1.
J Chromatogr
; 500: 585-93, 1990 Feb 02.
Article
in English
| MEDLINE
| ID: mdl-2329151
ABSTRACT
A biospecific sorbent for the isolation of ovalbumin antibodies was prepared by coupling of ovalbumin via its periodate-oxidized carbohydrate moiety to bead cellulose modified with adipic acid dihydrazide. The anti-ovalbumin IgG fraction isolated on this sorbent from immune rabbit serum contained only antibodies against protein determinants of ovalbumin. Thus, when these IgG were immobilized through their carbohydrate moieties to cellulose beads it became possible to prepare a biospecific sorbent for concanavalin A by oriented adsorption of ovalbumin. Ovalbumin was specifically adsorbed via its protein moiety and its carbohydrate part remained free for interaction with concanavalin A.
Subject(s)
Antibodies/isolation & purification , Carbohydrates , Concanavalin A/isolation & purification , Immunoglobulin G/isolation & purification , Ovalbumin , Adsorption , Animals , Chromatography, Affinity , Concanavalin A/analysis , Immune Sera , Male , Ovalbumin/immunology , Ovalbumin/isolation & purification , Rabbits
2.
Akush Ginekol (Sofiia)
; 16(3): 210-3, 1977.
Article
in Bulgarian
| MEDLINE
| ID: mdl-900426
3.
Nahrung
; 15(3): 289-94, 1971.
Article
in German
| MEDLINE
| ID: mdl-5172902