ABSTRACT
Cultivation of the fungus Polyporus squamosus for pectinase production was studied in a polyethylene glycol/crude dextran aqueous two-phase system, with sugar beet extraction waste as pectin source. Fungal growth was restricted to the bottom phase and the amounts of biomass and exo-pectinase activity produced were superior to in homogeneous cultivation. The partition coefficients of endo-pectinase and exo-pectinase were 4.26 and 2.78, respectively. The top phase yields in the single extraction step were about 90% for both pectinases.
Subject(s)
Chenopodiaceae/chemistry , Polygalacturonase/biosynthesis , Polyporaceae/enzymology , Polyporaceae/growth & development , Culture Media/chemistry , Dextrans/metabolism , Fungal Proteins/biosynthesis , Fungal Proteins/isolation & purification , Pectins/metabolism , Plant Extracts/metabolism , Polyethylene Glycols/metabolism , Polygalacturonase/isolation & purificationABSTRACT
The ability of Polyporus squamosus to grow and produce pectinases in an aqueous two-phase medium composed of polyethylene glycol and crude dextran is reported. Fungal growth was restricted to the bottom phase leaving the top phase cell free. Amounts of produced biomass and endo and exo-pectinase activities were superior or equal to those obtained in homogeneous medium. The partition coefficient for the endo-pectinase was 1.52 followed by a top phase yield of 70.86%. Although the phase system composition favours partition of a greater part of exo-pectinase activity to the bottom phase (K(exo) was 0.6 and yield in top phase 48.56%) the partitioned activity in the top phase was equal to that produced in homogeneous cultivation.
