Subject(s)
Hyperbaric Oxygenation , Multiple Sclerosis/therapy , Animals , Blood-Brain Barrier/physiology , Embolism, Fat/complications , Encephalomyelitis, Autoimmune, Experimental , Guinea Pigs , Humans , Models, Animal , Multiple Sclerosis/etiology , Multiple Sclerosis/physiopathology , Randomized Controlled Trials as TopicABSTRACT
The effects of hyperbaric oxygen at a pressure of two atmospheres absolute were studied in a group of patients with chronic progressive multiple sclerosis. A slight but statistically insignificant shortening of the visual evoked potential latencies was seen after treatment with hyperbaric oxygen as compared with placebo treatment. The treatment did not appreciably halt the progression of the disease and deferioration occurred more often among the patients in the treatment group than in the control group.
Subject(s)
Hyperbaric Oxygenation , Multiple Sclerosis/therapy , Adult , Clinical Trials as Topic , Double-Blind Method , Evoked Potentials, Visual , Female , Humans , Male , Multiple Sclerosis/physiopathology , Reaction Time , Visual Pathways/physiopathologyABSTRACT
As mature mammalian erythrocytes do not contain nuclei, erythrocyte chalone has previously been detected by its inhibitory effect on normoblasts, often obtained from a mixed cell source. Chick embryo blood consists entirely of mature nucleated red cells and was used to test the effect of heterologous erythrocytic extracts on haemopoiesis. The model was adapted to detect the presence of inhibitors of erythropoiesis as contaminants of partially purified leucocyte extracts.
Subject(s)
Erythrocytes/metabolism , Growth Inhibitors , Animals , Cattle , Chick Embryo , DNA/biosynthesis , Epithelium/metabolism , Erythropoiesis , Intestinal Mucosa/metabolism , Leukocytes/metabolism , SheepABSTRACT
An in vivo system has been developed for estimating the inhibitory activities of granulocyte chalone which overcomes the objection to the use of mixed proliferating target cells. It depends on the identification of labelled mature granulocytes in the peripheral blood of mice that received a radioactive precursor of DNA synthesis, with and without granulocyte extracts, several days previously. Two extracts of bovine granulocytes were tested. The first demonstrated that maximum inhibition was achived when it was given 2 h before the isotope, suggesting that its activity was short lived. It also failed to depress the labelling index of peripheral lymphocytes, thereby demonstrating tissue specificity. The second was used to show that the dose-response relationship was exponential.
Subject(s)
Granulocytes/analysis , Growth Inhibitors , Animals , Dose-Response Relationship, Drug , Female , Growth Inhibitors/pharmacology , Leukocyte Count , Lymphocytes/metabolism , Mice , Mice, Inbred DBA , Thymidine/metabolismABSTRACT
In a randomized controlled clinical trial of hyperbaric oxygen in the radiotherapy of carcinoma of the bladder a total of 241 cases were contributed by four radiotherapy centres in the United Kingdom. In this trial where in each centre identical radiotherapy was employed for both oxygen and air cases, no benefit was shown with the use of hyperbaric oxygen.
Subject(s)
Hyperbaric Oxygenation , Urinary Bladder Neoplasms/radiotherapy , Air , Clinical Trials as Topic , Humans , Radiotherapy Dosage , Random Allocation , Urinary Bladder Neoplasms/mortalityABSTRACT
In a randomized controlled clinical trial of hyperbaric oxygen in the radiotherapy of advanced carcinoma of the uterine cervix a total of 320 cases were contributed by four radiotherapy centres in the United Kingdom. The use of hyperbaric oxygen resulted in improved local control and survival. The benefit was greatest in patients under the age of 55 who presented with stage III disease. There was a slight increase in radiation morbidity but it seemed that the benefit of hyperbaric oxygen outweighed this increase in morbidity and that there was a true improvement in the therapeutic ratio.
