The effect of solution pH on the structural stability of magnetoferritin.

The structural stability of magnetoferritin, a synthetic analogue of ferritin, at various pH levels is assessed here. The structural and electrical properties of the complexes were determined by small-angle X-ray scattering (SAXS), dynamic light scattering (DLS) and zeta potential measurements. At pH 3-6 a reduction of electrostatic repulsion on the suspended colloids resulted in aggregation and sedimentation of magnetoferritin. At neutral to slightly alkaline conditions (pH 7-9) the magnetoferritin structure was stable for lower iron loadings. Higher solution pH 10-12 induced destabilization of the protein structure and dissociation of subunits. Increasing the loading factor in the MFer complex leads to decrease of the stability versus pH changes.

Structural self-organization of C60 and cisplatin in physiological solution.

The specific features of structural self-organization of C60 fullerene and antitumor drug cisplatin (Cis) in physiological solution (0.9% NaCl) have been investigated by means of small-angle neutron scattering, scanning electron and atomic force microscopies, as well as isothermal titration calorimetry, dynamic light scattering and UV-Vis spectroscopy. The formation of C60 + Cis complexes, has been reported, unveiling the mechanism of medico-biological synergy observed during administration of the mixture of these drugs.

Effect of iron oxide loading on magnetoferritin structure in solution as revealed by SAXS and SANS.

Synthetic biological macromolecule of magnetoferritin containing an iron oxide core inside a protein shell (apoferritin) is prepared with different content of iron. Its structure in aqueous solution is analysed by small-angle synchrotron X-ray (SAXS) and neutron (SANS) scattering. The loading factor (LF) defined as the average number of iron atoms per protein is varied up to LF=800. With an increase of the LF, the scattering curves exhibit a relative increase in the total scattered intensity, a partial smearing and a shift of the match point in the SANS contrast variation data. The analysis shows an increase in the polydispersity of the proteins and a corresponding effective increase in the relative content of magnetic material against the protein moiety of the shell with the LF growth. At LFs above ∼150, the apoferritin shell undergoes structural changes, which is strongly indicative of the fact that the shell stability is affected by iron oxide presence.

On the origin of C60 fullerene solubility in aqueous solution.

In this work, we report that the surface hydroxylation of C60 molecules is the most likely mechanism for pristine C60 fullerenes/C60 fullerene aggregate stabilization in water, being independent of the method of C60 fullerene aqueous solution preparation.

[PARTICULARITIES OF CARBOHYDRATE METABOLISM IN PATIENTS WITH PULMONARY TUBERCULOSIS WITH PRECLINICAL DISORDERS GLICEMIA].

Studied concentration of lactate and pyruvate in patiens with lung tuberculosis with disorders of tolerance to glucosis and glukemic disorders on an empty stomach and in patiens, wich had not such disorders. In patiens with preclinical disorders glicemia discovered increasing to concentrations of lactate and pyruvate in blood. This is indicative of more deep breaches of quality to oxidation the glucose in energetic aerobic reaction, in particular, in tricarboxylic acids cycle. Effect this is a deterioration energy status of the cells. Excess of the formation lactate and pyruvate on background of the effect anty-insulin hormone prevalence and relative insufficiency of the insulin realizes the independent contribution to development hyperglicemia disorders.

[Treatment of drug resistant destructive pulmonary tuberculosis: gemifloxacin and other fluoroquinolones clinical efficiency and tolerance at the end of initial phase of treatment].

Gemifloxacin efficiency and tolerance in comparison to the ofloxacin, levofloxacin and gatifloxacin during the intensive phase of the antituberculosis therapy for drug resistant cases was evaluated. 156 drug resistant TB patients were examined in the open, prospective, randomized research, being divided into 2 groups with similar drug resistance profile. The 1st group received gemifloxacin, the 2nd--other fluoroquinolones. Gemifloxacin efficiency in the treatment regimen for the drug resistant TB patients did not differ from the efficiency of the use of other fluoroquinolones of the 4th generation and was significantly higher in comparison to ofloxacin. At the same time the identical level of side effects was registered in the course of treatment with mentioned drugs. Gemifloxacin is effective and safe at treatment of tuberculosis in comparison to other fluoroquinolones that allows considering it as the drug of choice among fluoroquinolones for treatment of drug resistant TB, including multidrug-resistant TB.

Spontaneus productions of interleukins in patients with the first diagnosed pulmonary tuberculosis.

The authors showed in the article an analysis related to the level of spontaneous products of IL-1, IL-2, IL-4, IL-6, IL-12, gamma-IFN in blood serum of patients with first diagnosed pulmonary tuberculosis in comparison with healthy donors who have a negative and hyper reaction to a Mantous skin test. It was found out the decrease in the level of gamma-IFN in patients with tuberculosis of lungs and healthy patients with a hyper reaction to a Mantous skin test. The lowest production of IL-1 was found out in patients with local tuberculosis, the greatest - in patients with infiltrative lung tuberculosis. The authors have not found out dependence of the level of probed interleukin on the form of tuberculosis process in lungs.

[The prostacyclin-thromboxane system and bronchial patency in patients with pulmonary tuberculosis]. / Sostoianie prostatsiklin-tromboksanovoi sistemy i bronkhial'noi prokhodimosti u bol'nykh tuberkulezom legkikh.

The paper is concerned with a study in which a contribution was assessed of the prostacyclin-thromboxane system to the development of disturbances in bronchial patency in patients with pulmonary tuberculosis. It has been established that the prostacyclin-thromboxane system has an active part to play in the evolution and formation both the pattern of the tuberculous process and ventilatory disorders, the most important contribution being made by the proportion prostacyclin:thromboxane.

[The clinico-pathogenetic role of the eicosanoid system in pulmonary tuberculosis]. / Kliniko-patogeneticheskaia rol' sistemy éikozanoidov pri tuberkuleze legkikh.

Contents of leukotrienes B4, C4, C4/D4/E4, prostaglandins E and F2a, thromboxane B2 and 6-keto-PgF1a were studied by radioimmunoassay in patients with fresh and chronic pulmonary tuberculosis. Eicosanoids were established to take an active part in pathogenetical mechanisms of tuberculous process. Local forms of the disease were characterized by moderately risen level both of leukotrienes and prostaglandins. Freshly detected disseminated destructive process was accompanied by increased activity mainly of LtC4, LtC4/D4/E4 as well as of prostacyclin-thromboxane system and PgF2a. Advance of pulmonary tuberculosis was associated with activated LtB4 and TxB2 synthesis and decreased production of PgI2, PgE, LtC4, LtC4/D4/E4.

[The results of pneumothermometry and mucociliary clearance study in pulmonary tuberculosis patients]. / Rezul'taty pnevmotermometrii i issledovaniia mikotsiliarnogo klirensa u bol'nykh tuberkulezom legkikh.

Pneumothermometry was carried out in 136 patients with different forms of pulmonary tuberculosis. It was found that the temperature of the alveolar air in pulmonary tuberculosis patients depended on the character of the specific process on a lesser degree than on the spread of the process. The temperature of the exhaled alveolar air was most reduced in patients suffering of spread pulmonary tuberculosis with marked fibrosis of the pulmonary tissue. Disorders of the drainage function of the bronchi reflected mostly the character of the specific lesion of the pulmonary tissue and was most pronounced in patients with fibrous-cavernous cirrhotic tuberculosis.