Specimen Collection for Translational Studies in Hidradenitis Suppurativa.

Hidradenitis suppurativa (HS) is a chronic inflammatory disorder characterized by painful nodules, sinus tracts, and scars occurring predominantly in intertriginous regions. The prevalence of HS is currently 0.053-4%, with a predominance in African-American women and has been linked to low socioeconomic status. The majority of the reported literature is  retrospective, population based, epidemiologic studies. In this regard, there is a need to establish a repository of biospecimens, which represent appropriate gender and racial demographics amongst HS patients. These efforts will diminish knowledge gaps in understanding the disease pathophysiology. Hence, we sought to outline a step-by-step protocol detailing how we established our HS biobank to facilitate the formation of other HS tissue banks. Equipping researchers with carefully detailed processes for collection of HS specimens would accelerate the accumulation of well-organized human biological material. Over time, the scientific community will have access to a broad range of HS tissue biospecimens, ultimately leading to more rigorous basic and translational research. Moreover, an improved understanding of the pathophysiology is necessary for the discovery of novel therapies for this debilitating disease. We aim to provide high impact translational research methodology for cutaneous biology research and foster multidisciplinary collaboration and advancement of our understanding of cutaneous diseases.

[Clinical variants and symptomatic therapy of the climacteric syndrome]. / Klinicheskie varianty i simptomaticheskoe lechenie klimaktericheskogo sindroma.

The paper is concerned with the results of therapy of the climacteric syndrome: the 1st group--typical CS with predominant emotional-vegetative dystonia of vagoinsular nature, skin sensitivity to a certain fraction of estrogens was increased, the 2nd group--CS complicated by hypertension, skin reaction to progesterone; the 3rd group--CS with emotional-vegetative dystonia of mixed type, skin sensitivity to estrogens and progesterone. Intracutaneous administrations of fractions of estrogens (E) and progesterone (P) were at a ratio of E:P = 4:1 for the 1st group, E:P = 1:1 for the 2nd and 3rd groups, causing a complete convalescence of 77 of 94 patients and a partial convalescence of 17 patients. In case of a partial therapeutic effect diet- and balneotherapy was recommended.

[Plasma prolactin levels in men of various age groups]. / Soderzhanie prolaktina v plazme krovi muzhchin razlichnykh vozrastnykh grupp.

The blood plasma prolactin concentration was studied by radioimmunoassay in males of different age. The hormone level changed depending on age. A decrease in the reproductive function of the males over 40 years can be due to a fall of the blood plasma prolactin content.

[Tetragastrin activation of rat gastric mucosa adenyl cyclase in vitro]. / Aktivatsiia tetragastrinom adenilattsiklazy slizistoi obolochki zheludka krys in vitro.

The effect of tetragastrin and histamine on the adenylate cyclase activity of the rat gastric mucosa was examined in vitro. Tetragastrin and histamine were demonstrated to stimulate the activity of the enzyme in question. The antagonist of histamine H2-receptors, cimetidine inhibits the stimulatory effect of histamine in vitro and activates tetragastrin stimulation of the adenylate cyclase activity. It is suggested that tetragastrin and histamine activate adenylate cyclase of the rat gastric mucosa via different receptors.

[Importance of cooling after death for restoration of metabolism during resuscitation]. / Znachenie postmortal'nogo okhlazhdeniia dlia vosstanovleniia metabolizma pri ozhivleniia.

Incorporation of labelled metabolites into proteins, RNA and DNA was completely inhibited in rabbit tissues under conditions of prolonged hypothermia (10 degrees). The incorporation of labelled metabolites into all the biopolymers studied was restored after subsequent warming up to 38 degrees. Within 60-90 min after death of the animals due to acute anoxia, the labelled metabolites did not incorporate into protein, RNA and DNA. Artificial postmortal hypothermia (20 degrees) increased (by about 2.5-3-fold) the period of viability, during which reanimation is possible. The hypothermia enables subsequent restoration of the anabolic processes from the zero level.

Protein degradation to low-molecular compounds after death and during reanimation.

The process of protein degradation to amino acids and peptides in rabbits following death and during reanimation in terms of the effects of artificial postmortem cooling on that process has been studied. Protein degradation was judged by increase of low-molecular nitrogenous compounds in serum and in organs by increase in soluble radioactivity with time in animals the proteins of which had been marked in vivo with radioisotopes. It has been found that immediately after death resulting from acute anoxia the processes of protein degradation to amino acids as well as synthesis stops in liver, skeletal and cardiac muscles, spleen, brain and spinal cord. Similar phenomenon takes place in the case of deep hypothermy. During reanimation the process of protein degradation to low-molecular compounds in organs restores.

Restoration of nucleic acid biosynthesis after clinical death and factors stimulating the process in vivo.

The biosynthesis of RNA and DNA falls almost to zero in 60 min after the death of rabbits from anoxia, in all the organs of the body. Rapid artificial cooling of the rabbits to 20 degrees C undertaken within 10 min after death preserved nucleic acid biosynthesis and permitted restoration of life 3-4 h after death, with recovery beginning in 60 min. During the reanimation the addition of ATP to the blood stimulated the restoration of RNA biosynthesis in the spinal cord to a considerable extent; the addition of cocarboxylase to the blood promoted cardiac RNA biosynthesis as well as cardiac and pancreatic DNA biosynthesis during recovery.