ABSTRACT
The composition and the antimicrobial activity of the aerial parts of Hypericum rumeliacum are reported. Analysis was carried out by GC/MS. The major constituents were alpha-pinene (43.80%), beta-pinene (9.82%), dehydro-aromadendrene (6.81%) and alpha-copaene (5.41%). The essential oil showed a moderate in vitro activity against the six Gram negative and positive bacteria and a stronger one against the three-tested pathogenic activity.
Subject(s)
Anti-Bacterial Agents/pharmacology , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Hypericum , Phytotherapy , Plant Oils/pharmacology , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/therapeutic use , Bicyclic Monoterpenes , Bridged Bicyclo Compounds/administration & dosage , Bridged Bicyclo Compounds/chemistry , Bridged Bicyclo Compounds/pharmacology , Bridged Bicyclo Compounds/therapeutic use , Gas Chromatography-Mass Spectrometry , Humans , Microbial Sensitivity Tests , Monoterpenes/administration & dosage , Monoterpenes/chemistry , Monoterpenes/pharmacology , Monoterpenes/therapeutic use , Plant Oils/administration & dosage , Plant Oils/chemistry , Plant Oils/therapeutic use , Plant StemsABSTRACT
Terpene composition of needle and cortical oleoresin from lateral shoots were analyzed by GC/MS for four Silver fir (Abies alba Mill.) populations scattered in natural species range in Albania. More than sixty compounds were detected in the needle oleoresin, which was characterized by a high content of alpha-pinene, camphene, beta-pinene, limonene and bornyl acetate. Three monoterpenes, alpha-pinene, beta-pinene and limonene, and two sesquiterpenes, beta-caryophyllene and germacrene D, comprised the majority of cortical oleoresin. The terpene composition differences among the populations that led to the recognition of two chemotypes. The needle oleoresin from the provinces of Puka, Bulqiza and Llogara were characterised by high amounts of beta-pinene, camphene and alpha-pinene and low amounts of limonene, while that from Drenova had high amounts of beta-pinene and limonene. A similar pattern was found in the cortical oleoresin with the exception of camphene that was a minor contributor. Geographical and seasonal variation between the populations was, also, investigated. Multivariate analysis of both needle and cortical oleoresin separated Drenova (southeastern population) from the other sites. When both major monoterpenes and sesquiterpenes were considered four chemical profiles could be attributed. Based on their chemical profiles, the populations can be divided into two groups: Populations with high content of beta-pinene and alpha-pinene but a low content of limonene (Puka, Bulqiza and Llogara), typical of most of A. alba populations in all its distribution range. Population with a high content of limonene and a moderate content of beta-pinene and alpha-pinene (Drenova).
