Subject(s)
Macrophages/cytology , Mitosis , Peritoneum/cytology , Spleen/cytology , Animals , Culture Techniques , Macrophages/immunology , Mice , Microscopy, Phase-Contrast , Motion Pictures , Phagocytosis , UrodelaSubject(s)
Hydantoins/pharmacology , Inflammation , Leukocytes/drug effects , Macrophages/drug effects , Phagocytosis/drug effects , Adult , Female , Humans , Male , Neutrophils/drug effectsABSTRACT
In vitro culture of rat peritoneal macrophages, cells generally considered devoid of proliferative activity, was found to lead to the appearance of mitosis. Mitotic index and percent phase incidence values were determined for these cells.
Subject(s)
Cell Proliferation , Macrophages, Peritoneal/physiology , Mitosis , Mitotic Index , Animals , Cells, Cultured , Male , RatsABSTRACT
In the course of further studies of mitotic activity in vitro cultures of rat peritoneal macrophages, an increment in proliferative volume between the 2nd and 6th day was observed, followed by a decrease. The relationships between these variations and those of the mitotic index (previously reported) are discussed.
Subject(s)
Cell Proliferation , Macrophages, Peritoneal/physiology , Mitotic Index , Animals , Cells, Cultured , Male , RatsABSTRACT
Contrast phase microscopy was employed to determine mitosis phase duration values in rat peritoneal macrophages in which proliferative activity had appeared following culture in vitro. Mitosis duration values were considerable and tended to increase in function of increases in culture time. These data are discussed in a general account of in vitro reproductive activity in peritoneal macrophages.