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1.
Oncology ; 64(4): 450-8, 2003.
Article in English | MEDLINE | ID: mdl-12759545

ABSTRACT

We have isolated a novel gene, lung cancer-associated gene Y (LAGY), by suppression subtractive hybridization. The nucleotide sequence of LAGY predicts a small protein of 73 amino acids containing a putative homeobox domain with a molecular mass of 8.1 kD. Multiple-tissue Northern blot analysis revealed that LAGY is present in human placenta, lung, brain, heart and skeletal muscle. Gene mapping locates LAGY on chromosome 4q11-13.1. The expression of LAGY mRNA was widely lost in 18 lung tumor cell lines comprising all major histological types, as shown by Northern blot analysis and semiquantitative reverse transcription-polymerase chain reaction. In an investigation of 72 primary lung tumors, this gene was significantly downregulated in tumors compared to 9 normal lung tissue samples. There was a significant reduction of LAGY expression in squamous cell carcinoma (SCC; n = 27) with increasing grade and stage. No expression was detectable in two high-grade SCCs or two small cell and large cell lung carcinomas (n = 4 for each). In adenocarcinoma (n = 37), expression was reduced; however, this did not reach statistical significance. Since homeodomain-containing genes are known to transcriptionally regulate key cellular processes and are associated with carcinogenesis, we suggest that LAGY might be linked to lung cancer development and progression.


Subject(s)
Genes, Homeobox/genetics , Lung Neoplasms/genetics , Lung Neoplasms/pathology , Amino Acid Sequence , Base Sequence , Blotting, Northern , Blotting, Southern , Chromosome Mapping , Chromosomes, Human, Pair 4/genetics , DNA, Complementary/analysis , DNA, Neoplasm/analysis , Down-Regulation , Gene Expression Regulation, Neoplastic , Humans , Molecular Sequence Data , Neoplasm Staging , RNA, Messenger/analysis , RNA, Neoplasm/analysis , Reverse Transcriptase Polymerase Chain Reaction , Tumor Cells, Cultured
3.
J Exp Med ; 196(4): 469-80, 2002 Aug 19.
Article in English | MEDLINE | ID: mdl-12186839

ABSTRACT

Nonreciprocal translocations and gene amplifications are commonly found in human tumors. Although little is known about the mechanisms leading to such aberrations, tissue culture models predict that they can arise from DNA breakage, followed by cycles of chromatid fusion, asymmetric mitotic breakage, and replication. Mice deficient in both a nonhomologous end joining (NHEJ) DNA repair protein and the p53 tumor suppressor develop lymphomas at an early age harboring amplification of an IgH/c-myc fusion. Here we report that these chromosomal rearrangements are initiated by a recombination activating gene (RAG)-induced DNA cleavage. Subsequent DNA repair events juxtaposing IgH and c-myc are mediated by a break-induced replication pathway. Cycles of breakage-fusion-bridge result in amplification of IgH/c-myc while chromosome stabilization occurs through telomere capture. Thus, mice deficient in NHEJ provide excellent models to study the etiology of unbalanced translocations and amplification events during tumorigenesis.


Subject(s)
Antigens, Nuclear , DNA Damage , DNA Helicases , DNA Repair , DNA Replication , DNA-Binding Proteins/physiology , Immunoglobulin Heavy Chains/genetics , Lymphoma, B-Cell/genetics , Proto-Oncogene Proteins c-myc/genetics , Thymus Neoplasms/genetics , Translocation, Genetic , Animals , Base Sequence , DNA-Binding Proteins/genetics , Gene Amplification , Gene Rearrangement , Ku Autoantigen , Mice , Mice, Knockout , Mice, SCID , Molecular Sequence Data , Nuclear Proteins/genetics , Nuclear Proteins/physiology , Oncogenes , Telomere , Tumor Suppressor Protein p53/genetics , Tumor Suppressor Protein p53/physiology
4.
Hum Pathol ; 33(6): 632-41, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12152163

ABSTRACT

The genetic changes involved in the pathogenesis of ovarian carcinoma are not completely understood. To investigate this matter, we studied paraffin-embedded, microdissected tissue of 47 ovarian epithelial tumors (9 adenomas, 11 tumors of low malignant potential [LMP], 14 serous carcinomas, and 13 nonserous carcinomas) using comparative genomic hybridization (CGH). (The primary data used in this study are available at our CGH online tumor database at http://amba.charite.de/cgh.) Chromosomal imbalances were found in 1 serous adenoma and in 7 LMP tumors. In the latter the alterations appeared randomly and showed no overlap with alterations found in invasive carcinomas. Although the mean aberration number of low-grade serous carcinomas was comparable to LMP tumors, the imbalances of the former occurred with high incidence (>50%) and were found at different localizations. High-grade serous carcinomas had more than twice as much chromosomal imbalances as low-grade serous carcinomas and also had pronounced alterations. In serous carcinomas, gains were found on 3q, 6p, 7, 8q, and 20, and losses were found on 4q, 6q, 12q, 13q, and 16q. Comparing serous and nonserous carcinomas, the mean aberration number was comparable, but the number of high incidence changes was lower, and the most frequent imbalances were losses on 13q and gains on 20p. Overlapping alterations occurring in serous and nonserous carcinomas were gains on 3q and 6p, as well as losses on 4q. Chromosomal imbalances associated with poor prognosis of ovarian carcinomas were gains on 6p, 7q, and 13q and losses on 15q, 17p, 18q, and 21q. Our data indicate that serous LMP tumors and invasive carcinomas have different genetic aberrations, indicating that invasive carcinomas do not arise from preexisting serous LMP tumors. On the other hand, there are common genetic abnormalities in serous and nonserous carcinomas, suggesting that they have very early lesions in common but take different paths of further development.


Subject(s)
Carcinoma/genetics , Chromosome Aberrations , Ovarian Neoplasms/genetics , Adenoma/genetics , Adult , Aged , Aged, 80 and over , Chromosomes, Human, Pair 12 , Chromosomes, Human, Pair 13 , Chromosomes, Human, Pair 16 , Chromosomes, Human, Pair 20 , Chromosomes, Human, Pair 3 , Chromosomes, Human, Pair 4 , Chromosomes, Human, Pair 6 , Chromosomes, Human, Pair 7 , Chromosomes, Human, Pair 8 , Cystadenocarcinoma, Serous/genetics , Cystadenoma, Serous/genetics , Female , Humans , Middle Aged , Nucleic Acid Hybridization
5.
Virchows Arch ; 440(2): 187-94, 2002 Feb.
Article in English | MEDLINE | ID: mdl-11964050

ABSTRACT

Comparative genomic hybridization (CGH) was used to screen 54 advanced colon carcinomas. i.e., 24 primary tumors and 30 metastases, for chromosomal alterations. Using a sensitive statistical method for the determination of DNA imbalances and histograms for analysis of the incidence of changes, we identified the DNA over-representation of chromosome 20q as the most common alteration being present in 100% of cases. High incidence deletions were observed on 18q21-18q23 (96%), 4q27-4q28 (96%), 4p14 (87%), 5q21 (81%), 1p21-1p22 (72%), 21q21 (74%), 6q16 (72%), 3p12 (66%), 8p24-8p21 (66%), 9p21 (64%), 11q22 (64%), and 14q13-14q21 (64%). Further frequent over-representation was found on 7q12-7q11.2 (75%), 16p11-16p12 (70%), 19p13 (70%), 9q34 (67%), 19q13 (67%), 13q34 (64%), 13q13 (64%), 17q21 (59%), 22q11 (61%), 8q24 (57%), and 1q21 (57%). Pronounced DNA gains and losses being defined as regions in which the ratio profiles exceeded the values of 1.5 and 0.5, respectively, frequently colocalized with peaks of incidence curve. The use of difference histograms for the comparison of tumor subgroups as well as case-by-case histogram for the analysis of 15 paired tumor samples identified several of the above alterations as relevant for tumor progression and metastasis formation. The study identified additional loci and delineates more precisely those that have been previously reported. For comparative purposes, we have made our primary data (ratio profiles, clinicopathological parameters, histograms) available at the interactive web site http://amba.charite.de/cgh, where the incidence of changes can be determined at individual loci and additional parameters can be applied for the analysis of our CGH results.


Subject(s)
Carcinoma/genetics , Chromosome Aberrations , Colorectal Neoplasms/genetics , Neoplasm Metastasis/genetics , Carcinoma/secondary , Colorectal Neoplasms/pathology , Cytogenetic Analysis , DNA, Neoplasm/analysis , Gene Deletion , Genetic Markers , Humans , Neoplasm Metastasis/pathology , Nucleic Acid Hybridization
6.
Science ; 296(5569): 922-7, 2002 May 03.
Article in English | MEDLINE | ID: mdl-11934988

ABSTRACT

Higher order chromatin structure presents a barrier to the recognition and repair of DNA damage. Double-strand breaks (DSBs) induce histone H2AX phosphorylation, which is associated with the recruitment of repair factors to damaged DNA. To help clarify the physiological role of H2AX, we targeted H2AX in mice. Although H2AX is not essential for irradiation-induced cell-cycle checkpoints, H2AX-/- mice were radiation sensitive, growth retarded, and immune deficient, and mutant males were infertile. These pleiotropic phenotypes were associated with chromosomal instability, repair defects, and impaired recruitment of Nbs1, 53bp1, and Brca1, but not Rad51, to irradiation-induced foci. Thus, H2AX is critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA.


Subject(s)
Chromosome Aberrations , DNA Repair , Histones/genetics , Histones/physiology , Recombination, Genetic , Amino Acid Sequence , Animals , B-Lymphocytes/immunology , B-Lymphocytes/physiology , Base Sequence , Cell Cycle , Cells, Cultured , Cellular Senescence , DNA Damage , Female , Gene Targeting , Histones/chemistry , Immunoglobulin Class Switching , Infertility, Male/genetics , Infertility, Male/physiopathology , Lymphocyte Count , Male , Meiosis , Mice , Mice, Knockout , Molecular Sequence Data , Mutation , Phosphorylation , Spermatocytes/physiology , T-Lymphocytes/immunology , T-Lymphocytes/physiology
7.
Cancer Lett ; 176(1): 81-91, 2002 Feb 08.
Article in English | MEDLINE | ID: mdl-11790457

ABSTRACT

Maintenance of telomeres, most often by telomerase, is a necessary prerequisite for immortality of eukaryotic cells. To better understand the mechanisms of telomerase up-regulation during tumorigenesis, we analysed the gene dosage of hTERT on chromosome 5p15, a region known to be overrepresented in a variety of malignancies, in 20 lung cancer cell lines by Southern blotting, fluorescence in-situ hybridization, and comparative genomic hybridization. We found a significant correlation between hTERT gene dosage, hTERT mRNA expression and telomerase activity. Imbalances of chromosome 5p may exert functionally relevant hTERT gene dosage effects in human lung cancer.


Subject(s)
Gene Dosage , Lung Neoplasms/enzymology , Lung Neoplasms/genetics , Telomerase/biosynthesis , Telomerase/genetics , Telomerase/metabolism , Blotting, Southern , Chromosomes, Human, Pair 5 , DNA, Complementary/metabolism , DNA-Binding Proteins , Humans , In Situ Hybridization, Fluorescence , Nucleic Acid Hybridization , RNA, Messenger/metabolism , Tumor Cells, Cultured , Up-Regulation
8.
J Pathol ; 196(1): 8-16, 2002 Jan.
Article in English | MEDLINE | ID: mdl-11748636

ABSTRACT

Comparative genomic hybridization (CGH) was used to screen 83 lung adenocarcinomas of 60 patients for chromosomal imbalances. The most common alteration was DNA overrepresentation on chromosome 1q, with a peak incidence at 1q22-q23 in 73% of the primary tumours, followed by DNA overrepresentation on chromosomes 8q and 20q, and deletions on chromosomes 3p, 4q, 6q, 9p, 9q, and 13q, in at least 60%. The generation of a difference histogram of metastasizing versus non-metastasizing tumours and a case-by-case histogram for the comparison of 23 paired samples of primary tumours and corresponding metastases suggested that deletions on chromosomes 3p12-p14, 3p22-p24, 4p13-15.1, 4q21-qter, 6q21-qter, 8p, 10q, 14q21, 17p12-p13, 20p12, and 21q, and overrepresentations on chromosomes 1q21-q25, 7q11.2, 9q34, 11q12-q13, 14q11-q13, and 17q25 are associated with the metastatic phenotype. In contrast, losses on chromosome 19 and gains on 3p, 4q, 5p, and 6q were preferentially found in non-metastasizing tumours. The analysis of the paired samples revealed considerable chromosomal instability, but indicated a clonal relationship in each case. The primary tumours often showed additional deletions, suggesting that loss of function mutations are critical in the initial phase of tumour dissemination, whereas the metastases preferentially acquired DNA gains, probably modulating the metastatic phenotype. The primary data from this study (ratio profiles, clinicopathological parameters, histograms) are also available at http://amba.charite.de/cgh.


Subject(s)
Adenocarcinoma/genetics , Adenocarcinoma/secondary , Chromosome Aberrations , Lung Neoplasms/genetics , Adenocarcinoma/pathology , Chromosome Deletion , DNA, Neoplasm/genetics , Disease Progression , Female , Follow-Up Studies , Humans , Lung Neoplasms/pathology , Male , Nucleic Acid Hybridization
9.
Nature ; 414(6864): 660-665, 2001 Dec 06.
Article in English | MEDLINE | ID: mdl-11740565

ABSTRACT

Class switch recombination (CSR) is a region-specific DNA recombination reaction that replaces one immunoglobulin heavy-chain constant region (Ch) gene with another. This enables a single variable (V) region gene to be used in conjunction with different downstream Ch genes, each having a unique biological activity. The molecular mechanisms that mediate CSR have not been defined, but activation-induced cytidine deaminase (AID), a putative RNA-editing enzyme, is required for this reaction. Here we report that the Nijmegen breakage syndrome protein (Nbs1) and phosphorylated H2A histone family member X (gamma-H2AX, also known as gamma-H2afx), which facilitate DNA double-strand break (DSB) repair, form nuclear foci at the Ch region in the G1 phase of the cell cycle in cells undergoing CSR, and that switching is impaired in H2AX-/- mice. Localization of Nbs1 and gamma-H2AX to the Igh locus during CSR is dependent on AID. In addition, AID is required for induction of switch region (S mu)-specific DNA lesions that precede CSR. These results place AID function upstream of the DNA modifications that initiate CSR.


Subject(s)
Cytidine Deaminase/physiology , Histones/physiology , Immunoglobulin Class Switching/physiology , Mutagenesis , Nuclear Proteins/physiology , Animals , B-Lymphocytes/immunology , B-Lymphocytes/physiology , BRCA1 Protein/physiology , Base Sequence , Cell Cycle , Cells, Cultured , Cloning, Molecular , Cytidine Deaminase/genetics , DNA , DNA Repair , DNA-Binding Proteins/physiology , Immunoglobulin Heavy Chains/genetics , Lymphocyte Activation , Mice , Mice, Inbred C57BL , Molecular Sequence Data , Rad51 Recombinase , Recombination, Genetic
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