ABSTRACT
Using the Western blot method, the authors analyzed 85 sera obtained from patients with rheumatic diseases, focused on the presence of antihistones and antiactin autoantibodies. The authors detected a 32% incidence of the two investigated autoantibody specificities. In a group of 42 patients with systemic lupus erythematosus in 22 sera (52%) positive antihistone antibodies were present, whereby autoantibodies anti-H1 and anti-H2B were most frequent. In 15 sera in this group of patients (36%) antiactin autoantibodies were present.
Subject(s)
Actins/immunology , Autoantibodies/analysis , Collagen Diseases/immunology , Histones/immunology , Antibody Specificity , Blotting, Western , HumansABSTRACT
The authors describe a case of primary Sjögren's syndrome, which was complicated with severe autoimmune agranulocytosis quite sensitive to immunosuppressive therapy. Agranulocytosis is a very rare complication of this autoimmune rheumatic disease as opposed to leucopenia. A remarkable feature of the presented case is the fact that correct diagnosis of primary Sjögren's syndrome has not been settled for almost 25 years. The disease has manifested only in the form of arthropathy imitating rheumatoid arthritis.
Subject(s)
Agranulocytosis/complications , Sjogren's Syndrome/complications , Agranulocytosis/blood , Agranulocytosis/drug therapy , Autoimmune Diseases/blood , Autoimmune Diseases/complications , Autoimmune Diseases/drug therapy , Cyclophosphamide/therapeutic use , Female , Humans , Immunosuppressive Agents/therapeutic use , Middle Aged , Prednisone/therapeutic use , Sjogren's Syndrome/physiopathologyABSTRACT
Using the Western blot method, the authors analyzed 85 sera obtained from patients with rheumatic diseases, focused on the presence of antihistones and antiactin autoantibodies. The authors detected a 32% incidence of the two investigated autoantibody specificities. In a group of 42 patients with systemic lupus erythematosus in 22 sera (52%) positive antihistone antibodies were present, whereby autoantibodies anti-H1 and anti-H2B were most frequent. In 15 sera in this group of patients (36%) antiactin autoantibodies were present.
Subject(s)
Actins/immunology , Autoantibodies/analysis , Histones/immunology , Rheumatic Diseases/immunology , Blotting, Western , HumansABSTRACT
The immunolocalization of cyclin/PCNA in synchronized 3T3 cells was performed with human autoantibodies using an immunogold technique performed on thin cryosections. Previous immunofluorescent studies demonstrated that the DNA replication sites correspond to the localization of bound cyclin. We have found that in the early periods of S phase, the DNA replication sites (or sites potentially ready for the replication during the hydroxyurea DNA synthesis block) are situated in the perichromatin region and correspond to clustered gold particles present frequently over a morphologically distinct small nuclear area. Heavily labeled chromocenters, including perinucleolar condensed chromatin, exhibiting several such distinct areas were found in later periods of S phase.
Subject(s)
Cell Nucleus/metabolism , DNA Replication , Nuclear Proteins/metabolism , Animals , Cell Compartmentation , Cell Line , Cell Nucleus/ultrastructure , DNA Replication/drug effects , Hydroxyurea/pharmacology , Immunohistochemistry , Mice , Proliferating Cell Nuclear AntigenABSTRACT
In a two-year retrospective study the incidence of diabetes was investigated in 481 patients with systemic rheumatic disease. The control group was formed by 120 patients with degenerative articular disease of the lower extremities. The authors found a significantly higher incidence of diabetes mellitus in both groups, as compared with the population of the Czech Socialist Republic, whereby in patients with systemic rheumatic disease probably the relatively frequent administration of glucocorticoids plays a part. The remarkably high incidence of insulin-dependent diabetes in 16% of patients with polymyositis might suggest a possible co-existence of these two autoimmune diseases.
