ABSTRACT
The review presents experimental data on the integration in the plant nuclear genome of vector sequences during Agrobacterium-mediated transformation. Main attention is given to the frequency and mechanisms of this event among transgenic plants. The factors influencing the frequency of this event, as well as the ways of developing special constructs for elimination of vector backbone sequences as undesirable from the point of view of commercial use of genetically modified plants, are considered.
Subject(s)
Gene Transfer Techniques , Genetic Vectors/genetics , Genome, Plant/physiology , Plants/genetics , Rhizobium/genetics , Transformation, Genetic , Plants, Genetically Modified/geneticsABSTRACT
Upon processing of T-DNA, both borders may be recognized incorrectly. A number of transgenic plants containing, apart from a fragment of vector DNA adjacent to the left border of T-DNA, a vector fragment inserted irrespective of it were identified. Sequence analysis confirmed that genes located beyond the boundaries of T-DNA are transferred and inserted in the genome of transgenic plants. Recombination of vector DNA was discovered among plants of the first generation after self-pollination of the original transformants.
Subject(s)
Genetic Vectors/genetics , Genome, Plant/genetics , Plants, Genetically Modified/genetics , Recombination, Genetic , Transgenes , Base Sequence , Molecular Sequence Data , Plasmids/genetics , Sequence Analysis, DNA , Nicotiana/geneticsABSTRACT
The authors evaluated influence of Alfetin new Russian biologic preparation on pulmonary surfactant system and phospholipid metabolism in workers at risk of occupational pneumoconiosis, who suffer from infiltrative pulmonary tuberculosis. Considering positive changes in serum phospholipids subunits under alfetin treatment, the authors proved membrane protective effect of the drug. Alfetin was proved to weaken toxic effects of tuberculostatic drugs on lung surfactant.
