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1.
Clin Chem ; 36(1): 164-7, 1990 Jan.
Article in English | MEDLINE | ID: mdl-2297919

ABSTRACT

Very high concentrations of CA 125 have been found in some ovarian cancer patients after repeated radioimmunodetection with anti-CA 125 antibodies [OC125-F(ab')2]. In one patient we measured a CA 125 concentration of 135,000 kilo-arb. units/L, using an enzyme immunoassay involving OC125 antibodies. With an immunoradiometric assay involving use of two new anti-CA 125 antibodies (B43.13 and B27.1), the CA 125 concentration was 34 kilo-arb. units/L, indicating a discrepancy. The component responsible for the high result in the enzyme immunoassay could be purified by immunoaffinity chromatography on Protein A-Sepharose. Furthermore this component bound to anti-human IgG-Sepharose in the same manner as did the serum IgG fraction. Adsorption of human anti-mouse antibodies present in the serum did not decrease the CA-125-like material. Binding of whole OC125 antibodies to the purified CA-125-like material was inhibited completely in the presence of CA 125 antigen. We infer that the false-positive CA 125 activity is ascribable to a human IgG directed against an idiotope of the OC125 antibody, which was induced by repeated application of OC125 antibodies. To avoid falsely positive results in patients receiving OC125 antibodies, CA 125 should be measured by an assay in which other antibodies are used.


Subject(s)
Antibodies, Anti-Idiotypic/analysis , Antigens, Tumor-Associated, Carbohydrate/analysis , Aged , Antibodies/immunology , Antigens, Tumor-Associated, Carbohydrate/immunology , Binding Sites, Antibody , Chromatography, Affinity , Cross Reactions , False Positive Reactions , Female , Humans , Immunochemistry , Immunoglobulin G/analysis , Ovarian Neoplasms/diagnostic imaging , Ovarian Neoplasms/immunology , Radionuclide Imaging , Reagent Kits, Diagnostic
2.
Physiol Behav ; 45(6): 1101-5, 1989 Jun.
Article in English | MEDLINE | ID: mdl-2813534

ABSTRACT

Experiments were performed to determine if the inhibition of copulatory behavior observed in male rats with chronically elevated serum prolactin levels (hyperprolactinemia) is associated with changes in central dopaminergic function in the nigrostriatal and mesolimbic systems. Chronic hyperprolactinemia, induced by ectopic pituitary grafts, inhibited sexual activity but was not associated with changes in locomotor activity, serotyped behavior in response to various doses of apomorphine, or 3H-spiroperidol binding to striatal homogenates. However, open-field defecation was reduced in the pituitary grafted animals. The results of the present study show that changes in nigrostriatal dopamine receptor sensitivity do not contribute to the inhibition of sexual behavior in hyperprolactinemic male rats. In addition, these results also demonstrate that the effects of hyperprolactinemia are relatively specific to copulatory behavior and appear not to involve general behavioral suppression.


Subject(s)
Brain/metabolism , Dopamine/physiology , Hyperprolactinemia/physiopathology , Sexual Behavior, Animal/physiology , Stereotyped Behavior/physiology , Animals , Apomorphine/pharmacology , Copulation/physiology , Dose-Response Relationship, Drug , Male , Motor Activity/physiology , Rats , Rats, Inbred F344 , Receptors, Dopamine/metabolism , Spiperone/metabolism , Stereotyped Behavior/drug effects
3.
Neuropharmacology ; 23(7A): 773-7, 1984 Jul.
Article in English | MEDLINE | ID: mdl-6089023

ABSTRACT

The intravenous administration of pentobarbital, phenobarbital or barbital produced a dose-related depression of the levels of cyclic guanosine monophosphate (cGMP) in the cerebellum of male Sprague-Dawley rats. This depression of cGMP occurred with doses of pentobarbital and barbital which did not reduce locomotor activity. Further, the time-course of the recovery of locomotor activity following the administration of an anesthetic dose of pentobarbital preceded that for the recovery of the levels cGMP in the cerebellum. In a separate study, pretreatment with picrotoxinin reversed the significant depression of cGMP in the cerebellum produced by the smallest dose of pentobarbital. Collectively, these data suggest that the effects of the barbiturates on cGMP in the cerebellum are not exclusively or predominantly the result of a drug-related depression of locomotor activity and may be, at least partially, mediated via a barbiturate-induced potentiation of a GABA receptor-mediated mechanism.


Subject(s)
Barbiturates/pharmacology , Cerebellum/drug effects , Cyclic AMP/metabolism , Animals , Barbital/pharmacology , Cerebellum/metabolism , Dose-Response Relationship, Drug , Male , Motor Activity/drug effects , Pentobarbital/pharmacology , Phenobarbital/pharmacology , Picrotoxin/pharmacology , Rats , Rats, Inbred Strains , Receptors, Cell Surface/drug effects , Receptors, GABA-A
6.
Brain Res ; 173(1): 47-56, 1979 Sep 07.
Article in English | MEDLINE | ID: mdl-487084

ABSTRACT

Adult male Sprague-Dawley rats were given hourly injections of physostigmine for 1--4 h, and the effect of this treatment on dopamine (DA) and noradrenaline (NA) content or on DA and NA was estimated by measuring the decline in these amines produced following the inhibition of tyrosine hydroxylase with alpha-methyl-p-tyrosine (alpha MPT). In later experiments oxotremorine was administered instead of physostigmine at hourly intervals for 2 h. Physostigmine administration resulted in a highly significant increase in the depletion of NA produced by alpha MPT indicating that the turnover of NA was increased by this drug. This effect was observed in the medial basal hypothalamus and anterior hypothalamus but not in the telencephalon-thalamus. Oxotremorine also produced an increase in NA turnover, but this drug was effective in all three brain areas. Atropine pretreatment blocked the effect of both physostigmine and oxotremorine on NA turnover. However, in the case of physostigmine, atropine was only effective if it was given 30 min before each injection of physostigmine. Mecamylamine, a nicotine blocker, did not reverse the effect of physostigmine on NA turnover. These results suggest that there is a cholinergic input via muscarinic receptors which influences the activity of noradrenergic pathways terminating in the anterior or medial basal hypothalamus.


Subject(s)
Dopamine/metabolism , Hypothalamus, Anterior/drug effects , Hypothalamus, Middle/drug effects , Hypothalamus/drug effects , Norepinephrine/metabolism , Parasympathomimetics/pharmacology , Animals , Atropine/pharmacology , Luteinizing Hormone/blood , Male , Methyltyrosines/pharmacology , Neural Pathways/drug effects , Oxotremorine/pharmacology , Physostigmine/pharmacology , Prolactin/blood , Rats , Telencephalon/drug effects , Thalamus/drug effects
11.
Brain Res ; 117(1): 77-84, 1976 Nov 19.
Article in English | MEDLINE | ID: mdl-1033022

ABSTRACT

Groups of male golden hamsters and Sprague-Dawley rats were sacrificed at 4-h intervals over a 24-h period. Tryptophan and serotonin (5-HT) were measured in the telencephalon and brain stem and tryptophan was measured in serum. Highly statistically significant changes in tryptophan and 5-HT content were observed over the period of the day. Generally the changes were more clearly defined and were significant to a higher statistical level in the hamster than in the rat. Statistically significant changes were also observed in serum tryptophan. In the hamster the daily changes in serum tryptophan were coincidental with similar changes in tryptophan and 5-HT in the brain stem and tryptophan in the telencephalon. On the other hand, the changes in 5-HT in the hamster telencephalon were not well correlated with changes in tryptophan and serum tryptophan.


Subject(s)
Brain/metabolism , Circadian Rhythm , Serotonin/metabolism , Tryptophan/metabolism , Animals , Brain Stem/metabolism , Cricetinae , Male , Rats , Telencephalon/metabolism , Tryptophan/blood
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