ABSTRACT
Ndel1 and Nde1 are homologous and evolutionarily conserved proteins, with critical roles in cell division, neuronal migration, and other physiological phenomena. These functions are dependent on their interactions with the retrograde microtubule motor dynein and with its regulator Lis1--a product of the causal gene for isolated lissencephaly sequence (ILS) and Miller-Dieker lissencephaly. The molecular basis of the interactions of Ndel1 and Nde1 with Lis1 is not known. Here, we present a crystallographic study of two fragments of the coiled-coil domain of Ndel1, one of which reveals contiguous high-quality electron density for residues 10-166, the longest such structure reported by X-ray diffraction at high resolution. Together with complementary solution studies, our structures reveal how the Ndel1 coiled coil forms a stable parallel homodimer and suggest mechanisms by which the Lis1-interacting domain can be regulated to maintain a conformation in which two supercoiled alpha helices cooperatively bind to a Lis1 homodimer.
Subject(s)
1-Alkyl-2-acetylglycerophosphocholine Esterase/chemistry , Carrier Proteins/chemistry , Microtubule-Associated Proteins/chemistry , 1-Alkyl-2-acetylglycerophosphocholine Esterase/metabolism , Amino Acid Sequence , Carrier Proteins/metabolism , Circular Dichroism , Classical Lissencephalies and Subcortical Band Heterotopias/metabolism , Crystallography, X-Ray , Dimerization , Humans , Microtubule-Associated Proteins/metabolism , Models, Biological , Models, Molecular , Molecular Sequence Data , Protein Structure, Tertiary , Sequence AlignmentABSTRACT
The helicase-associated endonuclease for fork-structured DNA (Hef) is an archaeabacterial protein that processes blocked replication forks. Here we have isolated the vertebrate Hef ortholog and investigated its molecular function. Disruption of this gene in chicken DT40 cells results in genomic instability and sensitivity to DNA cross-links. The similarity of this phenotype to that of cells lacking the Fanconi anemia-related (FA) tumor-suppressor genes led us to investigate whether Hef functions in this pathway. Indeed, we found a genetic interaction between the FANCC and Hef genes. In addition, Hef is a component of the FA nuclear protein complex that facilitates its DNA damage-inducible chromatin localization and the monoubiquitination of the FA protein FANCD2. Notably, Hef interacts directly with DNA structures that are intermediates in DNA replication. This discovery sheds light on the origins, regulation and molecular function of the FA tumor-suppressor pathway in the maintenance of genome stability.
Subject(s)
Avian Proteins/metabolism , Cell Cycle Proteins/metabolism , Chickens , Conserved Sequence , DNA-Binding Proteins/metabolism , Fanconi Anemia/metabolism , Nuclear Proteins/metabolism , Tumor Suppressor Proteins/metabolism , Adenosine Triphosphatases/metabolism , Animals , Avian Proteins/chemistry , Avian Proteins/deficiency , Avian Proteins/genetics , Cell Cycle Proteins/genetics , Cell Line , Chickens/genetics , Chickens/metabolism , DNA/metabolism , DNA Damage , DNA Helicases/metabolism , DNA Repair , DNA Replication , DNA-Binding Proteins/deficiency , DNA-Binding Proteins/genetics , Endonucleases/genetics , Endonucleases/metabolism , Evolution, Molecular , Fanconi Anemia/genetics , Fanconi Anemia Complementation Group C Protein , Fanconi Anemia Complementation Group Proteins , Genomic Instability , Humans , Nuclear Proteins/chemistry , Nuclear Proteins/deficiency , Nuclear Proteins/genetics , Tumor Suppressor Proteins/geneticsABSTRACT
Mutations in the LIS1 gene cause lissencephaly, a human neuronal migration disorder. LIS1 binds dynein and the dynein-associated proteins Nde1 (formerly known as NudE), Ndel1 (formerly known as NUDEL), and CLIP-170, as well as the catalytic alpha dimers of brain cytosolic platelet activating factor acetylhydrolase (PAF-AH). The mechanism coupling the two diverse regulatory pathways remains unknown. We report the structure of LIS1 in complex with the alpha2/alpha2 PAF-AH homodimer. One LIS1 homodimer binds symmetrically to one alpha2/alpha2 homodimer via the highly conserved top faces of the LIS1 beta propellers. The same surface of LIS1 contains sites of mutations causing lissencephaly and overlaps with a putative dynein binding surface. Ndel1 competes with the alpha2/alpha2 homodimer for LIS1, but the interaction is complex and requires both the N- and C-terminal domains of LIS1. Our data suggest that the LIS1 molecule undergoes major conformational rearrangement when switching from a complex with the acetylhydrolase to the one with Ndel1.
