A hybrid catalyst-triggered cascade reaction for cholesterol detection using a smartphone-based miniature fluorescent apparatus.

A new hybrid biological-chemical catalyst, magnetic nanoparticles functionalized with cholesterol oxidase (Fe3O4/APTES/ChOx), was developed for cholesterol detection. In the presence of cholesterol, the enzyme produced H2O2, which facilitated the generation of fluorescent molecules from the fluorogenic substrate with the assistance of Fe3O4 nanoparticles. A smartphone camera with a miniature fluorescent apparatus was used to assess fluorescence emission. Then, a smartphone application was employed to translate the fluorescence intensity to the red, green, and blue (RGB) domain. The developed approach achieved excellent selectivity and acceptable performances while supporting an onsite analysis approach. The practical operational range spanned from 5 to 100 nM, with a detection limit of 0.85 nM. Fe3O4/APTES/ChOx was applied for up to four replicates of reuse and demonstrated stability for at least 30 days. The applicability of the method was evaluated in milk samples, and the results were in accordance with the reference method.

A portable fluorescence detection device based on a smartphone employing carbon nanodots for Mn2+ sensing.

The measurement of fluorescence emission for quantitative analysis is typically based on a traditional spectrofluorometer, which limits an onsite detection approach. Thus, an alternative device should be developed for fulfilling this analysis outside of the laboratory. Therefore, a low-cost, portable, and low-energy consumption fluorescence reader-based smartphone device was developed. An ultraviolet light-emitting diodes (UV-LED) was used to construct the fluorescence device-based smartphone as a low-power excitation light source. The smartphone camera was used as a detector for detecting photons from the fluorescence emission process of the fluorescence probe and was connected to a digital image platform. Transparent acrylic with orange and yellow colors was employed as a filter for reducing the interference from light source intensity. The obtained digital image was converted to red, green and blue (RGB) intensity using a custom-designed smartphone application. N,S-doped carbon nanodots (N,S-CDs) were demonstrated to be a good fluorescence indicator for determining trace quantities of Mn2+ in cosmetics. The approach exhibited high selectivity and sensitivity, detecting and quantifying analytes at 1-5 µM concentrations. Furthermore, the method's detection limit of 0.5 µM reflects its capacity to detect trace amounts of a target analyte. Mn2+ in cosmetic products was successfully analyzed using this device with high accuracy comparable with the results from inductively coupled plasma-optical emission spectroscopy (ICP-OES).

Smartphone-based portable fluorescence sensor with gold nanoparticle mediation for selective detection of nitrite ions.

A simple, portable device for the detection of NO2- via a fluorescence method was developed. The proposed device consisted of a dark box containing a blue LED as a low-power excitation light source and a smartphone with a mobile application for RGB analysis as a light detector. Detection was mediated by using synthesized cetyltrimethylammonium bromide-stabilized gold nanoparticles (CTAB-AuNPs). The CTAB-AuNPs were etched with NO2- to yield Au3+, which catalyzes the oxidation of o-phenylenediamine (OPD) in the presence of H2O2 to generate 2,3-diaminophenazine (DAP). Triton X-100 (TX-100) micelles were introduced to improve the DAP fluorescence emission. The fluorescence intensity of DAP was recorded by the smartphone in terms of RGB intensity, which was correlated with the NO2- concentration. This method provided a wide linear working concentration range (0.5-100 µM), a limit of detection of 0.17 µM and excellent selectivity for NO2- over other anions.