ABSTRACT
Influence of 4-E-BPE on the amplitude of population spices (PS) evoked in CA1 area by Shaffer collateral stimulation in hippocampal slices were analysed. Bath application of 4-E-BPE (10(-6)-10(-5) M) led to a pronounced increase in the amplitude of the PS, the appearance of secondary PS and then introduction of GABA led to restoring original state. The 4-E-BPE was more potent than picrotoxin. These findings suggest that 4-E-BPE suppress inhibitory synaptic transmission in the CA1 region of hippocampus.
Subject(s)
Bridged Bicyclo Compounds, Heterocyclic , Bridged Bicyclo Compounds/pharmacology , GABA Antagonists , Hippocampus/physiology , Neurons/physiology , Organophosphorus Compounds/pharmacology , Synapses/physiology , Animals , Evoked Potentials , Hippocampus/drug effects , In Vitro Techniques , Mice , Mice, Inbred BALB C , Neurons/drug effects , Picrotoxin/pharmacology , Synapses/drug effectsABSTRACT
DSTA was analyzed for its effect, on the amplitude of population spikes (PS) evoked in CA1 area by the Shaffer collateral stimulation in hippocampal slices. Bath application of DSTA (10(-6)-10(-5) mol/l) caused a pronounced increase in the amplitude of PS and appearance of secondary PS. Introduction of GABA restored it to the initial state. These results demonstrate that DSTA inhibits the synaptic transmission in the CA1 region of the hippocampus.
Subject(s)
Bridged-Ring Compounds/pharmacology , GABA Antagonists , Hippocampus/drug effects , Neurons/drug effects , Animals , Evoked Potentials/drug effects , Hippocampus/physiology , In Vitro Techniques , Mice , Mice, Inbred BALB C , Neurons/physiologySubject(s)
Brain/metabolism , Bridged Bicyclo Compounds/metabolism , Bridged-Ring Compounds/metabolism , Organophosphates/metabolism , Organophosphorus Compounds/metabolism , Picrotoxin/metabolism , gamma-Aminobutyric Acid/metabolism , Allosteric Regulation , Animals , Binding Sites , Cell Membrane/metabolism , Rats , Rats, Inbred StrainsABSTRACT
The properties of artificial lipid membranes modified by frog offactory preparation obtained by ultrasonic treatment of frog olfactory tissues were investigated. Out of the 24 odorous substances which were tested five active stimulants were identified each inducing a resistance drop of the modified membrane when added to the cell. The studies of this effect in solutions with different salt content demonstrated that the decrease in resistance resulted most probably from an increased membrane permeability to Na+ ions. The dyes did not affect the resistance of modified membranes. Mercury bichloride at the concentration of 5 . 10(-4) M was shown to block the responce of the membrane when added to the cell prior to stimulants. At the same time mercury biochloride did not practically affect the membrane resistance after its response to the odorants. The possible ways of increasing the sensitivity of modified membranes to odorants are discussed.
