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1.
Chromosoma ; 133(2): 117-134, 2024 Apr.
Article in English | MEDLINE | ID: mdl-38165460

ABSTRACT

Chromosomes with two centromeres provide a unique opportunity to study chromosome breakage and DNA repair using completely endogenous cellular machinery. Using a conditional transcriptional promoter to control the second centromere, we are able to activate the dicentric chromosome and follow the appearance of DNA repair products. We find that the rate of appearance of DNA repair products resulting from homology-based mechanisms exceeds the expected rate based on their limited centromere homology (340 bp) and distance from one another (up to 46.3 kb). In order to identify whether DNA breaks originate in the centromere, we introduced 12 single-nucleotide polymorphisms (SNPs) into one of the centromeres. Analysis of the distribution of SNPs in the recombinant centromeres reveals that recombination was initiated with about equal frequency within the conserved centromere DNA elements CDEII and CDEIII of the two centromeres. The conversion tracts range from about 50 bp to the full length of the homology between the two centromeres (340 bp). Breakage and repair events within and between the centromeres can account for the efficiency and distribution of DNA repair products. We propose that in addition to providing a site for kinetochore assembly, the centromere may be a point of stress relief in the face of genomic perturbations.


Subject(s)
Centromere , Chromosome Breakage , DNA Repair , Centromere/genetics , Animals , Polymorphism, Single Nucleotide , Humans
2.
Nucleic Acids Res ; 52(2): 690-707, 2024 Jan 25.
Article in English | MEDLINE | ID: mdl-37994724

ABSTRACT

Although fusions between the centromeres of different human chromosomes have been observed cytologically in cancer cells, since the centromeres are long arrays of satellite sequences, the details of these fusions have been difficult to investigate. We developed methods of detecting recombination within the centromeres of the yeast Saccharomyces cerevisiae (intercentromere recombination). These events occur at similar rates (about 10-8/cell division) between two active or two inactive centromeres. We mapped the breakpoints of most of the recombination events to a region of 43 base pairs of uninterrupted homology between the two centromeres. By whole-genome DNA sequencing, we showed that most (>90%) of the events occur by non-reciprocal recombination (gene conversion/break-induced replication). We also found that intercentromere recombination can involve non-homologous chromosome, generating whole-arm translocations. In addition, intercentromere recombination is associated with very frequent chromosome missegregation. These observations support the conclusion that intercentromere recombination generally has negative genetic consequences.


Subject(s)
Centromere , Chromosomes, Fungal , Recombination, Genetic , Saccharomyces cerevisiae , Humans , Centromere/genetics , DNA , Saccharomyces cerevisiae/genetics , Translocation, Genetic , Genetic Techniques
3.
PLoS Genet ; 19(1): e1010590, 2023 01.
Article in English | MEDLINE | ID: mdl-36701275

ABSTRACT

Although homologous recombination between transposable elements can drive genomic evolution in yeast by facilitating chromosomal rearrangements, the details of the underlying mechanisms are not fully clarified. In the genome of the yeast Saccharomyces cerevisiae, the most common class of transposon is the retrotransposon Ty1. Here, we explored how Cas9-induced double-strand breaks (DSBs) directed to Ty1 elements produce genomic alterations in this yeast species. Following Cas9 induction, we observed a significant elevation of chromosome rearrangements such as deletions, duplications and translocations. In addition, we found elevated rates of mitotic recombination, resulting in loss of heterozygosity. Using Southern analysis coupled with short- and long-read DNA sequencing, we revealed important features of recombination induced in retrotransposons. Almost all of the chromosomal rearrangements reflect the repair of DSBs at Ty1 elements by non-allelic homologous recombination; clustered Ty elements were hotspots for chromosome rearrangements. In contrast, a large proportion (about three-fourths) of the allelic mitotic recombination events have breakpoints in unique sequences. Our analysis suggests that some of the latter events reflect extensive processing of the broken ends produced in the Ty element that extend into unique sequences resulting in break-induced replication. Finally, we found that haploid and diploid strain have different preferences for the pathways used to repair double-stranded DNA breaks. Our findings demonstrate the importance of DNA lesions in retrotransposons in driving genome evolution.


Subject(s)
CRISPR-Cas Systems , Saccharomyces cerevisiae , Humans , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , CRISPR-Cas Systems/genetics , DNA Breaks, Double-Stranded , Retroelements/genetics , Chromosome Aberrations , Homologous Recombination/genetics
4.
Nucleic Acids Res ; 50(12): 6890-6902, 2022 07 08.
Article in English | MEDLINE | ID: mdl-35748861

ABSTRACT

Ribonucleotides can be incorporated into DNA during replication by the replicative DNA polymerases. These aberrant DNA subunits are efficiently recognized and removed by Ribonucleotide Excision Repair, which is initiated by the heterotrimeric enzyme RNase H2. While RNase H2 is essential in higher eukaryotes, the yeast Saccharomyces cerevisiae can survive without RNase H2 enzyme, although the genome undergoes mutation, recombination and other genome instability events at an increased rate. Although RNase H2 can be considered as a protector of the genome from the deleterious events that can ensue from recognition and removal of embedded ribonucleotides, under conditions of high ribonucleotide incorporation and retention in the genome in a RNase H2-negative strain, sudden introduction of active RNase H2 causes massive DNA breaks and genome instability in a condition which we term 'ribodysgenesis'. The DNA breaks and genome instability arise solely from RNase H2 cleavage directed to the ribonucleotide-containing genome. Survivors of ribodysgenesis have massive loss of heterozygosity events stemming from recombinogenic lesions on the ribonucleotide-containing DNA, with increases of over 1000X from wild-type. DNA breaks are produced over one to two divisions and subsequently cells adapt to RNase H2 and ribonucleotides in the genome and grow with normal levels of genome instability.


Subject(s)
Ribonucleases , Saccharomyces cerevisiae , Humans , Saccharomyces cerevisiae/genetics , Ribonucleases/genetics , Ribonucleotides/genetics , Genomic Instability/genetics , DNA
5.
Proc Natl Acad Sci U S A ; 119(12): e2119588119, 2022 03 22.
Article in English | MEDLINE | ID: mdl-35290114

ABSTRACT

SignificanceAlthough most studies of the genetic regulation of genome stability involve an analysis of mutations within the coding sequences of genes required for DNA replication or DNA repair, recent studies in yeast show that reduced levels of wild-type enzymes can also produce a mutator phenotype. By whole-genome sequencing and other methods, we find that reduced levels of the wild-type DNA polymerase ε in yeast greatly increase the rates of mitotic recombination, aneuploidy, and single-base mutations. The observed pattern of genome instability is different from those observed in yeast strains with reduced levels of the other replicative DNA polymerases, Pol α and Pol δ. These observations are relevant to our understanding of cancer and other diseases associated with genetic instability.


Subject(s)
DNA Polymerase II , Saccharomyces cerevisiae , DNA Polymerase II/metabolism , DNA Replication/genetics , Genomic Instability/genetics , Humans , Mutation , Saccharomyces cerevisiae/metabolism
6.
Curr Opin Genet Dev ; 71: 78-85, 2021 12.
Article in English | MEDLINE | ID: mdl-34311384

ABSTRACT

Saccharomyces cerevisiae is at the forefront of defining the major recombination mechanisms/models that repair targeted double-strand breaks during mitosis. Each of these models predicts specific molecular intermediates as well as genetic outcomes. Recent use of single-nucleotide polymorphisms to track the exchange of sequences in recombination products has provided an unprecedented level of detail about the corresponding intermediates and the extents to which different mechanisms are utilized. This approach also has revealed complexities that are not predicted by canonical models, suggesting that modifications to these models are needed. Current data are consistent with the initiation of most inter-homolog spontaneous mitotic recombination events by a double-strand break. In addition, the sister chromatid is preferred over the homolog as a repair template.


Subject(s)
DNA Breaks, Double-Stranded , Saccharomyces cerevisiae , DNA Repair/genetics , Mitosis/genetics , Saccharomyces cerevisiae/genetics , Sister Chromatid Exchange
7.
Nucleic Acids Res ; 49(10): 5623-5636, 2021 06 04.
Article in English | MEDLINE | ID: mdl-34019669

ABSTRACT

Iron-sulfur clusters (4Fe-4S) exist in many enzymes concerned with DNA replication and repair. The contribution of these clusters to enzymatic activity is not fully understood. We identified the MET18 (MMS19) gene of Saccharomyces cerevisiae as a strong mutator on GC-rich genes. Met18p is required for the efficient insertion of iron-sulfur clusters into various proteins. met18 mutants have an elevated rate of deletions between short flanking repeats, consistent with increased DNA polymerase slippage. This phenotype is very similar to that observed in mutants of POL3 (encoding the catalytic subunit of Pol Î´) that weaken binding of the iron-sulfur cluster. Comparable mutants of POL2 (Pol ϵ) do not elevate deletions. Further support for the conclusion that met18 strains result in impaired DNA synthesis by Pol Î´ are the observations that Pol Î´ isolated from met18 strains has less bound iron and is less processive in vitro than the wild-type holoenzyme.


Subject(s)
DNA Polymerase III/metabolism , DNA Repair , DNA Replication , Iron-Sulfur Proteins/metabolism , Saccharomyces cerevisiae Proteins/metabolism , Saccharomyces cerevisiae/genetics , Transcription Factors/metabolism , Catalytic Domain , DNA-Directed DNA Polymerase/metabolism , Protein Binding
8.
Proc Natl Acad Sci U S A ; 117(45): 28191-28200, 2020 11 10.
Article in English | MEDLINE | ID: mdl-33106417

ABSTRACT

Genomic alterations including single-base mutations, deletions and duplications, translocations, mitotic recombination events, and chromosome aneuploidy generate genetic diversity. We examined the rates of all of these genetic changes in a diploid strain of Saccharomyces cerevisiae by whole-genome sequencing of many independent isolates (n = 93) subcloned about 100 times in unstressed growth conditions. The most common alterations were point mutations and small (<100 bp) insertion/deletions (n = 1,337) and mitotic recombination events (n = 1,215). The diploid cells of most eukaryotes are heterozygous for many single-nucleotide polymorphisms (SNPs). During mitotic cell divisions, recombination can produce derivatives of these cells that have become homozygous for the polymorphisms, termed loss-of-heterozygosity (LOH) events. LOH events can change the phenotype of the cells and contribute to tumor formation in humans. We observed two types of LOH events: interstitial events (conversions) resulting in a short LOH tract (usually less than 15 kb) and terminal events (mostly cross-overs) in which the LOH tract extends to the end of the chromosome. These two types of LOH events had different distributions, suggesting that they may have initiated by different mechanisms. Based on our results, we present a method of calculating the probability of an LOH event for individual SNPs located throughout the genome. We also identified several hotspots for chromosomal rearrangements (large deletions and duplications). Our results provide insights into the relative importance of different types of genetic alterations produced during vegetative growth.


Subject(s)
Chromosomes, Fungal/genetics , Mutation/genetics , Saccharomyces cerevisiae/genetics , Chromosome Mapping , Diploidy , Gene Conversion/genetics , Gene Rearrangement/genetics , Loss of Heterozygosity/genetics , Phenotype , Polymorphism, Single Nucleotide/genetics , Saccharomyces cerevisiae/cytology
9.
Proc Natl Acad Sci U S A ; 117(17): 9440-9450, 2020 04 28.
Article in English | MEDLINE | ID: mdl-32277034

ABSTRACT

Yeast strains with low levels of the replicative DNA polymerases (alpha, delta, and epsilon) have high levels of chromosome deletions, duplications, and translocations. By examining the patterns of mutations induced in strains with low levels of DNA polymerase by the human protein APOBEC3B (a protein that deaminates cytosine in single-stranded DNA), we show dramatically elevated amounts of single-stranded DNA relative to a wild-type strain. During DNA replication, one strand (defined as the leading strand) is replicated processively by DNA polymerase epsilon and the other (the lagging strand) is replicated as short fragments initiated by DNA polymerase alpha and extended by DNA polymerase delta. In the low DNA polymerase alpha and delta strains, the APOBEC-induced mutations are concentrated on the lagging-strand template, whereas in the low DNA polymerase epsilon strain, mutations occur on the leading- and lagging-strand templates with similar frequencies. In addition, for most genes, the transcribed strand is mutagenized more frequently than the nontranscribed strand. Lastly, some of the APOBEC-induced clusters in strains with low levels of DNA polymerase alpha or delta are greater than 10 kb in length.


Subject(s)
Cytidine Deaminase/pharmacology , DNA-Directed DNA Polymerase/metabolism , Fungal Proteins/metabolism , Minor Histocompatibility Antigens/pharmacology , Saccharomyces cerevisiae/genetics , Chromosomes, Fungal , DNA Replication , DNA, Fungal , DNA-Directed DNA Polymerase/genetics , Fungal Proteins/genetics , Gene Expression Regulation, Fungal/physiology , Genome-Wide Association Study , Humans , Mutation , Sequence Analysis, DNA/methods
10.
Aging (Albany NY) ; 11(23): 11686-11721, 2019 12 08.
Article in English | MEDLINE | ID: mdl-31812950

ABSTRACT

Topoisomerase II (Top2) removes topological linkages between replicated chromosomes. Top2 inhibition leads to mitotic catastrophe (MC) when cells unsuccessfully try to split their genetic material between the two daughter cells. Herein, we have characterized the fate of these daughter cells in the budding yeast. Clonogenic and microcolony experiments, in combination with vital and apoptotic stains, showed that 75% of daughter cells become senescent in the short term; they are unable to divide but remain alive. Decline in cell vitality then occurred, yet slowly, uncoordinatedly when comparing pairs of daughters, and independently of the cell death mediator Mca1/Yca1. Furthermore, we showed that senescence can be modulated by ploidy, suggesting that gross chromosome imbalances during segregation may account for this phenotype. Indeed, we found that diploid long-term survivors of the MC are prone to genomic imbalances such as trisomies, uniparental disomies and terminal loss of heterozygosity (LOH), the latter affecting the longest chromosome arms.


Subject(s)
DNA Topoisomerases, Type II/metabolism , Gene Expression Regulation, Enzymologic/physiology , Gene Expression Regulation, Fungal/physiology , Saccharomyces cerevisiae/enzymology , Cell Survival , DNA Topoisomerases, Type II/genetics , Mitosis , Mutation , Saccharomyces cerevisiae/genetics , Single-Cell Analysis
12.
Microb Cell ; 6(1): 1-64, 2019 Jan 07.
Article in English | MEDLINE | ID: mdl-30652105

ABSTRACT

Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.

13.
Nucleic Acids Res ; 47(7): 3521-3535, 2019 04 23.
Article in English | MEDLINE | ID: mdl-30668788

ABSTRACT

Oxidative DNA damage is a threat to genome stability. Using a genetic system in yeast that allows detection of mitotic recombination, we found that the frequency of crossovers is greatly elevated when cells are treated with hydrogen peroxide (H2O2). Using a combination of microarray analysis and genomic sequencing, we mapped the breakpoints of mitotic recombination events and other chromosome rearrangements at a resolution of about 1 kb. Gene conversions and crossovers were the two most common types of events, but we also observed deletions, duplications, and chromosome aneuploidy. In addition, H2O2-treated cells had elevated rates of point mutations (particularly A to T/T to A and C to G/G to C transversions) and small insertions/deletions (in/dels). In cells that underwent multiple rounds of H2O2 treatments, we identified a genetic alteration that resulted in improved H2O2 tolerance by amplification of the CTT1 gene that encodes cytosolic catalase T. Lastly, we showed that cells grown in the absence of oxygen have reduced levels of recombination. This study provided multiple novel insights into how oxidative stress affects genomic instability and phenotypic evolution in aerobic cells.


Subject(s)
Catalase/genetics , DNA Damage/drug effects , Gene Conversion/genetics , Oxidative Stress/drug effects , Chromosomes, Fungal/genetics , Cytosol/enzymology , DNA Repair/drug effects , DNA Replication/drug effects , Genome, Fungal/drug effects , Genomic Instability/drug effects , Hydrogen Peroxide/pharmacology , Mitosis/genetics , Point Mutation/genetics , Saccharomyces cerevisiae/genetics
14.
Genes (Basel) ; 9(11)2018 Nov 07.
Article in English | MEDLINE | ID: mdl-30405078

ABSTRACT

Most cells of solid tumors have very high levels of genome instability of several different types, including deletions, duplications, translocations, and aneuploidy. Much of this instability appears induced by DNA replication stress. As a model for understanding this type of instability, we have examined genome instability in yeast strains that have low levels of two of the replicative DNA polymerases: DNA polymerase α and DNA polymerase δ (Polα and Polδ). We show that low levels of either of these DNA polymerases results in greatly elevated levels of mitotic recombination, chromosome rearrangements, and deletions/duplications. The spectrum of events in the two types of strains, however, differs in a variety of ways. For example, a reduced level of Polδ elevates single-base alterations and small deletions considerably more than a reduced level of Polα. In this review, we will summarize the methods used to monitor genome instability in yeast, and how this analysis contributes to understanding the linkage between genome instability and DNA replication stress.

15.
Proc Natl Acad Sci U S A ; 115(30): E7109-E7118, 2018 07 24.
Article in English | MEDLINE | ID: mdl-29987035

ABSTRACT

The chromosomes of many eukaryotes have regions of high GC content interspersed with regions of low GC content. In the yeast Saccharomyces cerevisiae, high-GC regions are often associated with high levels of meiotic recombination. In this study, we constructed URA3 genes that differ substantially in their base composition [URA3-AT (31% GC), URA3-WT (43% GC), and URA3-GC (63% GC)] but encode proteins with the same amino acid sequence. The strain with URA3-GC had an approximately sevenfold elevated rate of ura3 mutations compared with the strains with URA3-WT or URA3-AT About half of these mutations were single-base substitutions and were dependent on the error-prone DNA polymerase ζ. About 30% were deletions or duplications between short (5-10 base) direct repeats resulting from DNA polymerase slippage. The URA3-GC gene also had elevated rates of meiotic and mitotic recombination relative to the URA3-AT or URA3-WT genes. Thus, base composition has a substantial effect on the basic parameters of genome stability and evolution.


Subject(s)
Base Composition , Base Sequence , Recombination, Genetic , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae/genetics , Sequence Deletion , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/metabolism
16.
mBio ; 8(6)2017 12 19.
Article in English | MEDLINE | ID: mdl-29259092

ABSTRACT

Although meiosis in warm-blooded organisms takes place in a narrow temperature range, meiosis in many organisms occurs over a wide variety of temperatures. We analyzed the properties of meiosis in the yeast Saccharomyces cerevisiae in cells sporulated at 14°C, 30°C, or 37°C. Using comparative-genomic-hybridization microarrays, we examined the distribution of Spo11-generated meiosis-specific double-stranded DNA breaks throughout the genome. Although there were between 300 and 400 regions of the genome with high levels of recombination (hot spots) observed at each temperature, only about 20% of these hot spots were found to have occurred independently of the temperature. In S. cerevisiae, regions near the telomeres and centromeres tend to have low levels of meiotic recombination. This tendency was observed in cells sporulated at 14°C and 30°C, but not at 37°C. Thus, the temperature of sporulation in yeast affects some global property of chromosome structure relevant to meiotic recombination. Using single-nucleotide polymorphism (SNP)-specific whole-genome microarrays, we also examined crossovers and their associated gene conversion events as well as gene conversion events that were unassociated with crossovers in all four spores of tetrads obtained by sporulation of diploids at 14°C, 30°C, or 37°C. Although tetrads from cells sporulated at 30°C had slightly (20%) more crossovers than those derived from cells sporulated at the other two temperatures, spore viability was good at all three temperatures. Thus, despite temperature-induced variation in the genetic maps, yeast cells produce viable haploid products at a wide variety of sporulation temperatures.IMPORTANCE In the yeast Saccharomyces cerevisiae, recombination is usually studied in cells that undergo meiosis at 25°C or 30°C. In a genome-wide analysis, we showed that the locations of genomic regions with high and low levels of meiotic recombination (hot spots and cold spots, respectively) differed dramatically in cells sporulated at 14°C, 30°C, and 37°C. Thus, in yeast, and likely in other non-warm-blooded organisms, genetic maps are strongly affected by the environment.


Subject(s)
Meiosis/radiation effects , Recombination, Genetic/radiation effects , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/radiation effects , Temperature , Comparative Genomic Hybridization , DNA Breaks, Double-Stranded , Microarray Analysis , Microbial Viability/radiation effects , Saccharomyces cerevisiae/growth & development , Spores, Fungal/genetics , Spores, Fungal/growth & development , Spores, Fungal/radiation effects
17.
Genome Res ; 27(12): 2072-2082, 2017 12.
Article in English | MEDLINE | ID: mdl-29113982

ABSTRACT

Improper DNA double-strand break (DSB) repair results in complex genomic rearrangements (CGRs) in many cancers and various congenital disorders in humans. Trinucleotide repeat sequences, such as (GAA)n repeats in Friedreich's ataxia, (CTG)n repeats in myotonic dystrophy, and (CGG)n repeats in fragile X syndrome, are also subject to double-strand breaks within the repetitive tract followed by DNA repair. Mapping the outcomes of CGRs is important for understanding their causes and potential phenotypic effects. However, high-resolution mapping of CGRs has traditionally been a laborious and highly skilled process. Recent advances in long-read DNA sequencing technologies, specifically Nanopore sequencing, have made possible the rapid identification of CGRs with single base pair resolution. Here, we have used whole-genome Nanopore sequencing to characterize several CGRs that originated from naturally occurring DSBs at (GAA)n microsatellites in Saccharomyces cerevisiae These data gave us important insights into the mechanisms of DSB repair leading to CGRs.


Subject(s)
DNA Breaks, Double-Stranded , DNA Repair , DNA, Fungal , Gene Rearrangement , Nanopores , Saccharomyces cerevisiae/genetics , Sequence Analysis, DNA/methods , Chromosomes, Fungal , Gene Duplication , Genome, Fungal , Retroelements , Trinucleotide Repeats
18.
Elife ; 62017 07 17.
Article in English | MEDLINE | ID: mdl-28714850

ABSTRACT

In yeast, DNA breaks are usually repaired by homologous recombination (HR). An early step for HR pathways is formation of a heteroduplex, in which a single-strand from the broken DNA molecule pairs with a strand derived from an intact DNA molecule. If the two strands of DNA are not identical, there will be mismatches within the heteroduplex DNA (hetDNA). In wild-type strains, these mismatches are repaired by the mismatch repair (MMR) system, producing a gene conversion event. In strains lacking MMR, the mismatches persist. Most previous studies involving hetDNA formed during mitotic recombination were restricted to one locus. Below, we present a global mapping of hetDNA formed in the MMR-defective mlh1 strain. We find that many recombination events are associated with repair of double-stranded DNA gaps and/or involve Mlh1-independent mismatch repair. Many of our events are not explicable by the simplest form of the double-strand break repair model of recombination.


Subject(s)
DNA, Fungal/genetics , Mitosis/radiation effects , Nucleic Acid Heteroduplexes/analysis , Recombination, Genetic , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/radiation effects , Ultraviolet Rays , Brain Neoplasms , Colorectal Neoplasms , MutL Protein Homolog 1/deficiency , Neoplastic Syndromes, Hereditary , Saccharomyces cerevisiae/physiology , Saccharomyces cerevisiae Proteins
19.
Genetics ; 206(2): 785-800, 2017 06.
Article in English | MEDLINE | ID: mdl-28381587

ABSTRACT

In the yeast Saccharomyces cerevisiae, the genes encoding the metallothionein protein Cup1 are located in a tandem array on chromosome VIII. Using a diploid strain that is heterozygous for an insertion of a selectable marker (URA3) within this tandem array, and heterozygous for markers flanking the array, we measured interhomolog recombination and intra/sister chromatid exchange in the CUP1 locus. The rate of intra/sister chromatid recombination exceeded the rate of interhomolog recombination by >10-fold. Loss of the Rad51 and Rad52 proteins, required for most interhomolog recombination, led to a relatively small reduction of recombination in the CUP1 array. Although interhomolog mitotic recombination in the CUP1 locus is elevated relative to the average genomic region, we found that interhomolog meiotic recombination in the array is reduced compared to most regions. Lastly, we showed that high levels of copper (previously shown to elevate CUP1 transcription) lead to a substantial elevation in rate of both interhomolog and intra/sister chromatid recombination in the CUP1 array; recombination events that delete the URA3 insertion from the CUP1 array occur at a rate of >10-3/division in unselected cells. This rate is almost three orders of magnitude higher than observed for mitotic recombination events involving single-copy genes. In summary, our study shows that some of the basic properties of recombination differ considerably between single-copy and tandemly-repeated genes.


Subject(s)
Homologous Recombination/genetics , Metallothionein/genetics , Recombination, Genetic , Saccharomyces cerevisiae Proteins/genetics , Meiosis/genetics , Mitosis/genetics , Multigene Family/genetics , Rad51 Recombinase/genetics , Rad52 DNA Repair and Recombination Protein/genetics , Saccharomyces cerevisiae/genetics , Sister Chromatid Exchange , Tandem Repeat Sequences
20.
Proc Natl Acad Sci U S A ; 113(50): E8114-E8121, 2016 12 13.
Article in English | MEDLINE | ID: mdl-27911848

ABSTRACT

DNA replication stress (DRS)-induced genomic instability is an important factor driving cancer development. To understand the mechanisms of DRS-associated genomic instability, we measured the rates of genomic alterations throughout the genome in a yeast strain with lowered expression of the replicative DNA polymerase δ. By a genetic test, we showed that most recombinogenic DNA lesions were introduced during S or G2 phase, presumably as a consequence of broken replication forks. We observed a high rate of chromosome loss, likely reflecting a reduced capacity of the low-polymerase strains to repair double-stranded DNA breaks (DSBs). We also observed a high frequency of deletion events within tandemly repeated genes such as the ribosomal RNA genes. By whole-genome sequencing, we found that low levels of DNA polymerase δ elevated mutation rates, both single-base mutations and small insertions/deletions. Finally, we showed that cells with low levels of DNA polymerase δ tended to accumulate small promoter mutations that increased the expression of this polymerase. These deletions conferred a selective growth advantage to cells, demonstrating that DRS can be one factor driving phenotypic evolution.


Subject(s)
DNA Replication/genetics , DNA, Fungal/genetics , DNA, Fungal/metabolism , Genomic Instability , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Aneuploidy , Chromosomes, Fungal/genetics , DNA Copy Number Variations , DNA Polymerase III/metabolism , Humans , INDEL Mutation , Loss of Heterozygosity , Neoplasms/genetics , Oligonucleotide Array Sequence Analysis , Point Mutation , Polymorphism, Single Nucleotide , Saccharomyces cerevisiae Proteins/metabolism , Sequence Analysis, DNA , Stress, Physiological , Tandem Repeat Sequences
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