Design, Implementation, and Characterization of a Signal Acquisition Chain for SADino: The Precursor of the Italian Low-Frequency Telescope Named the Sardinia Aperture Array Demonstrator (SAAD).

Low-frequency aperture arrays represent sensitive instruments to detect signals from radio astronomic sources situated in the universe. In Italy, the Sardinia Aperture Array Demonstrator (SAAD) consists of an ongoing project of the Italian National Institute for Astrophysics (INAF) aimed to install an aperture array constituted of 128 dual-polarized Vivaldi antennas at the Sardinia Radio Telescope (SRT) site. The originally envisaged 128 elements of SAAD were re-scoped to the 16 elements of its precursor named SADino, with the aim to quickly test the system with a digital beam-former based on the Italian Tile Processing Module (iTPM) digital back-end. A preliminary measurements campaign of radio frequency interference (RFI) was performed to survey the less contaminated spectral region. The results of these measurements permitted the establishment of the technical requirements for receiving a chain for the SADino telescope. In this paper, the design, implementation, and characterization of this signal acquisition chain are proposed. The operative frequency window of SAAD and its precursor, SADino, sweeps from 260 MHz to 420 MHz, which appears very attractive for radio astronomy applications and radar observation in space and surveillance awareness (SSA) activities.

Upgrading of the L-P Band Cryogenic Receiver of the Sardinia Radio Telescope: A Feasibility Study.

The Sardinia Radio Telescope is a quasi-Gregorian system with a shaped 64 m diameter primary reflector and a 7.9 m diameter secondary reflector. It was designed to operate with high efficiency across the 0.3-116 GHz frequency range. The telescope is equipped with a cryogenic coaxial dual-frequency L-P band receiver, which covers a portion of the P-band (305-410 MHz) and the L-band (1300-1800 MHz). Although this receiver has been used for years in its original design, with satisfactory results, it presents some parts that could be upgraded in order to improve the performances of the system. With the passing of time and with technology advances, the presence of unwanted human-made signals in the area around the telescope, known as radio frequency interferences, has grown exponentially. In addition, the technology of the receiver electronic control system became obsolete and it could be replaced with next-generation electronic boards, which offer better performances both service reliability and low generation of unwanted radio frequency signals. In this paper, a feasibility study for improving the L-P band receiver is discussed, taking into account the mitigation of the main radio frequency interferences. With this study, it is possible to have a sensitive instrument that can be used for scientific research at low frequencies (P- and L-bands), which are usually populated by signals from civil and military mobile communications, TV broadcasting and remote sensing applications.

A high resolution melting method for the molecular identification of the potentially toxic diatom Pseudo-nitzschia spp. in the Mediterranean Sea.

The aim of this study was to develop and validate a high resolution melting (HRM) method for the rapid, accurate identification of the various harmful diatom Pseudo-nitzschia species in marine environments. Pseudo-nitzschia has a worldwide distribution and some species are toxic, producing the potent domoic acid toxin, which poses a threat to both human and animal health. Hence, it is important to identify toxic Pseudo-nitzschia species. A pair of primers targeting the LSU rDNA of the genus Pseudo-nitzschia was designed for the development of the assay and its specificity was validated using 22 control DNAs of the P. calliantha, P. delicatissima/P. arenysensis complex and P. pungens. The post-PCR HRM assay was applied to numerous unidentified Pseudo-nitzschia strains isolated from the northwestern Adriatic Sea (Mediterranean Sea), and it was able to detect and discriminate three distinct Pseudo-nitzschia taxa from unidentified samples. Moreover, the species-specific identification of Pseudo-nitzschia isolates by the HRM assay was consistent with phylogenetic analyses. The HRM assay was specific, robust and rapid when applied to high numbers of cultured samples in order to taxonomically identify Pseudo-nitzschia isolates recovered from environmental samples.

A VLBI experiment using a remote atomic clock via a coherent fibre link.

We describe a VLBI experiment in which, for the first time, the clock reference is delivered from a National Metrology Institute to a radio telescope using a coherent fibre link 550 km long. The experiment consisted of a 24-hours long geodetic campaign, performed by a network of European telescopes; in one of those (Medicina, Italy) the local clock was alternated with a signal generated from an optical comb slaved to a fibre-disseminated optical signal. The quality of the results obtained with this facility and with the local clock is similar: interferometric fringes were detected throughout the whole 24-hours period and it was possible to obtain a solution whose residuals are comparable to those obtained with the local clock. These results encourage further investigation of the ultimate VLBI performances achievable using fibre dissemination at the highest precision of state-of-the-art atomic clocks.

A coherent fiber link for very long baseline interferometry.

We realize a coherent fiber link for application in very long baseline interferometry (VLBI) for radio astronomy and geodesy. A 550-km optical fiber connects the Italian National Metrological Institute (INRIM) to a radio telescope in Italy and is used for the primary Cs fountain clock stability and accuracy dissemination. We use an ultrastable laser frequency- referenced to the primary standard as a transfer oscillator; at the radio telescope, an RF signal is generated from the laser by using an optical frequency comb. This scheme now provides the traceability of the local maser to the SI second, realized by the Cs fountain at the 1.7 × 10(-16) accuracy. The fiber link never limits the experiment and is robust enough to sustain radio astronomical campaigns. This experiment opens the possibility of replacing the local hydrogen masers at the VLBI sites with optically-synthesized RF signals. This could improve VLBI resolution by providing more accurate and stable frequency references and, in perspective, by enabling common- clock VLBI based on a network of telescopes connected by fiber links.

The sxt Gene and Paralytic Shellfish Poisoning Toxins as Markers for the Monitoring of Toxic Alexandrium Species Blooms.

Paralytic shellfish poisoning (PSP) is a serious human illness caused by the ingestion of seafood contaminated with saxitoxin and its derivatives (STXs). These toxins are produced by some species of marine dinoflagellates within the genus Alexandrium. In the Mediterranean Sea, toxic Alexandrium spp. blooms, especially of A. minutum, are frequent and intense with negative impact to coastal ecosystem, aquaculture practices and other economic activities. We conducted a large scale study on the sxt gene and toxin distribution and content in toxic dinoflagellate A. minutum of the Mediterranean Sea using both quantitative PCR (qPCR) and HILIC-HRMS techniques. We developed a new qPCR assay for the estimation of the sxtA1 gene copy number in seawater samples during a bloom event in Syracuse Bay (Mediterranean Sea) with an analytical sensitivity of 2.0 × 10° sxtA1 gene copy number per reaction. The linear correlation between sxtA1 gene copy number and microalgal abundance and between the sxtA1 gene and STX content allowed us to rapidly determine the STX-producing cell concentrations of two Alexandrium species in environmental samples. In these samples, the amount of sxtA1 gene was in the range of 1.38 × 10(5) - 2.55 × 10(8) copies/L and the STX concentrations ranged from 41-201 nmol/L. This study described a potential PSP scenario in the Mediterranean Sea.

SxtA and sxtG gene expression and toxin production in the Mediterranean Alexandrium minutum (Dinophyceae).

The dinoflagellate Alexandrium minutum is known for the production of potent neurotoxins affecting the health of human seafood consumers via paralytic shellfish poisoning (PSP). The aim of this study was to investigate the relationship between the toxin content and the expression level of the genes involved in paralytic shellfish toxin (PST) production. The algal cultures were grown both in standard f/2 medium and in phosphorus/nitrogen limitation. In our study, LC-HRMS analyses of PST profile and content in different Mediterranean A. minutum strains confirmed that this species was able to synthesize mainly the saxitoxin analogues Gonyautoxin-1 (GTX1) and Gonyautoxin-4 (GTX4). The average cellular toxin content varied among different strains, and between growth phases, highlighting a decreasing trend from exponential to stationary phase in all culture conditions tested. The absolute quantities of intracellular sxtA1 and sxtG mRNA were not correlated with the amount of intracellular toxins in the analysed A. minutum suggesting that the production of toxins may be regulated by post-transcriptional mechanisms and/or by the concerted actions of alternative genes belonging to the PST biosynthesis gene cluster. Therefore, it is likely that the sxtA1 and sxtG gene expression could not reflect the PST accumulation in the Mediterranean A. minutum populations under the examined standard and nutrient limiting conditions.

Monitoring toxic Ostreopsis cf. ovata in recreational waters using a qPCR based assay.

Ostreopsis sp. is a toxic marine benthic dinoflagellate that causes high biomass blooms, posing a threat to human health, marine biota and aquaculture activities, and negatively impacting coastal seawater quality. Species-specific identification and enumeration is fundamental because it can allow the implementation of all the necessary preventive measures to properly manage Ostreopsis spp. bloom events in recreational waters and aquaculture farms. The aim of this study was to apply a rapid and sensitive qPCR method to quantify Ostreopsis cf. ovata abundance in environmental samples collected from Mediterranean coastal sites and to develop site-specific environmental standard curves. Similar PCR efficiencies of plasmid and environmental standard curves allowed us to estimate the LSU rDNA copy number per cell. Moreover, we assessed the effectiveness of mitochondrial COI and cob genes as alternative molecular markers to ribosomal genes in qPCR assays for Ostreopsis spp. quantification.

New approach using the real-time PCR method for estimation of the toxic marine dinoflagellate Ostreopsis cf. ovata in marine environment.

BACKGROUND: We describe the development and validation of a new quantitative real time PCR (qrt-PCR) method for the enumeration of the toxic benthic dinoflagellate Ostreopsis cf. ovata in marine environment. The benthic Ostreopsis sp. has a world-wide distribution and is associated during high biomass proliferation with the production of potent palytoxin-like compounds affecting human health and environment. Species-specific identification, which is relevant for the complex of different toxins production, by traditional methods of microscopy is difficult due to the high morphological variability, and thus different morphotypes can be easily misinterpreted. METHODOLOGY/FINDINGS: The method is based on the SYBR I Green real-time PCR technology and combines the use of a plasmid standard curve with a "gold standard" created with pooled crude extracts from environmental samples collected during a bloom event of Ostreopsis cf. ovata in the Mediterranean Sea. Based on their similar PCR efficiencies (95% and 98%, respectively), the exact rDNA copy number per cell was obtained in cultured and environmental samples. Cell lysates were used as the templates to obtain total recovery of DNA. The analytical sensitivity of the PCR was set at two rDNA copy number and 8.0×10(-4) cell per reaction for plasmid and gold standards, respectively; the sensitivity of the assay was of cells g(-1) fw or 1(-1) in macrophyte and seawater samples, respectively. The reproducibility was determined on the total linear quantification range of both curves confirming the accuracy of the technical set-up in the complete ranges of quantification over time. CONCLUSIONS/SIGNIFICANCE: We developed a qrt-PCR assay specific, robust and high sample throughput for the absolute quantification of the toxic dinoflagellate Ostreopsis cf. ovata in the environmental samples. This molecular approach may be considered alternative to traditional microscopy and applied for the monitoring of benthic toxic microalgal species in the marine ecosystems.