ABSTRACT
Left ventricular hypertrophy is considered a risk for cardiac failure. In an attempt to understand the molecular basis of antioxidant changes during ventricular hypertrophy, we have used isoproterenol treated rats. Our studies showed that administration of high doses of isoproterenol induced oxidative and nitrosative stress, expressed as decreased level of thiols (SH), decreased superoxide dismutase (SOD) activity and increased nitrates level in the rat heart. The results obtained are considered an outcome of oxidative stress conditions imposed by isoproterenol autooxidation.
Subject(s)
Hypertrophy, Left Ventricular/metabolism , Myocardium/metabolism , Nitrates/metabolism , Superoxide Dismutase/metabolism , Adrenergic beta-Agonists , Animals , Hypertrophy, Left Ventricular/chemically induced , Isoproterenol , Male , Oxidative Stress , Rats , Sulfhydryl Compounds/metabolismABSTRACT
Serum albumine from rats at different ages (1, 3, 9, 18 months) undergoes a thermal reversible denaturation in the range of 40-53 degrees C. Thermal transitions of unbuffered solution of native protein show a significant change of pH as they became heat denaturated. The protein molecule has been analysed in melting studies, manifested by cooperative dissociation of protons and it experiences a decrease in temperature transition with the younger the rats.
