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1.
Int J Parasitol ; 31(5-6): 499-511, 2001 May 01.
Article in English | MEDLINE | ID: mdl-11334935

ABSTRACT

Infection with Trypanosoma cruzi causes a generalised vasculitis of several vascular beds. This vasculopathy is manifested by vasospasm, reduced blood flow, focal ischaemia, platelet thrombi, increased platelet aggregation and elevated plasma levels of thromboxane A(2) and endothelin-1. In the myocardium of infected mice, myonecrosis and a vasculitis of the aorta, coronary artery, smaller myocardial vessels and the endocardial endothelium are observed. Immunohistochemistry studies employing anti-endothelin-1 antibody revealed increased expression of endothelin-1, most intense in the endocardial and vascular endothelium. Elevated levels of mRNA for prepro endothelin-1, endothelin converting enzyme and endothelin-1 were observed in the infected myocardium. When T. cruzi-infected mice were treated with phosphoramidon, an inhibitor of endothelin converting enzyme, there was a decrease in heart size and severity of pathology. Mitogen-activated protein kinases and the transcription factor activator-protein-1 regulate the expression of endothelin-1. Therefore, we examined the activation of mitogen-activated protein kinases in the myocardium by T. cruzi. Western blot demonstrated an extracellular signal regulated kinase. In addition, the activator-protein-1 DNA binding activity, as determined by electrophoretic mobility shift assay, was increased. Increased expression of cyclins A and cyclin D1 was observed in the myocardium, and immunohistochemistry studies revealed that interstitial cells and vascular and endocardial endothelial cells stained intensely with antibodies to these cyclins. These data demonstrate that T. cruzi infection of the myocardium activates extracellular signal regulated kinase, activator-protein-1, endothelin-1, and cyclins. The activation of these pathways is likely to contribute to the pathogenesis of chagasic heart disease. These experimental observations suggest that the vasculature plays a role in the pathogenesis of chagasic cardiomyopathy. Additionally, the identification of these pathways provides possible targets for therapeutic interventions to ameliorate or prevent the development of cardiomyopathy during T. cruzi infection.


Subject(s)
Chagas Disease/etiology , Endothelin-1/physiology , Gene Expression Regulation/physiology , Animals , Chagas Cardiomyopathy/etiology , Chagas Cardiomyopathy/parasitology , Chagas Cardiomyopathy/physiopathology , Chagas Disease/parasitology , Chagas Disease/physiopathology , Endothelin-1/biosynthesis , Endothelin-1/blood , Heart/parasitology , Humans , Immunohistochemistry , Mice
2.
Immunology ; 103(1): 122-9, 2001 May.
Article in English | MEDLINE | ID: mdl-11380700

ABSTRACT

During acute Trypanosoma cruzi infection in mice, many leucocytes undergo apoptosis. Although apoptosis has been ascribed to increased levels of nitric oxide (NO) and Fas-FasL interaction, the importance of this phenomenon in modulating the host response against T. cruzi is unknown. Herein, the role of NO- and Fas-FasL-induced apoptosis in modulating the immune response to T. cruzi was evaluated using mice deficient in Fas expression (MRL/MpJ-Fas lpr) and inducible nitric oxide synthase (iNOS) knockout mice (iNOS-/-). The results showed that besides decreasing apoptosis induction after infection, impairment of the Fas-FasL interaction resulted in decreased NO production, as a consequence of enhanced T helper 2 (Th2) cytokine production. Differently, blockage of NO-induced apoptosis resulted in uncontrolled cytokine production, rather than a biased Th2 cytokine pattern. Together, these results suggested that Fas and FasL-induced apoptosis could be implied in modulation of the immune response against T. cruzi by interfering with cytokine and NO production during the acute phase of the infection.


Subject(s)
Chagas Disease/immunology , Membrane Glycoproteins/metabolism , Nitric Oxide/biosynthesis , fas Receptor/metabolism , Acute Disease , Animals , Apoptosis/immunology , Cell Culture Techniques , Chagas Disease/metabolism , Chagas Disease/pathology , Cytokines/biosynthesis , Disease Susceptibility , Fas Ligand Protein , Ligands , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Mice, Knockout
3.
J Cardiovasc Pharmacol ; 36(5 Suppl 1): S148-50, 2000 Nov.
Article in English | MEDLINE | ID: mdl-11078362

ABSTRACT

Chagas' disease, caused by the parasite Trypanosoma cruzi, is an important cause of heart disease. Previous studies from this laboratory revealed that microvascular spasm and myocardial ischemia were observed in infected mice. Infection of endothelial cells with this parasite increased the synthesis of biologically active endothelin-1 (ET-1). Therefore. in the myocardium of T. cruzi-infected mice, we examined ET-1 expression and the p42/44-mitogen activated protein kinase (MAPK)-AP-1 pathway that regulates the expression of ET-1. There was parasitism and myonecrosis in the myocardium of infected C57BL/6 mice. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis revealed elevated mRNA expression of transcription factor AP-1 (c-jun and c-fos) and increased AP-1 DNA binding activity as determined by electrophoretic mobility shift assay (EMSA). Western blot analysis demonstrated an increase in the phosphorylated forms of extracellular signal-regulated kinase (ERK1/2). ET-1 mRNA was upregulated in the myocardium of infected mice. Immunohistochemical and immunoelectron microscopy using anti-ET-1 antibody detected increased expression in cardiac myocytes and endothelium of these mice. These data suggest that ET-1 contributes to chagasic cardiomyopathy and that the mechanism of the increased expression of ET-1 is a result of the activation of the MAPK pathway by T. cruzi infection.


Subject(s)
Chagas Disease/metabolism , Endothelin-1/genetics , MAP Kinase Signaling System , Myocardium/metabolism , Animals , Enzyme Activation , Male , Mice , Mice, Inbred C57BL , Mitogen-Activated Protein Kinases/metabolism , RNA, Messenger/analysis , Transcription Factor AP-1/metabolism
4.
Cardiovasc Pathol ; 9(5): 257-65, 2000.
Article in English | MEDLINE | ID: mdl-11064272

ABSTRACT

Chagas' disease, caused by Trypanosoma cruzi, is an important cause of myocarditis and chronic cardiomyopathy and is accompanied by microvascular spasm and myocardial ischemia. We reported previously that infection of cultured endothelial cells with T. cruzi increased the synthesis of biologically active endothlein-1 (ET-1). In the present study, we examined the role of ET-1 in the cardiovascular system of CD1 mice infected with the Brazil strain of T. cruzi and C57BL/6 mice infected with the Tulahuen strain during acute infection. In the myocardium of infected mice myonecrosis and multiple pseudocysts were observed. There was also an intense vasculitis of the aorta, coronary artery, smaller myocardial vessels and the endocardial endothelium. Immunohistochemistry studies employing anti-ET-1 antibody revealed increased expression of ET-1 that was most intense in the endocardial and vascular endothelium. Elevated levels of mRNA for preproET-1, endothelin converting enzyme and ET-1 were observed in the same myocardial samples. Plasma ET-1 levels were significantly elevated in infected CD1 mice 10-15 days post infection. These observations suggest that increased levels of ET-1 are a consequence of the initial invasion of the cardiovascular system and provide a mechanism for infection-associated myocardial dysfunction.


Subject(s)
Chagas Cardiomyopathy/metabolism , Endothelin-1/metabolism , Myocardium/metabolism , Trypanosoma cruzi/isolation & purification , Animals , Aspartic Acid Endopeptidases/genetics , Aspartic Acid Endopeptidases/metabolism , Biomarkers , Chagas Cardiomyopathy/parasitology , Coronary Vessels/parasitology , Coronary Vessels/pathology , DNA Primers/chemistry , Endothelin-1/genetics , Endothelin-Converting Enzymes , Endothelins/genetics , Endothelins/metabolism , Endothelium, Vascular/parasitology , Endothelium, Vascular/pathology , Male , Metalloendopeptidases/genetics , Metalloendopeptidases/metabolism , Mice , Mice, Inbred C57BL , Myocardial Ischemia/metabolism , Myocardial Ischemia/parasitology , Myocarditis/metabolism , Myocarditis/parasitology , Protein Precursors/genetics , Protein Precursors/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction
5.
Front Biosci ; 5: D452-60, 2000 Apr 01.
Article in English | MEDLINE | ID: mdl-10762598

ABSTRACT

Injury to the cardiovascular system causes an elevated expression of endothelin-1 (ET-1) and activation of several important signaling pathways including the mitogen-activated kinase (MAPK) cascade. The activation of these pathways has been implicated in the pathogenesis of cardiovascular disease caused by hypoxia, infections, and ischemia /reperfusion injury, cardiomyopathy and restenosis after balloon angioplasty. Important downstream targets of the MAPK and ET-1 pathways are the cell cycle regulatory molecules (cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors). Regulation of these molecules contributes to remodeling throughout the cardiovascular system. In addition, cell cycle molecules are important in the regulation of angiogenesis. These new data have led to the development of potential therapeutic modalities targeting these regulatory molecules in order to ameliorate various cardiovascular disease states.


Subject(s)
Cardiovascular Diseases/metabolism , Cell Cycle Proteins/metabolism , Endothelins/metabolism , Cardiovascular Diseases/pathology , Cell Cycle/physiology , Endothelium, Vascular/physiology , Humans , Mitogen-Activated Protein Kinases/metabolism
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