ABSTRACT
We studied the expression of insulin-like growth factor 1 (IGF-1), androgen receptor (AR) and luteinizing hormone receptor (LHR) in the ovaries under the conditions of the modeling and subsequent treatment of functional ovarian cysts with gonadotropin-releasing hormone antagonist (ant-GnRH). The intensity of IGF-1, LHR, and AR expression in the generative elements of rat ovaries changed under conditions of functional ovarian cysts simulation, as well as during treatment with ant-GnRH. In both experimental groups, the expression levels of the studied markers in preantral follicles and epithelial lining of cysts were found to be related to the number of growing follicles and cysts. A divergence of LHR and AR expression indices and a more pronounced decrease in the number of cystic cavities were observed in the group receiving ant-GnRH. These changes demonstrate a positive effect of ant-GnRH on intra-ovarian regulatory factors and a therapeutic effect in functional ovarian cysts.
Subject(s)
Cysts , Ovarian Cysts , Female , Rats , Animals , Humans , Receptors, LH , Gonadotropin-Releasing Hormone/pharmacology , Gonadotropin-Releasing Hormone/metabolism , Insulin-Like Growth Factor I/genetics , Insulin-Like Peptides , Receptors, Androgen/genetics , Ovarian Cysts/drug therapyABSTRACT
The morphofunctional features of the ovaries were evaluated in rats with functional ovarian cysts model treated with gonadotropin-releasing hormone antagonist. Administration of the antagonist significantly (p=0.009) reduced the number of cysts and the growth of follicles in the ovaries. The obtained results attest to a possibility of successful treatment of functional ovarian cysts with gonadotropin-releasing hormone antagonist.
Subject(s)
Cysts , Ovarian Cysts , Female , Humans , Rats , Animals , Gonadotropin-Releasing Hormone , Ovarian Cysts/drug therapy , Models, TheoreticalABSTRACT
The levels of anti-Müllerian hormone, follicle-stimulating hormone, and testosterone were studied in rats after removal of the uterine horns, oviducts, or combined surgery. The effects of preventive salpingectomy on ovarian reserve were studied. After preventive salpingectomy, the level of anti-Müllerian hormone was low throughout the experiment, follicle-stimulating hormone increased only by the end of observation (on day 40), and testosterone level decreased only on day 10. Hysterectomy alone caused no changes in the concentrations of the studied hormones on day 10 after surgery. Salpingectomy alone reduced the concentrations of anti-Müllerian hormone and testosterone and did not change the level of follicle-stimulating hormone during this period.
Subject(s)
Fallopian Tubes/surgery , Ovarian Reserve/genetics , Prophylactic Surgical Procedures , Salpingectomy/methods , Uterus/surgery , Animals , Anti-Mullerian Hormone/blood , Anti-Mullerian Hormone/genetics , Fallopian Tubes/metabolism , Female , Follicle Stimulating Hormone/blood , Follicle Stimulating Hormone/genetics , Gene Expression , Hysterectomy/methods , Rats , Rats, Wistar , Testosterone/blood , Testosterone/genetics , Uterus/metabolismABSTRACT
Morphological study of ovarian follicular cysts induced by chorionic gonadotropin (300 U) and insulin (Protafane HM; 2.5 U) in adult rats showed that superovulatory dose of chorionic gonadotropin and moderate hyperinsulinemia induced the development of ovarian cysts on days 3-5 in 100% cases. Dynamic study of ovarian morphology showed that changes were reversible and the cysts regressed within 60 days, which confirmed their functional nature.
Subject(s)
Chorionic Gonadotropin , Insulin , Ovarian Cysts , Ovary , Animals , Chorionic Gonadotropin/metabolism , Chorionic Gonadotropin/pharmacology , Female , Insulin/metabolism , Insulin/pharmacology , Ovarian Cysts/metabolism , Ovarian Cysts/pathology , Ovary/cytology , Ovary/drug effects , RatsABSTRACT
Using the methods of light and electron microscopy, morpho-functional changes of the ovaries of albino rats after their monopolar coagulation were studied. Electrocoagulation was shown to cause hemodynamic changes in a perifocal area and short-term stimulation of follicular growth, but starting already at postoperational day 3, their atresia was increased, while by day 30 a significant reduction in number of both primordial and growing follicles, as well as of corpora lutea, was found. Ultrastructural changes of oocytes in primary and secondary follicles outside the coagulation zone were demonstrated at early stages and included the reduction of porous plate, cortical and yolk granule numbers, and the increase in numbers of lysosomes and multivesicular bodies. Ascovertin, due to its antioxidant properties and by improving hemorheological parameters, exerts a protective action on ovarian generative apparatus and the state of microcirculation in the organ.
Subject(s)
Antioxidants/pharmacology , Ascorbic Acid/pharmacology , Ovary/drug effects , Ovary/ultrastructure , Quercetin/analogs & derivatives , Regeneration/drug effects , Animals , Drug Combinations , Electrocoagulation , Female , Flavonols/pharmacology , Ovary/physiology , Quercetin/pharmacology , RatsABSTRACT
Fragmented embryonic bone tissue stimulates bone regeneration. Bone formation starts not from implanted embryonic fragments, but in intact periosteum and endosteum containing cambial cells of the osteodifferon. In rabbits, recovery of damaged radial bone after implantation of fragmented embryonic bone tissue into bone defect was associated with a pronounced periosteal reaction and focal resorption of intact ulnar bone. Consolidation of damaged radial bone without implantation of fragmented embryonic bone tissue was incomplete in all experimental animals.
