ABSTRACT
Surface chemistry of CoCr particles is demonstrated to be fundamental to the process of phagocytosis by fibroblast cells in vitro. Particles preincubated in serum for 5 days and washed in water before addition to cell cultures were phagocytosed less readily than were particles preincubated in minimal essential medium (MEM) for 1 h and washed in water. This was explained by the coating of calcium phosphate and protein on the serum-immersed particles investigated by time-of-flight secondary ion mass spectroscopy. The cells incubated with the serum-immersed particles had a reduced mitotic index when compared with the MEM-immersed particles, indicating that the phagocytosed particles were causing cell cycle arrest. The release of soluble ions measured by electrothermal atomic absorption spectroscopy within the first hour of particle immersion in MEM was identified as the most likely cause for the DNA damage measured by single cell gel electrophoresis ("Comet" assay). Cryofocused ion beam SEM with a spatial resolution of 8 nm was used to cross section cells, to investigate the location of the phagocytosed particles, some of which were found within the nuclear membrane. This paper demonstrated that consideration of the surface chemistry is essential to understand the processes of the effects of orthopedic wear debris.
Subject(s)
Biocompatible Materials/chemistry , Biocompatible Materials/toxicity , Chromium Alloys/chemistry , Chromium Alloys/toxicity , DNA Damage , Phagocytosis , Cell Cycle/drug effects , Cells, Cultured , Fibroblasts/cytology , Fibroblasts/drug effects , Fibroblasts/physiology , Humans , Materials Testing , Microscopy, Electron, Scanning , Prosthesis Failure , Spectrometry, Mass, Secondary Ion , Surface PropertiesABSTRACT
The inhibitory effect of synthetic elastase inhibitor was studied in experimental emphysema. The development of emphysema was totally prevented by this substance. According to the authors' opinion the administration of synthetic elastase inhibitors can be considered as a new possibility in the treatment of pulmonary emphysema.
Subject(s)
Amino Acid Chloromethyl Ketones/therapeutic use , Pancreatic Elastase/antagonists & inhibitors , Pulmonary Emphysema/prevention & control , Aerosols , Amino Acid Chloromethyl Ketones/administration & dosage , Animals , RatsABSTRACT
The effect of a synthetic elastase inhibitor was studied in experimental emphysema. The development of emphysema was totally prevented by this material. In the authors' opinion the administration of synthetic elastase inhibitors can be considered as a new possibility in the treatment of pulmonary emphysema.
Subject(s)
Amino Acid Chloromethyl Ketones/pharmacology , Pancreatic Elastase/antagonists & inhibitors , Pulmonary Emphysema/prevention & control , Aerosols , Amino Acid Chloromethyl Ketones/therapeutic use , Animals , Lung/pathology , Male , Pancreatic Elastase/toxicity , Pulmonary Emphysema/chemically induced , RatsABSTRACT
Pulmonary emphysema was induced in rats by leuko-elastase extracted from purulent sputum, with the aim of throwing light on the part played by the elastolytic activity in the pathogenesis of pulmonary emphysema. A brief review of the pertaining literature is presented.
