ABSTRACT
Background and objective Healthcare-associated infections (HAIs), especially central line-associated bloodstream infections (CLABSI), are among the most critical public health problems worldwide. Knowledge, attitude, and skills of nurses are vital in HAI prevention. In this study, we aimed to assess nurses' knowledge, skills, and compliance related to CLABSI. Method This study was conducted in a heart center as a prospective interventional study. Eighty nurses were selected after obtaining their consent to participate in the pretest, posttest, and skills review. Qualified nurses registered with the Saudi Council and working for at least one month in the relevant unit at the time of the study were included. Nurse managers, interns, and student nurses were excluded. Nurses' skills were analyzed using a competency-based checklist approved by the hospital. Results We enrolled 80 participants in our study. The majority of the participants (51.25%) fell under the age group of 25-34 years. There were 68 females (85%). Participants with an experience of 6-10 years constituted the biggest proportion (37.5%) in the cohort in terms of work experience. The mean CLABSI knowledge-related pretest and posttest scores were 6.7 ±1.09 and 6.8 ±1.11, respectively, while the CLABSI compliance scores were 8.1 ±0.99 and 8.3 ±0.97, respectively. Conclusion Based on our findings, clinical experience of more than five years is associated with good CLABSI knowledge and compliance among nurses. Nurses' level of education also had a significant relationship with CLABSI pretest and posttest scores.
ABSTRACT
Doxorubicin (DOXO) remains amongst the most commonly used anti-cancer agents for the treatment of solid tumors, lymphomas, and leukemias. However, its clinical use is hampered by cardiotoxicity, characterized by heart failure and arrhythmias, which may require chemotherapy interruption, with devastating consequences on patient survival and quality of life. Although the adverse cardiac effects of DOXO are consolidated, the underlying mechanisms are still incompletely understood. It was previously shown that DOXO leads to proteotoxic cardiomyocyte (CM) death and myocardial fibrosis, both mechanisms leading to mechanical and electrical dysfunction. While several works focused on CMs as the culprits of DOXO-induced arrhythmias and heart failure, recent studies suggest that DOXO may also affect cardiac sympathetic neurons (cSNs), which would thus represent additional cells targeted in DOXO-cardiotoxicity. Confocal immunofluorescence and morphometric analyses revealed alterations in SN innervation density and topology in hearts from DOXO-treated mice, which was consistent with the reduced cardiotropic effect of adrenergic neurons in vivo. Ex vivo analyses suggested that DOXO-induced denervation may be linked to reduced neurotrophic input, which we have shown to rely on nerve growth factor, released from innervated CMs. Notably, similar alterations were observed in explanted hearts from DOXO-treated patients. Our data demonstrate that chemotherapy cardiotoxicity includes alterations in cardiac innervation, unveiling a previously unrecognized effect of DOXO on cardiac autonomic regulation, which is involved in both cardiac physiology and pathology, including heart failure and arrhythmias.
