ABSTRACT
Dependence of growth characteristics of cell population and the monolayer formation on the initial plating concentrations of cells were studied on the established CHO cell line. The cells were cultivated under standard conditions on plastic substrate. Initial plating concentration was varied as: 1000, 2000, 3000, 4000, 5000, and 6000 cell/cm2. It was shown that growth of the cell culture can be formally described by standard S-shaped dependence. However, a more detailed analysis revealed a discrepancy between the experimental and expected data. Specifically the arrest of cell growth at the monolayer formation does not coincide with the time when the theoretical curves approach plateau. It has been concluded that cell proliferation and the formation of a monolayer are independent processes (at last in CHO cells). Both processes may be considered as analogues of proliferation and morphogenesis in metazoan. In addition, it has been shown that the arrest of cell division occurs not only by contact inhibition of proliferation during monolayer formation but also by reducing the size of cells to some limiting dimension and by increasing the polarization of cells.
Subject(s)
Cell Division/physiology , Cell Polarity/physiology , Cell Shape/physiology , Animals , CHO Cells , Cricetinae , CricetulusABSTRACT
A procedure has been developed to prepare the anthelminthic Rilanide as a fine-dispersed formulation. The therapeutical dose of the agent in fascioliasis and intestinal nematodiasis is 60 mg/kg (for sheep), if given alone, and 100 mg/kg, if added to feed.
Subject(s)
Anthelmintics/administration & dosage , Anthelmintics/chemical synthesis , Benzene Derivatives/chemical synthesis , Benzene Derivatives/therapeutic use , Fascioliasis/drug therapy , Hydrocarbons, Halogenated/chemical synthesis , Hydrocarbons, Halogenated/therapeutic use , Intestinal Diseases, Parasitic/drug therapy , Nematode Infections/drug therapy , Sheep Diseases/drug therapy , Animals , Anthelmintics/chemistry , Benzene Derivatives/chemistry , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical/veterinary , Fascioliasis/veterinary , Hydrocarbons, Halogenated/chemistry , Intestinal Diseases, Parasitic/veterinary , Nematode Infections/veterinary , SheepABSTRACT
A procedure was developed for the synthesis of the anthelminthic G-1460. The therapeutical doses (20 and 25 mg/kg) of the agent were defined for the treatment of monieziasis and gastrointestinal nematodes on an individual basis.
Subject(s)
Anticestodal Agents/chemical synthesis , Antinematodal Agents/chemical synthesis , Benzene Derivatives/chemical synthesis , Intestinal Diseases, Parasitic/veterinary , Monieziasis/drug therapy , Nematode Infections/veterinary , Sheep Diseases/drug therapy , Animals , Anticestodal Agents/therapeutic use , Antinematodal Agents/therapeutic use , Benzene Derivatives/therapeutic use , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , Intestinal Diseases, Parasitic/drug therapy , Nematode Infections/drug therapy , Niclosamide/therapeutic use , SheepABSTRACT
The study was undertaken to examine the fasciolacidal activity of the agents G-1411, G-1423, and G-1439. Tested, G-1439 was chosen for detailed investigations as a non-toxic fasciolacide.
Subject(s)
Antiplatyhelmintic Agents/administration & dosage , Benzamides/administration & dosage , Fascioliasis/drug therapy , Fascioliasis/veterinary , Sheep Diseases/drug therapy , Animals , Antiplatyhelmintic Agents/toxicity , Benzamides/toxicity , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical/veterinary , Mice , SheepABSTRACT
A procedure has been developed to manufacture the anthelminthic Triclonate. The agent was tested for the treatment of sheep's natural monieziasis infection and it was found to have a high activity.
Subject(s)
Anticestodal Agents/chemical synthesis , Anticestodal Agents/therapeutic use , Animals , Anticestodal Agents/analysis , Anticestodal Agents/toxicity , Drug Evaluation, Preclinical/veterinary , Mice , Monieziasis/drug therapy , Niclosamide/therapeutic use , Sheep , Sheep Diseases/drug therapy , Spectrophotometry, Infrared , Technology, PharmaceuticalABSTRACT
A manufacturing procedure has been developed to prepare the anthelmintic bromoxane as a finely divided dosage form. The maximum toxic dose of the drug was 1 g per kg mice body weight. Its therapeutic dose in sheep fascioliasis was 20 mg/kg.
Subject(s)
Antiplatyhelmintic Agents/chemical synthesis , Benzamides/chemical synthesis , Fascioliasis/drug therapy , Sheep Diseases/drug therapy , Animals , Antiplatyhelmintic Agents/therapeutic use , Antiplatyhelmintic Agents/toxicity , Benzamides/therapeutic use , Benzamides/toxicity , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical/veterinary , Fascioliasis/veterinary , Mice , Sheep , Technology, PharmaceuticalSubject(s)
Antiplatyhelmintic Agents/chemical synthesis , Benzamides/chemical synthesis , Dicrocoeliasis/drug therapy , Fascioliasis/drug therapy , Animals , Antiplatyhelmintic Agents/analysis , Antiplatyhelmintic Agents/therapeutic use , Benzamides/analysis , Benzamides/therapeutic use , Chromatography, Thin Layer , Dicrocoeliasis/veterinary , Drug Evaluation/veterinary , Fascioliasis/veterinary , Sheep , Sheep Diseases/drug therapy , Spectrophotometry, Infrared , Spectrophotometry, UltravioletABSTRACT
The authors describe the technology of preparing a new anthelmintic agent triclazan, N-(3,4-dichlorophenyl)-2-[(benzo-2,1,3-thiadiazole-4-sulfonyl)amino]-5- chlorobenzamide, finely dispersed (the particles size 4-8 mu). Triclazan is highly effective not only in hymenolepiasis and trichocephaliasis, as was shown previously, but in fascioliasis, monieziasis and intestinal nematodiasis of sheep as well.