ABSTRACT
Nine Trypanosoma sp. Venezuelan isolates, initially presumed to be T. evansi, were collected from three different hosts, capybara (Apure state), horse (Apure state) and donkey (Guarico state) and compared by the random amplification polymorphic DNA technique (RAPD). Thirty-one to 46 reproducible fragments were obtained with 12 of the 40 primers that were used. Most of the primers detected molecular profiles with few polymorphisms between the seven horse, capybara and donkey isolates. Quantitative analyses of the RAPD profiles of these isolates revealed a high degree of genetic conservation with similarity coefficients between 85.7% and 98.5%. Ten of the primers generated polymorphic RAPD profiles with two of the three Trypanosoma sp. horse isolates, namely TeAp-N/D1 and TeGu-N/D1. The similarity coefficient between these two isolates and the rest, ranged from 57.9% to 68.4% and the corresponding dendrogram clustered TeAp-N/D1 and Te Gu-N/D1 in a genetically distinct group.
Subject(s)
DNA, Protozoan/genetics , Random Amplified Polymorphic DNA Technique , Trypanosoma/genetics , Trypanosoma/isolation & purification , Animals , Base Sequence , DNA, Protozoan/classification , Genetic Variation , Phylogeny , Trypanosoma/classification , VenezuelaABSTRACT
Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts.
Subject(s)
Parasitemia/veterinary , Sheep Diseases/parasitology , Trypanosoma vivax/isolation & purification , Trypanosomiasis, African/veterinary , Animals , Centrifugation, Isopycnic/veterinary , Chromatography, DEAE-Cellulose/veterinary , Immunosuppression Therapy , Parasitemia/immunology , Parasitemia/parasitology , Protozoan Proteins/analysis , Sheep , Sheep Diseases/immunology , Trypanosoma vivax/chemistry , Trypanosomiasis, African/immunology , Trypanosomiasis, African/parasitologyABSTRACT
The present work was undertaken to evaluate whether changes in cell water content of rat kidney cortex cells can modulate the transport activity of the ouabain-insensitive Na pump as they modulate the ouabain-insensitive Na+-ATPase. It was found that there is a close relationship between the cell volume and activity of the Na pump, whereas Na,K-pump activity is not affected by variations in cell volume. When the cell water content is low, Na-pump activity (Na+ transport and Na+-ATPase activity) is minimal. Increases in cell water content produce a concomitant increase in Na-pump activity.
