Subject(s)
Antigens, Neoplasm , Antigens, Surface , Lactate dehydrogenase-elevating virus/pathogenicity , Lymphoma, Non-Hodgkin/immunology , Neoplasm Proteins , Animals , Lymphoma, Non-Hodgkin/microbiology , Male , Mice , Mice, Inbred C3H , Neoplasm Transplantation , Sarcoma, Experimental/immunology , Sarcoma, Experimental/microbiology , Tissue ExtractsSubject(s)
Abortion, Spontaneous/immunology , Genital Neoplasms, Female/immunology , Immunologic Techniques , Lactate dehydrogenase-elevating virus/immunology , Leukocyte Adherence Inhibition Test , Animals , Antigens, Viral/analysis , Female , Humans , Male , Mice , Myocardial Infarction/immunology , Neoplasms/immunology , PregnancyABSTRACT
Immunochemically active fractions were obtained using Separon Hema-300-glc(R) from serum of lactic dehydrogenase virus (LDV) infected mice and from homogenates of human tumors. The mixture of proteins of tumorous origin from the cytosol giving a positive reaction in the leukocyte adherence inhibition (LAI) test was found in the same fractions showing maximum of absorbance at 340 nm in the spectrophotometer and a corresponding peak in the refractometer. Analogous peaks were not proved in material obtained from healthy controls, but they were found in some human placentas and fetal organs. The LDV fraction obtained from mouse serum served as a "control" antigen in LAI test for human tumor testing, and results corresponded with those obtained using cytosol specific for the tumor under study.
Subject(s)
Cytosol/immunology , Immune Sera/immunology , Immunologic Techniques , Lactate dehydrogenase-elevating virus/immunology , Leukocyte Adherence Inhibition Test , Neoplasms/immunology , Virus Diseases/immunology , Animals , Chromatography, Gel , Female , Humans , L-Lactate Dehydrogenase/blood , Mice , Mice, Inbred AKR , Mice, Inbred C3H , Neoplasms, Experimental/immunologyABSTRACT
A method for the rapid separation of proteinous fractions by high-performance gel chromatography was described. Homogenates from tumorous and healthy tissues and blood were eluted by saline on a column packed with rigid hydrophilic macroporous particles of O-glucose--Spheron 300. Fractions were collected and subjected to further analyses. Their antigenic activity was determined by the leucocyte adherence inhibition test method. For the specific immunoactive fractions a dependence of leucocyte adherence inhibition test values on the clinical state of sample donators has been found.
Subject(s)
Neoplasm Proteins/analysis , Animals , Chromatography, Gel/methods , Female , Humans , Immunochemistry , Leukocyte Adherence Inhibition Test , Male , Mice , Neoplasm Proteins/blood , Neoplasms, Experimental/analysis , Neoplasms, Experimental/bloodABSTRACT
In conclusion we can claim that the determination of total LDH activity in the amniotic fluid and the mother's blood serum is not a suitable method for diagnosing intrauterine danger to the foetus from hypoxia. It is interesting to note that the high activity of the third and fourth LDH fraction in the amniotic fluid does not correspond to their activity in the mother's serum.
Subject(s)
Amniotic Fluid/enzymology , Clinical Enzyme Tests , Fetal Hypoxia/diagnosis , L-Lactate Dehydrogenase/metabolism , Pregnancy Complications/diagnosis , Female , Humans , Infant, Newborn , Isoenzymes , L-Lactate Dehydrogenase/blood , PregnancySubject(s)
Clinical Enzyme Tests , Infant, Premature , L-Lactate Dehydrogenase/blood , Fetus , Gestational Age , Humans , Infant, Newborn , IsoenzymesABSTRACT
In the First Gynaecology and Maternity Clinic of the Faculty of Medicine, Charles University Prague, we investigated total serum LDH (L-lactate: NAD-oxidoreductase, E.C.1.1.1.27) activity and that of its 5 isoenzymes during physiological pregnancy. We divided our pregnancies, for practical purposes, into 3 groups. The first comprised women in the 10th-to 20th week of pregnancy, the second women in the 20th to 30th week and the third women in the 30th to 40th week. In each group we took blood dynamically at regular 14-day intervals from the same women, with the aim of assuring the maximum possible homogeneity of the tested material (blood) in correlation to time. We found that, during physiological pregnancy, neither the total LDH activity level, nor that of its five fractions, exceeded the norm determined for the Czechoslovak population. The activities fluctuated typically during pregnancy, however, within the framework of the norm. Some agreement was found between changes in LDH activity and changes in the chorionic gonadotrophin level.
Subject(s)
L-Lactate Dehydrogenase/blood , Pregnancy , Female , Humans , Isoenzymes , Pregnancy Trimester, First , Pregnancy Trimester, Second , Pregnancy Trimester, ThirdABSTRACT
We investigated the serum activity of the fifth fraction of L-lactate: NAD-oxidoreductase (E.C.1.1.1.27) and serum alkaline phosphatase activity prior to diagnostic curettage in patients with abnormal and atypical endomertial hyperplasia. We also determined them in 75 patients with carcinoma of the endometrium, 80 patients with glandular cystic endometrial hyperplasia and a "normal" control group of 70 patients. In the statistical evaluation we found a significantly high incidence of low AP activity and elevated LDH5 in the group of patients with glandular cystic endometrial hyperplasia, as well as in patients with precancerous lesions of the endometrium and carcinoma of the endometrium. The findings raise the question of whether these changes in the serum activity of the given enzymes are only a response to a given hormonal modulation.
Subject(s)
Alkaline Phosphatase/blood , Clinical Enzyme Tests , L-Lactate Dehydrogenase/blood , Precancerous Conditions/diagnosis , Uterine Neoplasms/diagnosis , Endometrium/pathology , Female , Humans , Hyperplasia/diagnosis , Isoenzymes , Uterine Neoplasms/pathologyABSTRACT
Gel chromatography on Spheron P-500 of mouse serum yielded fractions elevating lactate dehydrogenase activity. The order of elution was viral activity, proteins and lactate dehydrogenase activity. Viral contamination occurred also in certain fractions subjected to repeated gel chromatography in which no proteins were detectable.
