ABSTRACT
A 49-year-old patient with changes in the nails of the hallux for 10 years was diagnosed with onychomycosis. The identity of the causative agent was confirmed as Cladosporium halotolerans from the Cladosporium sphaerospermum species complex using molecular techniques. MALDI-TOF identified the agent as C. sphaerospermum complex species. Overall, species such as onychomycosis agents should attract special attention to avoid mistakes in the identification process while considering a probable contaminant as responsible for the disease. These species deserve attention since there are rare descriptions of them as causes of onychomycosis. It is important to recognize them as causes of disease and not just as a probable contaminant.
Subject(s)
Onychomycosis , Humans , Middle Aged , Onychomycosis/drug therapy , Onychomycosis/diagnosisABSTRACT
ABSTRACT A 49-year-old patient with changes in the nails of the hallux for 10 years was diagnosed with onychomycosis. The identity of the causative agent was confirmed as Cladosporium halotolerans from the Cladosporium sphaerospermum species complex using molecular techniques. MALDI-TOF identified the agent as C. sphaerospermum complex species. Overall, species such as onychomycosis agents should attract special attention to avoid mistakes in the identification process while considering a probable contaminant as responsible for the disease. These species deserve attention since there are rare descriptions of them as causes of onychomycosis. It is important to recognize them as causes of disease and not just as a probable contaminant.
ABSTRACT
Sporotrichosis is a polymorphic disease of humans and animals, which is acquired via traumatic inoculation of Sporothrix propagules into cutaneous or subcutaneous tissue. The etiological agents are in a clinical complex, which includes Sporothrix brasiliensis, Sporothrix schenckii, Sporothrix globosa, and Sporothrix luriei, each of which has specific epidemiological and virulence characteristics. Classical manifestation in humans includes a fixed localized lesion at the site of trauma plus lymphocutaneous sporotrichosis with fungal spreading along the lymphatic channels. Atypical sporotrichosis is a challenge to diagnosis because it can mimic many other dermatological diseases. We report an unusual, itraconazole-resistant cutaneous lesion of sporotrichosis in a 66-year-old Brazilian man. Histopathological examination of the skin revealed vascular and fibroblastic proliferation with chronic granulomatous infiltrate composed of multinucleated giant cells. Sporothrix were isolated from the skin lesion, and phylogenetic analyses confirmed it to be sporotrichosis due to S. globosa, a widespread pathogen. Immunoblotting analysis showed several IgG-reactive molecules in autochthonous preparations of the whole cellular proteins (160, 80, 60, 55, 46, 38, 35, and 30 kDa) and exoantigen (35 and 33 kDa). The patient was first unsuccessfully treated with daily itraconazole, and then successfully treated with potassium iodide.
Subject(s)
Antifungal Agents/pharmacology , Drug Resistance, Fungal , Itraconazole/pharmacology , Sporothrix/drug effects , Sporotrichosis/microbiology , Sporotrichosis/pathology , Aged , Humans , Male , Phylogeny , Potassium Iodide/therapeutic use , Sporothrix/genetics , Sporotrichosis/drug therapyABSTRACT
We report four cases of scalp white piedra (SWP) in Brazilian female children. Morphological and physiological approaches gave inconsistent results for identifying Trichosporon to species level, while the sequencing of the intergenic spacer 1 region of ribosomal DNA accurately identified the agent of SWP as T. inkin. These cases emphasize the occurrence of this species causing this type of infection. The molecular identification of the suspected agent is needed for appropriate epidemiological surveillance of superficial mycoses caused by Trichosporon species.
Subject(s)
Piedra/diagnosis , Piedra/microbiology , Scalp/microbiology , Trichosporon/isolation & purification , Trichosporonosis/diagnosis , Trichosporonosis/microbiology , Brazil , Child , DNA, Fungal/chemistry , DNA, Fungal/genetics , DNA, Ribosomal Spacer/chemistry , DNA, Ribosomal Spacer/genetics , Female , Humans , Molecular Sequence Data , Piedra/pathology , Scalp/pathology , Sequence Analysis, DNA , Trichosporonosis/pathologyABSTRACT
OBJECTIVE: The aim of this work was to compare the effects of estradiol and genistein treatment on hyaluronic acid (HA) concentration in postmenopausal facial skin. METHODS: In this study, 30 postmenopausal women were evaluated in a prospective, randomized, double-blind trial. The volunteers were postmenopausal women treated in the Gynecology Department of the Federal University of São Paulo. The participants were divided into two groups: group E, treated with 0.01% 17ß-estradiol gel (n = 15), and group G, treated with 4% genistein gel (isoflavones, n = 15). The treatment lasted for 24 consecutive weeks. Preauricular skin biopsies were performed for each participant at baseline (E1 and G1) and after treatment (E2 and G2) to evaluate HA concentration in tissue. The materials were processed using immunohistochemical and biochemical methods. RESULTS: After 24 weeks of treatment, HA concentration increased in both groups, but the effect was greater for estradiol treatment than for genistein treatment. CONCLUSIONS: Our data suggest that both treatments may enhance HA concentration in postmenopausal skin but that estrogen produces results that are greater than those produced by isoflavones.
Subject(s)
Estradiol/administration & dosage , Genistein/administration & dosage , Hyaluronic Acid/analysis , Postmenopause , Skin/chemistry , Skin/drug effects , Biopsy , Double-Blind Method , Face , Female , Gels , Humans , Immunohistochemistry , Middle Aged , Prospective StudiesABSTRACT
BACKGROUND: Androgenetic alopecia is the most common form of hair loss. It is a clinical entity of relevant interest and presents a significant psychosocial impact as it undermines self-esteem and quality of life in female patients due to the importance of the hair for people's facial balance. OBJECTIVE: The purpose of the present study is to evaluate dermoscopic signs in women clinically diagnosed with androgenetic alopecia. METHOD: Observational study with 34 women between 17 and 68 years old who were diagnosed with androgenetic alopecia. All of them underwent photographic sessions with a 10x magnification dermoscope and a digital camera zoom set to 20x magnification and 40x magnification on the scalp frontal midline. RESULTS: All patients showed miniaturization. A peripilar brown halo was found in 22 patients, honeycomb-like scalp pigmentation was found in 14 and yellow dots in only 1 patient. Recent studies show dermoscopy as the new tool for diagnosis assistance and treatment follow up in scalp disorders. Our study used an ordinary dermoscope and we evaluated several findings reported in the literature with significant clarity and easiness. CONCLUSION: The dermoscope, which is used by dermatologists on a daily basis, is an excellent tool to assist in early diagnosis and assessment of therapeutic response in androgenetic alopecia.
Subject(s)
Alopecia/pathology , Dermoscopy , Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Severity of Illness Index , Young AdultABSTRACT
BACKGROUND: Androgenetic alopecia is the most common form of hair loss. It is a clinical entity of relevant interest and presents a significant psychosocial impact as it undermines self-esteem and quality of life in female patients due to the importance of the hair for people's facial balance. OBJECTIVE: The purpose of the present study is to evaluate dermoscopic signs in women clinically diagnosed with androgenetic alopecia. METHOD: Observational study with 34 women between 17 and 68 years old who were diagnosed with androgenetic alopecia. All of them underwent photographic sessions with a 10x magnification dermoscope and a digital camera zoom set to 20x magnification and 40x magnification on the scalp frontal midline. RESULTS: All patients showed miniaturization. A peripilar brown halo was found in 22 patients, honeycomb-like scalp pigmentation was found in 14 and yellow dots in only 1 patient. Recent studies show dermoscopy as the new tool for diagnosis assistance and treatment follow up in scalp disorders. Our study used an ordinary dermoscope and we evaluated several findings reported in the literature with significant clarity and easiness. CONCLUSION: The dermoscope, which is used by dermatologists on a daily basis, is an excellent tool to assist in early diagnosis and assessment of therapeutic response in androgenetic alopecia.
FUNDAMENTOS: Alopecia androgenética é forma mais comum de queda de cabelo. Constitui entidade clínica de interesse relevante e acarreta grande impacto psicossocial por comprometer a auto-estima e a qualidade de vida das pacientes. OBJETIVO: Procurar os sinais dermatoscópicos comuns em mulheres com diagnóstico clínico de alopecia androgenética, visando diagnóstico precoce e melhor resposta terapêutica. MÉTODOS: Estudo observacional em 34 mulheres com idades entre 17 e 68 anos, com diagnóstico clínico de alopecia androgenética. Todas pacientes foram avaliadas e submetidas a registros fotográficos com o dermatoscópio no aumento de 10x e câmera digital no aumento de 20x e 40x na linha média frontal do couro cabeludo. RESULTADOS: Todas as pacientes apresentaram miniaturização dos fios na área examinada. Halo castanho peripilar foi encontrado em 22 pacientes, pigmentação em favo de mel em 14 e pontos amarelos em apenas 1 paciente. Estudos recentes mostram a dermatoscopia como uma nova ferramenta no auxílio diagnóstico e acompanhamento do tratamento das desordens do couro cabeludo. Em nosso estudo utilizamos um dermatoscópio comum e avaliamos vários achados relatados na literatura com facilidade e clareza significativas. CONCLUSÃO: O dermatoscópio, instrumento de uso diário dos dermatologistas, é excelente ferramenta para auxílio no diagnóstico precoce e avaliação da resposta terapêutica na alopecia androgenética e é capaz de mostrar de forma simples sinais precoces de miniaturização capilar.
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Middle Aged , Young Adult , Alopecia/pathology , Dermoscopy , Severity of Illness IndexABSTRACT
Chronic cutaneous lupus erythematosus is a polymorphous autoimmune disease which may mimic some other clinical conditions, causing diagnostic difficulties. Acneiform lesions, including comedones and pitting scars are occasionally atypical presentations of cutaneous discoid lupus erythematosus.
Subject(s)
Facial Dermatoses/pathology , Lupus Erythematosus, Discoid/pathology , Adult , Anti-Bacterial Agents/therapeutic use , Chronic Disease , Facial Dermatoses/drug therapy , Female , Humans , Hydroxychloroquine/therapeutic use , Lupus Erythematosus, Discoid/drug therapy , Tetracycline/therapeutic useABSTRACT
An 83 year old woman, exhibiting severe psoriasis, was treated conventionally (phototherapy, acitretin, and cyclosporine). After poor clinical results and significant changes in laboratory procedures, those treatments were suspended. She was then being prepared to be submitted to biological treatment, when preliminary results disclosed a 30 mm PPD. Complete improvement occurred [only] after introducing prophylactic therapy for tuberculosis and anti-depressive medication.
Subject(s)
Antidepressive Agents/therapeutic use , Depressive Disorder/drug therapy , Psoriasis/drug therapy , Sertraline/therapeutic use , Aged, 80 and over , Antitubercular Agents/therapeutic use , Depressive Disorder/complications , Female , Humans , Induction Chemotherapy/methods , Isoniazid/therapeutic use , Male , Psoriasis/etiology , Tuberculosis, Cutaneous/drug therapy , Tuberculosis, Cutaneous/etiologyABSTRACT
Lúpus eritematoso cutâneo crônico é doença autoimune, com apresentações polimorfas que podem, eventualmente, mimetizar outras condições clínicas, causando dificuldade diagnóstica. Quadro acneiforme, com comedões e lesões atróficas pontuadas (pitting scars) pode constituir apresentação atípica do lúpus eritematoso cutâneo discóide.
Chronic cutaneous lupus erythematosus is a polymorphous autoimmune disease which may mimic some other clinical conditions, causing diagnostic difficulties. Acneiform lesions, including comedones and pitting scars are occasionally atypical presentations of cutaneous discoid lupus erythematosus.
Subject(s)
Adult , Female , Humans , Facial Dermatoses/pathology , Lupus Erythematosus, Discoid/pathology , Anti-Bacterial Agents/therapeutic use , Chronic Disease , Facial Dermatoses/drug therapy , Hydroxychloroquine/therapeutic use , Lupus Erythematosus, Discoid/drug therapy , Tetracycline/therapeutic useABSTRACT
Mulher idosa apresentou psoríase em placas do tipo grave, com tendência eritrodérmica, e foi submetida a tratamento de acordo com o algoritmo consensual (fototerapia, acitretina, ciclosporina). Resultados clínicos insuficientes, recorrência e agravamento do quadro laboratorial orientaram no sentido da introdução de terapia biológica. A avaliação preliminar revelou PPD de 30mm. A resolução completa das lesões se verificou quando realizada profilaxia antituberculose e administrado antidepressivo.
An 83 year old woman, exhibiting severe psoriasis, was treated conventionally (phototherapy, acitretin, and cyclosporine). After poor clinical results and significant changes in laboratory procedures, those treatments were suspended. She was then being prepared to be submitted to biological treatment, when preliminary results disclosed a 30mm PPD. Complete improvement occurred [only] after introducing prophylactic therapy for tuberculosis and anti-depressive medication.
Subject(s)
Aged, 80 and over , Female , Humans , Male , Antidepressive Agents/therapeutic use , Depressive Disorder/drug therapy , Psoriasis/drug therapy , Sertraline/therapeutic use , Antitubercular Agents/therapeutic use , Depressive Disorder/complications , Induction Chemotherapy/methods , Isoniazid/therapeutic use , Psoriasis/etiology , Tuberculosis, Cutaneous/drug therapy , Tuberculosis, Cutaneous/etiologyABSTRACT
Reports of child abuse have increased significantly. The matter makes most physicians uncomfortable for two reasons: a) Little guidance or no training in recognizing the problem; b - Not understanding its true dimension. The most common form of child violence is physical abuse. The skin is the largest and frequently the most traumatized organ. Bruises and burns are the most visible signs. Physicians (pediatricians, general practitioners and dermatologists) are the first professionals to observe and recognize the signs of intentional injury. Dermatologists particularly, can help distinguish intentional injury from accidental, or from skin diseases that mimic maltreatment.
Subject(s)
Burns/diagnosis , Child Abuse/diagnosis , Contusions/diagnosis , Skin/injuries , Child , Child Abuse, Sexual/diagnosis , Dermatology , Diagnosis, Differential , Female , Humans , MaleABSTRACT
As denúncias de abuso contra a criança têm sido frequentes e configuram grave problema de saúde pública. O tema é desconfortável para muitos médicos, seja pelo treinamento insuficiente, seja pelo desconhecimento das dimensões do problema. Uma das formas mais comuns de violência contra a criança é o abuso físico. Como órgão mais exposto e extenso, a pele é o alvo mais sujeito aos maustratos. Equimoses e queimaduras são os sinais mais visíveis. Médicos (pediatras, clínicos-gerais e dermatologistas) costumam ser os primeiros profissionais a observar e reconhecer sinais de lesões não acidentais ou intencionais. Os dermatologistas podem auxiliar na distinção entre lesões traumáticas intencionais, acidentais e doenças cutâneas que mimetizam maus-tratos.
Reports of child abuse have increased significantly. The matter makes most physicians uncomfortable for two reasons: a) Little guidance or no training in recognizing the problem; b - Not understanding its true dimension. The most common form of child violence is physical abuse. The skin is the largest and frequently the most traumatized organ. Bruises and burns are the most visible signs. Physicians (pediatricians, general practitioners and dermatologists) are the first professionals to observe and recognize the signs of intentional injury. Dermatologists particularly, can help distinguish intentional injury from accidental, or from skin diseases that mimic maltreatment.
Subject(s)
Child , Female , Humans , Male , Burns/diagnosis , Child Abuse/diagnosis , Contusions/diagnosis , Skin/injuries , Child Abuse, Sexual/diagnosis , Dermatology , Diagnosis, DifferentialABSTRACT
We report the case of an atypical disseminated leishmaniasis with similar clinical characteristics to post-kala-azar dermal leishmaniasis, an uncommon disease in South America. This occurred in a Brazilian patient with AIDS, 3 years after the first episode of American visceral leishmaniasis.
Subject(s)
HIV Infections/complications , Leishmania infantum/pathogenicity , Leishmaniasis, Cutaneous/etiology , Leishmaniasis, Visceral/complications , AIDS-Related Opportunistic Infections/drug therapy , AIDS-Related Opportunistic Infections/parasitology , Adult , Animals , Brazil , Diagnosis, Differential , Fatal Outcome , Humans , Leishmania infantum/isolation & purification , Leishmaniasis, Cutaneous/diagnosis , Leishmaniasis, Cutaneous/parasitology , Leishmaniasis, Cutaneous/pathology , Leishmaniasis, Visceral/drug therapy , Leishmaniasis, Visceral/parasitology , MaleABSTRACT
INTRODUCTION: Psoriasis is a debilitating, chronic inflammatory systemic disease affecting around 2% of the South American population. Biological therapies offer the possibility of long-term therapy with improved safety and efficacy. METHODS: We conducted a multicentre, open-label, single-arm, Phase IIIb/IV study of adult patients (18-75 years) with moderate-to-severe plaque psoriasis who were candidates for systemic therapy or phototherapy. Patients received efalizumab subcutaneously (1.0 mg/kg/wk). The primary endpoint was the proportion of patients achieving a Physician Global Assessment (PGA) rating of "excellent" or "cleared" at Week 24. Safety outcomes were adverse events (AEs), serious AEs (SAEs) and abnormalities on laboratory tests. RESULTS: Of 189 patients included in the intent-to-treat and safety populations, 104 (55.0%) were of Hispanic or Latino ethnicity. At Week 24, 92/189 (48.7%) patients achieved or maintained a PGA rating of "excellent" or "cleared". AEs were reported by 161/189 (85.2%) patients, SAEs by 21/189 (11.1%). One patient died during the study (meningoencephalitis). Laboratory findings were consistent with previous experience. CONCLUSIONS: Efalizumab demonstrated sustained control of psoriasis up to 24 weeks in patients from Latin America, confirming results seen in Phase III studies conducted in North America and Europe.
ABSTRACT
OBJECTIVE: To analyze the dermal collagen of 15 postmenopausal women who had being treated with systemic estrogen replacement before and after using topical a 0.01% estrogen treatment. METHODS: Fifteen patients were included in this clinical trial using the systemic estrogen therapy for at least 1 year (minimum and maximum lengths of therapy were 13 and 40 months, respectively). A facial punch was performed in the preauricular area for collecting samples before and after the 16 weeks of treatment. Blood samples were also collected for estradiol level determination. The morphometric determination of epithelial and dermal thickness as well as dermal collagen were measured using a suitable software. The paired Student's t-test was used for statistical analysis. RESULTS: The epithelial and dermal thickness enhanced after the topic estrogen therapy (P<0.01). The amount of collagen significantly increased after 16 weeks of treatment (P<0.001). The estrogen levels did not significant increase after the topical therapy (P > or = 0.05). CONCLUSION: Our data suggested that topical estrogen associated to systemic estrogen therapy seems to increase the expression of skin collagen amount, which may prove to be beneficial for the postmenopausal facial skin.
