ABSTRACT
This study was designed to detect HBsAg in bloodstains on evidential objects by solid-phase immunoenzyme assay and to characterize its sensitivity, specificity, and reproducibility as well as effect of various external factors on the results of HBsAg measurements. Both whole blood from 25 HBsAg carriers and its stains were available for analysis. HBsAg was detected in all blood stains regardless of the time of their formation. However, HBsAg was absent in 25 stains of blood from control subjects and in 27 extracts from carrier objects. A highly specific, sensitive, and well-reproducible method was developed for determining HBsAg in traces of blood after its preliminary extraction from the stain. The method allows HBsAg to be detected in a stain containing 4 x 10(-6) ml of blood.
Subject(s)
Blood Stains , Hepatitis B Antigens/analysis , Enzyme-Linked Immunosorbent Assay/methods , Humans , Sensitivity and SpecificitySubject(s)
Clinical Laboratory Techniques , Diagnostic Equipment , Laboratories, Hospital , National Health Programs , Primary Health Care , Clinical Laboratory Techniques/instrumentation , Clinical Laboratory Techniques/standards , Diagnostic Equipment/standards , Laboratories, Hospital/organization & administration , Laboratories, Hospital/standards , National Health Programs/organization & administration , National Health Programs/standards , Primary Health Care/organization & administration , Primary Health Care/standards , RussiaABSTRACT
Anemic syndrome rate in diabetic angiopathy reaching 1/3 in elderly patients, frequency increases after 70 years. The anemic syndrome rate in patients older 60 years suffering from the diabetes mellitus of the 1 type, complicated by "diabetic foot" syndrome and in cases with duration of the disease more than 10 years is elevated. In diabetic angiopathy anemia can be normo- or hypochromic or followed by aniso- or poykilocytosis. Among the causes of anemia in patients with diabetic angiopathies the important place is occupied by mediated by oxidative stress, endotoxicosis and protein glycation prolong hyperglycemia effect on the morphofunctional properties of the red blood cells.