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Aust Endod J ; 30(1): 20-2, 2004 Apr.
Article in English | MEDLINE | ID: mdl-15116905

ABSTRACT

This laboratory study evaluated Er:YAG laser antibacterial action in infected root canals. Forty-eight maxillary central incisors were used. After canal preparation, the teeth were autoclaved and divided into four groups: (1) non-treated teeth (control group); (2) teeth treated with NaOCl; (3) teeth irradiated with Er:YAG laser (7 Hz, 100 mJ, 80 pulses/canal, 11 sec) to the working length; (4) teeth irradiated similarly to, but 3 mm short, of the apex. The root canals from Groups 2, 3 and 4 were inoculated with 4 bacteria: Bacillus subtillus, Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus, together with Candida albicans, and maintained for 24 h at 37 degrees C. All suspensions were adjusted to tube 2 of the MacFarland scale. The intracanal material was then collected with sterile paper points, which were placed in the canals for 5 min and then immersed in 5 ml of BHI medium. This was then seeded onto agar and stained by Gram's method. The NaOCl solutions and the Er:YAG laser irradiation to working length were effective against all five micro-organisms; however, 70% of the specimens irradiated 3 mm short of the apex remained infected.


Subject(s)
Dental Pulp Cavity/microbiology , Disinfection/methods , Laser Therapy , Root Canal Irrigants/therapeutic use , Sodium Hypochlorite/therapeutic use , Aluminum Silicates , Bacillus subtilis/drug effects , Bacillus subtilis/radiation effects , Candida albicans/drug effects , Candida albicans/radiation effects , Colony Count, Microbial , Enterococcus faecalis/drug effects , Enterococcus faecalis/radiation effects , Erbium , Humans , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/radiation effects , Root Canal Preparation , Staphylococcus aureus/drug effects , Staphylococcus aureus/radiation effects , Tooth Apex/microbiology , Yttrium
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