1.
Chem Commun (Camb)
; 51(63): 12548-51, 2015 Aug 14.
Article
in English
| MEDLINE
| ID: mdl-26152789
ABSTRACT
A modified sortase A recognition motif containing a masked Ni(2+)-binding peptide was employed to boost the efficiency of sortase-catalyzed ligation reactions. Deactivation of the Ni(2+)-binding peptide using a Ni(2+) additive improved reaction performance at low to equimolar ratios of the glycine amine nucleophile and sortase substrate. The success of this approach was demonstrated with both peptide and protein substrates.