Detection of orange juice adulteration with beet medium invert sugar using anion-exchange liquid chromatography with pulsed amperometric detection.

Carbohydrate analysis of 5 beet medium invert sugar (BMIS) samples and 10 pure orange juices was carried out using anion-exchange chromatography with a pulsed amperometric detector. This analysis revealed the presence of several oligosaccharides in BMIS that were in either low concentration or nonexistent in the orange juice samples. These oligosaccharides may be naturally present in sugar beets or synthesized during the acid and/or enzyme catalyzed hydrolysis of sucrose during the production of BMIS. BMIS was intentionally added to pure orange juice at levels of 5, 10, 15, and 20%. Subsequent liquid chromatographic (LC) analysis of these intentionally adulterated samples revealed that detection of 5% BMIS in orange juice was possible.