ABSTRACT
A novel promising strain of actinobacteria Rhodococcus sp. 77-32 was identified. Its acetonetreated biomass the could be used as a biocatalyst for production of S-(-)-2-[6-benzyloxy-2,5,7,8-tetramethylchroman-2-yl] ethanol (S-BCE), a precursor of natural α-tocols. It was established that a reaction of enantioselective hydrolysis of racemic (±)-2-(2-acetoxyethyl)-6-benzyloxy-2,5,7,8-tetramethylchroman (BCEA) occurred in the phosphate bufferacetone system, resulting in enrichment of the residual substrate by S-enantiomer (S-(+)-2-(2-acetoxyethyl)-6-benzyloxy-2,5,7,8-tetramethylchroman, S-BCEA). It was shown that the hydrolysis was accompanied by stereoinversion of the formed product, R-(+)-2-[6-benzyloxy-2,5,7,8-tetramethylchroman-2-yl] ethanol (R-BCE), into the S-BCE. The transformation conditions (acetone content, acidity, temperature, reaction duration) were optimized, providing simultaneous production of optically pure S-BCE and S-BCEA with an almost quantitative yield.
Subject(s)
Chromans/chemical synthesis , Rhodococcus/chemistry , Tocopherols/chemical synthesis , Acetone/chemistry , Biocatalysis , Biomass , Chromans/isolation & purification , Hydrogen-Ion Concentration , Hydrolysis , Solvents/chemistry , Stereoisomerism , Temperature , Tocopherols/isolation & purificationABSTRACT
A procedure has been developed for the use of semiconductor laser radiation in the combined drug therapy of patients with disseminated infiltrative pulmonary tuberculosis with multiple destructions and massive bacterial excretion, by increasing the number of sessions, which reduces the time of bacterial excretion cessation by 2-4 weeks, decay cavity closure by a months, and hospital treatment by 1.5 months and promotes minimal residual changes in most of them.
Subject(s)
Laser Therapy , Tuberculosis, Pulmonary/radiotherapy , Adult , Aged , Antitubercular Agents/therapeutic use , Follow-Up Studies , Humans , Middle Aged , Radiotherapy, Adjuvant , Treatment Outcome , Tuberculosis, Pulmonary/drug therapyABSTRACT
The increase of DNA-synthesis rate (according incorporation [8-14C]adenine) in B12-deficient cells Propionibacterium shermanii as a result of different cobalamines adding into the cell suspension including metoxyethyladenile analog of adenozilcobalamin and some components of vitamin B12 molecule has been found. The DNA-synthesis rate in B12-deficient cells is nearly twice lower as compared with one in B12-normal cells. Considerable stimulative effect (80-100%) was provided with coenzyme forms of cobalamin. The data confirm the participation of vitamin B12 in DNA-synthesis in Propionibacterium cells.
