Functionalization and Modification of Polyethylene Terephthalate Polymer by AgCl Nanoparticles under Ultrasound Irradiation as Bactericidal.

Polyethylene terephthalate polymer (PET) is widely used in diverse areas. In the current study, the surface of PET is modified in two steps in order to improve the quality. At first, the polymer was functionalized with carboxylic groups, and Fourier transform infrared spectroscopy studies were used to verify functionalization. Then, AgCl nanoparticles were synthesized on COOH functional groups on the surface of PET using a sonochemistry method by sequential dipping of the functionalized polymer in an alternating bath of potassium chloride and silver nitrate under ultrasonic irradiation. The effects of ultrasonic irradiation power, the number of dipping steps, and pH on the growth of AgCl nanoparticles as effective parameters on size and density of synthesized Ag nanoparticles were studied. The results of scanning electron microscopy studies showed that the size and density of AgCl nanoparticles under ultrasonic irradiation with a power of 100 W are better than those of AgCl nanoparticles under irradiation with a power of 30 W. Also, by 15 times dipping the polymer into the reagent solutions in pH = 9, the modified polymer with a greater number of nanoparticles with suitable size can be reached. Antibacterial properties of PET containing AgCl nanoparticles were investigated against six Gram-positive and Gram-negative bacteria species, and the results showed significant antibacterial activity, while functionalized PET did not have a significant effect on both types of bacteria.

Ultra-sensitive electrochemical aptasensor for label-free detection of Aflatoxin B1 in wheat flour sample using factorial design experiments.

Considering the significance of mycotoxin detection in food industries, herein, an ultrasensitive aptasensor was developed based on aflatoxin B1 aptamer immobilized on Carbon quantum dots/octahedral Cu2O nanocomposite. Electrochemical measurements were based on Electrochemical Impedance Spectroscopy (EIS) and Differential Pulse Voltammetry (DPV). Since the effective parameters (pH, temperature, incubation time and concentration of aptamers) are interdependent, so their dependent study can be nonideal. Taguchi method has solved this problem and optimized the experimental conditions using a smaller number of experiments. Under optimum conditions, the electrochemical signals declined as AFB1 concentrations increased with a dynamic range of 3 ag.ml-1 -1.9 µg.ml-1 and a low limit of detection (LOD) of 0.9 ± 0.04 ag ml-1. The obtained results proved sufficient repeatability (RSD = 2.4%), reproducibility (RSD = 2.56%), accuracy (97.2-104.4% recovery), and robustness (RSD = 3.25%). Furthermore, considerable selectivity, stability and reliability of the aptasensor confirmed the capability to work in future real assays.

Blue luminescent graphene quantum dot conjugated cysteamine functionalized-gold nanoparticles (GQD-AuNPs) for sensing hazardous dye Erythrosine B.

Erythrosine B (ErB) is a xanthenes approved dye that widely used in various fields such as foods, drugs and cosmetics. This work intended to analyze the interaction of this dye on synthesized graphene quantum dot conjugated cysteamine functionalized gold nanoparticles. Pyrolysis of citric acid was applied to graphene quantum dots (GQDs) synthesis. These are zero dimensional materials promising considerable applications because of their extraordinary physicochemical properties. In this work, to improve its applications conjugates of GQDs and gold nanoparticles (GQD-AuNPs) are synthesized and characterized with FESEM, TEM, EDX and FTIR techniques. Interaction of ErB with synthesized conjugates was investigated using fluorescence technique and the results demonstrate that ErB can quench fluorescence of GQD-AuNPs conjugates, considerably. At last, we used of this good interaction for construction sensor for detection of ErB in the concentration range of 1.2 nM to 50 nM and the obtained LOD was 0.03 nM (S/N = 3) with correlation coefficient of (99%).

Experimental and Computational Evidence on the Interaction of Cycloalkyl α-Aminobisphosphonates with Calf Thymus DNA.

Fluorescence spectroscopy, ultraviolet-visible absorption spectroscopy, circular dichroism spectroscopy, viscometry, cyclic voltammetry, and differential pulse voltammetry were applied to investigate the competitive interaction of DNA with the three new cycloalkyl α-aminobisphosphonates (D1-D3) and spectroscopic probe, neutral red dye, and Hoechst (HO), in a Tris-hydrogen chloride buffer (pH 7.4). The spectroscopic and voltammetric studies showed that the groove binding mode of interaction is predominant in the solution containing DNA and α-aminobisphosphonates. Furthermore, the results indicated that α-aminobisphosphonate with the lengthy N alkyl chains and larger heterocyclic ring size had a stronger interaction. The principal component analysis and theoretical quantum mechanical and molecular mechanics (QM-DFT B3LYP/6-31+G* and MM-SYBYL) methods were also applied to determine the number of chemical components presented in complexation equilibrium and identify the structure complexes of DNA with the three new cycloalkyl α-aminobisphosphonates (D1-D3), respectively.

Assessment of the living and workplace health and safety conditions of site-resident construction workers in Tehran, Iran.

OBJECTIVES: The purpose of this study was to assess living and workplace safety conditions of construction workers in Tehran, Iran. METHODS: This cross-sectional study was conducted among 410 construction sites in a municipal area of Tehran whose municipal building permits were issued in 2011. Data on ventilation, workplace safety and hygiene were collected by direct observation and interviews with site foremen. Noise levels were estimated from 10 sound-level-meter stations in the municipality area. RESULTS: Lack of ventilation in the workers' rooms was abundant. Bathrooms were unhygienic and minimum requirements such as lighting and ventilation did not exist in 80% of the cases. In nearly 50% of large construction sites, sewage and garbage disposal were inappropriate. Elevator safety was poor at all sites and no measures for fall prevention were present in over 88% of active construction sites. This study showed that the mean 24-h equivalent continuous sound level Leq was over 70 dB in 80% of the sites during weekdays. CONCLUSIONS: The results of this study revealed poor health and safety living and working conditions of construction workers in Tehran.

DNA interaction of [Cu(dmp)(phen-dion)] (dmp=4,7 and 2,9 dimethyl phenanthroline, phen-dion=1,10-phenanthroline-5,6-dion) complexes and DNA-based electrochemical biosensor using chitosan-carbon nanotubes composite film.

The interaction of two new water-soluble [Cu(4,7-dmp)(phen-dione)Cl]Cl (1) and [Cu(2,9-dmp)(phen-dione)Cl]Cl (2) which dmp is dimethyl-1,10-phenanthroline and phen-dion represents 1,10-phenanthroline-5,6-dion, with DNA in solution and immobilized DNA on a chitosan-carbon nanotubes composite modified glassy carbon electrode were investigated by cyclic voltammetry and UV-Vis spectroscopy techniques. In solution interactions, spectroscopic and electrochemical evidences indicate outside binding of these complexes. To clarify the binding mode of complexes, it was done competition studies with Hoechst and Neutral red as groove binder and intercalative probes, respectively. All these results indicating that, these two complexes (1) and (2) interact with DNA via groove binding and partially intercalative modes, respectively. The electrochemical characterization experiments showed that the nanocomposite film of chitosan-carbon nanotubes could effectively immobilize DNA and greatly improve the electron-transfer reactions of the electroactive molecules that latter finding is the result of strong interactions between captured DNA and Cu complexes. This result indicates that these complexes could be noble candidates as hybridization indicators in further studies. At the end, these new complexes showed excellent antitumor activity against K562 (human chronic myeloid leukemia) cell lines.