 min during the first 5 min after reperfusion of the graft. The patients were divided into two groups: those who developed PRS (PRS group) and those who did not (non-PRS group). We analyzed hemodynamic response before (dissection stage) and after (anhepatic stage) clamping of the IVC. Based on multivariate analysis methods (logistic regression), the percentage of change in the vascular resistance index from before clamping to after clamping of the IVC was an indicator of the risk of developing PRS, with an adjusted odds ratio of 1.04 for each unit of change (ENTER method, P = 0.01). In the non-PRS group, clamping of the IVC was followed by a 47.1% decrease in the cardiac index, compared with a 27.9% decrease in the PRS group (P < 0.05). The systemic vascular resistance index (SVRI) increased by 49% in the PRS group, as opposed to 85.7% in the non-PRS group (P < 0.05). PRS occurred in only 17.5% of patients in whom the SVRI increased by more than 50%. We conclude that the integrity of the vasoconstrictive response (increase in the peripheral vascular resistance greater than 50%) as measured immediately after clamping of the IVC correlates with occurrence of PRS.

[Internal jugular vein catheterization performed by resident and staff physicians]. / Cateterización de la vena yugular interna realizada por médicos residentes y adjuntos.

OBJECTIVES: To analyse complications in the catheterization of the internal jugular vein using the Boulanger technique and to establish a rating of difficulty and risk when the procedure is carried out by physicians in training. MATERIAL AND METHODS: This was a prospective study of 296 internal jugular vein (IJV) catheterizations by the Boulanger technique carried out by physicians in training (group R2 and group R3-4) or by departmental staff physicians (group staff). Time taken for venous catheterization, rate of success and complications were recorded for each physician performing the procedure. RESULTS: The complication most often observed (11.4%) was puncture of the carotid artery (14.3% group R2, 10% group R3-4 and 8.2% staff), followed by arrhythmia upon insertion of the metal guide (1.6%). There were no instances of pneumothorax or hemothorax, nor any other of the early complications considered infrequent. Success ranged from 68.8% for group R2 to 85.7% for staff. Mean time used in group R2 was 238.7 seconds, while for staff it was 118.3 seconds. CONCLUSION: We suggest that the Boulanger technique for catheterization of the internal jugular vein is a good one and is not particularly hazardous when performed by resident physicians in training.

[Intracavitary electrocardiography. A useful method for checking the correct localization of central venous catheters]. / Electrocardiografía intracavitaria. Un método útil para la comprobación de la correcta colocación de los catéteres venosos centrales.

A placement technique for central venous catheters (CVC) using the intracavitary electrocardiography (ICECG) as well as three different connection systems of the CVC to the electrocardiographic monitor are described. The aim of the present study was to evaluate the correct placement of the CVC by this technique with posterior radiologic confirmation being carried out. The study was undertaken in 30 patients connecting a CVC to a negative electrode of the standard lead II and the positive to the left leg. The CVC was advanced and the changes in the morphology of the "P" wave as it passed along the superior vena cava (SVC) to the right auricle (RA) were observed. In 28 of the 30 patients (93.3%) a biphasic "P" wave (right auricle) was achieved with the CVC being thereafter withdrawn until the SVC (this location was radiologically confirmed posteriorly). In 2 patients (6.6%) a biphasic "P" wave was not obtained and an abnormal position of the CVC was radiologically demonstrated at surgery (one in the ipsilateral subclavian vein and the other had a ring within the right subclavian vein impeding progression). The mean time used in the performance of this technique was 220 +/- 40 s. It is concluded that intracavitary electrocardiography is a simple, easy to learn and perform technique which does not delay surgical procedure and it is a reliable method for placing the end of the CVC.

[Effect of an intravenous nitroglycerin bolus on the hemodynamic impact of laryngoscopy and intubation]. / Efecto de la nitroglicerina intravenosa en bolo sobre la repercusión hemodinámica de la laringoscopia e intubación.

The aim of this study was to evaluate the effectiveness of intravenous administration of a single dose of nitroglycerin in lessening the hemodynamic effects induced during laryngoscopy and tracheal intubation. In an initial subset of 8 patients we verified that the hemodynamic changes after an intravenous dose of 2, 5, or 10 micrograms/kg of nitroglycerin were comparable. The study included 30 patients with a good clinical condition who were anesthetized with fentanyl, thiopental sodium and succinylcholine. They were allocated into two groups of 15 patients according to the intravenous administration or not of 2 micrograms/kg of nitroglycerin after induction of anesthesia. Increase in systolic blood pressure (SBP) and double product (SBP x heart rate) during laryngoscopy and 15, 30, and 45 seconds thereafter was significantly lower in nitroglycerin treated patients than in controls. Increase in diastolic blood pressure was also lower in nitroglycerin treated patients but this difference was only present during laryngoscopy. There were no significant heart rate differences among the two groups of patients. It is concluded that a single intravenous dose of 2 micrograms/kg of nitroglycerin was able to lessen the increase in blood pressure induced by laryngoscopy and tracheal intubation without deleterious effects.

[Massive transfusion: methods and complications]. / Transfusión masiva: métodos y complicaciones.

Massive blood transfusion is a therapeutic procedure increasingly common in anesthesiologic practice. In the present study we reviewed the literature on cellular changes and biochemical abnormalities developing in the blood components during storage. We also reviewed the different methods for infusion and the flow achieved depending on the type of intravenous catheter, infusion system, pressure methods, characteristics of the fluid, and type of filter. We also assessed the pathophysiology of the complications of massive blood transfusion: abnormalities of coagulation, metabolism, oxygen transportation, pulmonary function, hemodynamics and erythrocyte shape, plasma proteins denaturalization, toxicity of plastic products and hypoglycemia.

[Pulmonary atelectasis in bent lateral decubital position (nephrectomy). Apropos of a case]. / Atelectasia pulmonar en la posición de decúbito lateral flexionado (nefrectomía). A propósito de un caso.

Here we have the case of a right nephrectomy ureterectomy for urothelial neoformations in the upper urinary apparatus, in the position of a left flexed lateral decubitus (nephrectomy) practised to a patient, in which a small right pleural aperture was unnoticed until the end of the operation when the closing was being carried out. In the immediate postoperative, the patient developed hypoxia and hypercapnia, as well as an atelectasis of the lower lobus in the left lung, that appeared in the radiological test. We comment now the causes that could have originated this picture, such as overweight, the position of the patient during the operation, its length and the pleural aperture throughout the surgical act, focusing the study in this latter point.