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1.
Morfologiia ; 148(4): 7-11, 2015.
Article in Russian | MEDLINE | ID: mdl-26601464

ABSTRACT

The study was conducted on 6 female and 36 male adult Wistar rats to compare the effects of dissociated cells derived from different embryonic CNS anlages, on the growth of regenerating nerve fibers in the damaged nerve of the recipient. After the sciatic nerve was damaged by ligation, part of the animals received the injection into the proximal portion of the nerve with a suspension of the cells obtained by dissociation of the fragments of spinal cord or forebrain vesicle taken from rat embryos at Day 15 of development. The analysis of transverse semithin sections of the distal part of the nerves was performed 21 and 60 days after surgery. It was found that the number of regenerating myelinated nerve fibers was increased 60 days after the injection of dissociated embryonic spinal cord cells, but not the neocortical cells into the damaged nerve of the recipient.


Subject(s)
Embryonic Stem Cells/transplantation , Nerve Fibers, Myelinated/physiology , Nerve Regeneration , Neural Stem Cells/transplantation , Peripheral Nerve Injuries/therapy , Animals , Cells, Cultured , Female , Male , Prosencephalon/cytology , Prosencephalon/embryology , Rats , Rats, Wistar , Sciatic Nerve/injuries , Sciatic Nerve/physiology , Spinal Cord/cytology , Spinal Cord/embryology , Transplantation, Homologous
2.
Morfologiia ; 148(6): 64-9, 2015.
Article in Russian | MEDLINE | ID: mdl-27141588

ABSTRACT

The neural apparatus and the endocrine part of the pancreas was studied in Wistar rats aged 3-4 and 19 months (n = 24) using the immunohistochemical reactions for synaptophysin (Syn), tyrosine hydroxylase (TH) and protein gene product 9.5 (PGP 9.5). Since Syn and PGP 9.5 are highly selective in detection of pancreatic islet (PI) endocrinocytes, it was possible to examine their topography and density in all parts of the pancreas. It was found that in rats aged 19 months, the total number of PI was decreased as compared to that in young animals. The study of PI size distribution has shown that the number of large islets decreased with age. Young animals showed rich innervation of the pancreas which was represented by three nerve plexuses: the first was a broadly-looped one, formed by small nerve trunks and bundles of unmyelinated and myelinated nerve fibers, the second consisted of thin bundles of postganglionic axons and microganglia, and the third (main terminal plexus) was formed by axons with varicosities and synapses of "en passant" type. In aged rats, marked degenerative changes in the neurons of intramural ganglia, nerve trunks and bundles were noted together with the reduction or complete absence of Syn- and TH-positive efferent parasympathetic and sympathetic terminals around blood vessels, excretory ducts, denervation of the exocrine and endocrine parts of the pancreas. Innervation disturbances in some lobules were accompanied by small inflammatory perivascular infiltrates.


Subject(s)
Aging/pathology , Islets of Langerhans/innervation , Islets of Langerhans/pathology , Aging/metabolism , Animals , Ganglia, Autonomic/metabolism , Ganglia, Autonomic/ultrastructure , Immunohistochemistry , Islets of Langerhans/metabolism , Nerve Fibers/metabolism , Nerve Fibers/ultrastructure , Presynaptic Terminals/metabolism , Presynaptic Terminals/ultrastructure , Rats, Wistar , Synaptophysin/metabolism , Tyrosine 3-Monooxygenase/metabolism
3.
Morfologiia ; 143(3): 77-80, 2013.
Article in Russian | MEDLINE | ID: mdl-24020190

ABSTRACT

In this paper we describe the modern immunohistochemical method detecting the neuronal marker PGP 9.5. The application of this method for the study of the innervation of rat and human heart, the detailed examination of the topography of the cardiac nervous apparatus, as well as their changes in pathological states, is demonstrated. Structural organization and the nature of the terminal branches of nerve apparatus suggest that they have afferent function. Protocol of the reaction demonstrating PGP 9.5 on paraffin sections is presented.


Subject(s)
Heart/innervation , Immunohistochemistry/methods , Myocardium/chemistry , Ubiquitin Thiolesterase/analysis , Animals , Humans , Nerve Fibers/chemistry , Rats
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