ABSTRACT
To examine the validity of the Children's Form of the Picture-Frustration (P-F) Study, the instrument was administered to 245 children in Grades 1 through 6. Children were also administered the Make A Picture Story (MAPS) and the Response Hierarchy Measure. Teachers rated children's aggression on the Conners Teacher Rating Scale. Hostile responses on the P-F Study were related to self-reported verbal and physical aggression in conflict situations but were unrelated to teachers' ratings of aggressive behavior. Hostile responses were exhibited more often by boys than girls. Responses on the measure were related to children's fantasy patterns. Results supported the validity of the P-F Study as a measure of children's thought content in response to frustration.
Subject(s)
Child Behavior Disorders/diagnosis , Fantasy , Gender Identity , Projective Techniques/statistics & numerical data , Aggression/psychology , Child , Child Behavior Disorders/psychology , Defense Mechanisms , Female , Humans , Male , PsychometricsABSTRACT
This study examined whether the procedure for combining scores to make aggression categories on the Children's Form of the Picture-Frustration Study is psychometrically sound. Subjects were 245 children from Grades 1 through 6. Their responses were rated according to the 11 kinds of responses defined in the manual (Rosenzweig, 1978c). Results indicated that most of the scores did not correlate with their corresponding categories. Thus, the way scores are combined into aggression categories may be invalid, because they seem to be combinations of very dissimilar kinds of responses to frustration. Until psychometrically sound and empirically validated ways of combining the 11 scores are determined, it is recommended that the 11 scores themselves be used for clinical and research purposes.
Subject(s)
Aggression/psychology , Personality Development , Projective Techniques , Child , Female , Humans , Male , PsychometricsSubject(s)
Personality Development , Projective Techniques , Social Conformity , Child , Female , Humans , Male , PsychometricsABSTRACT
Two-dimensional echocardiography has been shown to be a reliable diagnostic procedure in patients with valvular lesions due to fungal endocarditis. We describe a patient with four-chamber Aspergillus endocarditis localized to the nonvalvular endocardium. In this case, two-dimensional echocardiography failed to demonstrate cardiac abnormalities despite the presence of extensive endomyocardial disease at autopsy. The case emphasizes the diagnostic limitations of two-dimensional echocardiography in the absence of valvular vegetations.
Subject(s)
Aspergillosis/diagnosis , Echocardiography/methods , Endocarditis/diagnosis , Adult , Aspergillosis/pathology , Endocarditis/pathology , False Negative Reactions , Humans , Male , Myocardium/pathologySubject(s)
Caffeine/pharmacology , Chromosome Aberrations , Lasers , PUVA Therapy , Photochemotherapy , Animals , Cell Division , Cell Line , Cricetinae , Cricetulus , Female , Interphase , Laser Therapy , Microscopy, Phase-Contrast , OvaryABSTRACT
The light-activated, nucleic acid-binding drugs, psoralens, were used in conjunction with a 365-nm laser microbeam to selectively bind to any nucleic acids in the centriolar region. 4'-aminomethyl-4,5',8--trimethyl-psoralen (AMT) has a high affinity for both RNA and DNA and can be shown to cause mitotic abortion when centriolar regions of prophase PTK2 cells and reacted with AMT and 365-nm laser light. Other psoralen derivatives which have a high affinity for DNA and a low affinity for RNA are not effective in blocking mitosis in dividing PTK2 cells. Examination of psoralen-bound centriolar regions by single-cell electron microscopy shows that at various times after treatment, the number of microtubules associated with the irradiated poles is much lower than in normal, dividing cells. Light-activated psoralen binding of the centriolar regions does not seem to affect the condensation or structure of mitotic chromosomes. It is concluded that there is an RNA in the centriolar region that is responsible for the formation of the spindle in dividing cells.
Subject(s)
Centrioles , Mitosis , Organoids , RNA/physiology , Cells, Cultured , Centrioles/radiation effects , Centrioles/ultrastructure , DNA , Furocoumarins/pharmacology , Lasers , Microscopy, Electron , Microtubules/ultrastructure , Organoids/radiation effects , Organoids/ultrastructureABSTRACT
Potorous tridactylis (PTK2) cells growing in culture were treated with psoralen derivatives and dividing cells were located by phase-contrast microscopy. Psoralens, light-sensitive DNA-photoadducting drugs, were reacted with mitotic chromosomes through exposure to 365-nm light from an argon laser microbeam system. It was found that following mitosis and photoreaction, cells without nuclear envelopes were produced when psoralen-treated cells received 60 light pulses over their entire chromosome complement. These 'non-nuclear membrane' cells were found to incorporate [3H]uridine and, to a lesser extent, [3H]thymidine by autoradiography. Reduction of the light exposure by half (30 near-u.v. pulses) over the entire chromosome complement in the presence of psoralen also produced non-nuclear-membrane cells as seen by light microscopy. Further examination of these cells (30 light pulses) by single-cell electron microscopy revealed that unlike the high light exposure (60 near-u.v. pulses), the low light dosage resulted in cells with membrane patches associated with their chromatin. Since neither actinomycin D nor cycloheximide impeded nuclear envelope reformation, the psoralen-DNA reaction is concluded to produce non-nuclear-membrane cells by a mechanism other than transcription or translation inhibition. The association of Golgi with areas of nuclear membrane patches gives indirect evidence of a possible Golgi contribution to the reformation of the nuclear envelope after mitosis. It is concluded that DNA plays a role in envelope reformation.
