Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 12 de 12
Filter
Add more filters










Publication year range
1.
Dev Biol ; 334(2): 383-94, 2009 Oct 15.
Article in English | MEDLINE | ID: mdl-19665013

ABSTRACT

Sea urchins provide an excellent model for studying cell cycle control mechanisms governing DNA replication in vivo. Fertilization and cell cycle progression are tightly coordinated by Ca(2+) signals, but the mechanisms underlying the onset of DNA replication after fertilization remain less clear. In this study we demonstrate that calcium-dependent activation of ERK1 promotes accumulation of cyclinE/cdk2 into the male and female pronucleus and entry into first S-phase. We show that cdk2 activity rises quickly after fertilization to a maximum at 4 min, corresponding in timing to the early ERK1 activity peak. Abolishing MAP kinase activity after fertilization with MEK inhibitor, U0126, substantially reduces the early peak of cdk2 activity and prevents cyclinE and cdk2 accumulation in both sperm pronucleus and zygote nucleus in vivo. Both p27(kip1) and roscovitine, cdk2 inhibitors, prevented DNA replication suggesting cdk2 involvement in this process in sea urchin. Inhibition of cdk2 activity using p27(kip1) had no effect on the phosphorylation of MBP by ERK, but completely abolished phosphorylation of retinoblastoma protein, a cdk2 substrate, indicating that cdk2 activity is downstream of ERK1 activation. This pattern of regulation of DNA synthesis conforms to the pattern observed in mammalian somatic cells.


Subject(s)
Cyclin E/physiology , Cyclin-Dependent Kinase 2/physiology , DNA Replication , Lytechinus/embryology , MAP Kinase Signaling System , Animals , Cyclin-Dependent Kinase Inhibitor p27/physiology , Embryo, Nonmammalian/enzymology , Female , Male , Mice , Microinjections , Proliferating Cell Nuclear Antigen/physiology , Protein Transport , Recombinant Fusion Proteins/physiology , Retinoblastoma Protein/physiology , S Phase
2.
J Cell Sci ; 113 ( Pt 15): 2659-70, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10893181

ABSTRACT

The proteasome has been shown to be involved in exit from mitosis by bringing about destruction of mitotic cyclins. Here, we present evidence that the proteasome is also required for proper completion of S phase and for entry into mitosis in the sea urchin embryonic cleavage cycle. A series of structurally related peptide-aldehydes prevent nuclear envelope breakdown in their order of inhibitory efficacies against the proteasome. Their efficacies in blocking exit from S phase and exit from mitosis correlate well, indicating that the proteasome is involved at both these steps. Mitotic histone HI kinase activation and tyrosine dephosphorylation of p34(cdc2) kinase are blocked by inhibition of the proteasome, indicating that the proteasome plays an important role in the pathway that leads to embryonic p34(cdc2 )kinase activation. Arrested embryos continued to incorporate [(3)H]thymidine and characteristically developed large nuclei. Pre-mitotic arrest can be overcome by treatment with caffeine, a manoeuvre that is known to override the DNA replication checkpoint. These data demonstrate that the proteasome is involved in the control of termination of S phase and consequently in the initiation of M phase of the first embryonic cell cycle.


Subject(s)
Cysteine Endopeptidases/metabolism , DNA Replication/physiology , Mitosis/genetics , Multienzyme Complexes/metabolism , Sea Urchins/physiology , Animals , CDC2 Protein Kinase/genetics , CDC2 Protein Kinase/metabolism , Caffeine/pharmacology , Cell Division/drug effects , Cell Division/genetics , Cell Nucleus/physiology , DNA Replication/drug effects , Embryo, Nonmammalian/physiology , Emetine/pharmacology , Female , Gene Expression Regulation, Developmental/drug effects , Gene Expression Regulation, Developmental/physiology , Gene Expression Regulation, Enzymologic/drug effects , Gene Expression Regulation, Enzymologic/physiology , Leupeptins/pharmacology , Mitosis/drug effects , Nuclear Envelope/physiology , Phenotype , Phosphodiesterase Inhibitors/pharmacology , Phosphorylation , Phosphotyrosine/metabolism , Protease Inhibitors/pharmacology , Proteasome Endopeptidase Complex , Protein Kinases/genetics , Protein Kinases/metabolism , Protein Synthesis Inhibitors/pharmacology , S Phase/drug effects , S Phase/genetics , Thymidine/pharmacokinetics , Tritium
3.
J Biol Chem ; 274(12): 7958-68, 1999 Mar 19.
Article in English | MEDLINE | ID: mdl-10075693

ABSTRACT

The human tyrosine phosphatase (p54(cdc25-c)) is activated by phosphorylation at mitosis entry. The phosphorylated p54(cdc25-c) in turn activates the p34-cyclin B protein kinase and triggers mitosis. Although the active p34-cyclin B protein kinase can itself phosphorylate and activate p54(cdc25-c), we have investigated the possibility that other kinases may initially trigger the phosphorylation and activation of p54(cdc25-c). We have examined the effects of the calcium/calmodulin-dependent protein kinase (CaM kinase II) on p54(cdc25-c). Our in vitro experiments show that CaM kinase II can phosphorylate p54(cdc25-c) and increase its phosphatase activity by 2.5-3-fold. Treatment of a synchronous population of HeLa cells with KN-93 (a water-soluble inhibitor of CaM kinase II) or the microinjection of AC3-I (a specific peptide inhibitor of CaM kinase II) results in a cell cycle block in G2 phase. In the KN-93-arrested cells, p54(cdc25-c) is not phosphorylated, p34(cdc2) remains tyrosine phosphorylated, and there is no increase in histone H1 kinase activity. Our data suggest that a calcium-calmodulin-dependent step may be involved in the initial activation of p54(cdc25-c).


Subject(s)
Calcium-Calmodulin-Dependent Protein Kinases/metabolism , Cell Cycle Proteins/metabolism , G2 Phase , Mitosis , Peptides/metabolism , Phosphoprotein Phosphatases/metabolism , cdc25 Phosphatases , Benzylamines/pharmacology , CDC2 Protein Kinase/metabolism , Calcium-Calmodulin-Dependent Protein Kinase Type 2 , Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors , Enzyme Activation , Enzyme Inhibitors/pharmacology , HeLa Cells , Humans , Okadaic Acid/pharmacology , Phosphorylation , Protein Tyrosine Phosphatases/metabolism , Sulfonamides/pharmacology
4.
J Cell Sci ; 111 ( Pt 17): 2497-505, 1998 Sep.
Article in English | MEDLINE | ID: mdl-9701549

ABSTRACT

A MBP kinase activity increases at mitosis during the first two embryonic cell cycles of the sea urchin embryo. The activity profile of the MBP kinase is the same both in whole cell extracts and after immunoprecipitation with an anti-MAP kinase antibody (2199). An in-gel assay of MBP activity also shows the same activity profile. The activity is associated with the 44 kDa protein that cross-reacts with anti-MAP kinase antibodies. The 44 kDa protein shows cross-reactivity to anti-phosphotyrosine and MAP kinase-directed anti-phosphotyrosine/phosphothreonine antibodies at the times that MBP kinase activity is high. The 2199 antibody co-precipitates some histone H1 kinase activity, but the MBP kinase activity cannot be accounted for by histone H1 kinase-dependent phosphorylation of MBP. The MAP kinase 2199 antibody was used to purify the MBP kinase activity. Peptide sequencing after partial digestion shows the protein to be homologous to MAP kinases from other species. These data demonstrate that MAP kinase activation during nuclear division is not confined to meiosis, but also occurs during mitotic cell cycles. MAP kinase activity in immunoprecipitates also increases immediately after fertilization, which in the sea urchin egg occurs at interphase of the cell cycle. Treating unfertilized eggs with the calcium ionophore A23187 stimulates the increase in MAP kinase activity, demonstrating that a calcium signal can activate MAP kinase and suggesting that the activation of MAP kinase at fertilization is due to the fertilization-induced increase in cytoplasmic free calcium concentration. This signalling pathway must differ from the pathway responsible for calcium-induced inactivation of MAP kinase activity that is found in eggs that are fertilized in meiotic metaphase.


Subject(s)
Calcium-Calmodulin-Dependent Protein Kinases/metabolism , Embryo, Nonmammalian/cytology , Embryo, Nonmammalian/enzymology , Mitosis/physiology , Amino Acid Sequence , Animals , CDC2 Protein Kinase/metabolism , Calcium/metabolism , Calcium/physiology , Calcium-Calmodulin-Dependent Protein Kinases/chemistry , Calcium-Calmodulin-Dependent Protein Kinases/physiology , Cell Cycle/physiology , Enzyme Activation/drug effects , Fertilization/physiology , Glycogen Synthase Kinase 3 , Microtubule-Associated Proteins , Molecular Sequence Data , Molecular Weight , Peptides/chemistry , Peptides/isolation & purification , Sea Urchins , Sequence Analysis , Signal Transduction , Subcellular Fractions/enzymology
5.
Eur J Cell Biol ; 73(1): 58-70, 1997 May.
Article in English | MEDLINE | ID: mdl-9174672

ABSTRACT

A major nucleolar phosphoprotein, B23, is thought to play several apparently unrelated roles and appears to be associated with other cell compartments besides the nucleolus. However, characteristic properties of B23 variants still remain to be established. Here, we raised a new monoclonal antibody against B23 (20B2) and used it to address the issue particularly focusing on the events during mitosis. Also, we made an attempt to generalize the data on the cell cycle-dependent translocations of B23 by the use of three mammalian cell lines (HeLa, PK, RAMT) which were found to be immunoreactive for 20B2. In all the cell strains studied, B23 was chiefly located within the nucleolus at interphase, and was associated with a few cellular domains during mitosis. They were: the nucleoplasm (at prophase before the nuclear envelope breakdown), the cytoplasm (from prometaphase until mid telophase), the perichromosomal layer (from prometaphase till early telophase), cytoplasmic B23-containing bodies (at anaphase and telophase) and prenucleolar bodies, PNBs (at telophase). On Western blots, electrophoretic mobility of B23 was found to be the same at G1, S and G2 periods of interphase, but became slower at mitosis. In situ and cell extraction experiments showed that like the nucleolar B23, B23 of the perichromosomal layer and that of PNBs was highly resistant to extraction with Triton X-100, but could be released with Triton X-100/RNase A. These findings indicated that these portions of B23 were most likely to be associated with RNA. The cytoplasmic B23 was the major intracellular variant of B23 during mitosis. It had a slightly lower electrophoretic mobility than the perichromosomal B23 and could readily be extracted with Triton X-100 without addition of RNase A, a fact indicating that the cytoplasmic B23 was mainly in an RNA-free state. Mitosis-like translocations of B23 from the nucleolus to the nucleoplasm induced by actinomycin D increased its extractability but did not affect the electrophoretic mobility. The phosphorylation status of different B23 variants at interphase and mitosis both in controls and following the drug, is discussed.


Subject(s)
Cell Cycle , Cell Nucleolus/metabolism , Nuclear Proteins/metabolism , 3T3 Cells , Animals , Antibodies, Monoclonal , Biological Transport/drug effects , CHO Cells , Cattle , Cell Cycle/drug effects , Cell Cycle/immunology , Cell Line , Chlorocebus aethiops , Cricetinae , Dactinomycin/pharmacology , Deoxyribonuclease I/pharmacology , Dichlororibofuranosylbenzimidazole/pharmacology , HeLa Cells , Humans , Interphase/drug effects , Mice , Mitosis/drug effects , Nuclear Proteins/chemistry , Nuclear Proteins/drug effects , Nuclear Proteins/immunology , Nucleophosmin , Octoxynol/pharmacology , Rats , Ribonuclease, Pancreatic/pharmacology , Swine
6.
J Cell Sci ; 107 ( Pt 1): 253-65, 1994 Jan.
Article in English | MEDLINE | ID: mdl-8175912

ABSTRACT

Molecular cloning and characterisation of a human nuclear protein designated BM28 is reported. On the amino acid level this 892 amino acid protein, migrating on SDS-gels as a 125 kDa polypeptide, shares areas of significant similarity with a recently defined family of early S phase proteins. The members of this family, the Saccharomyces cerevisiae Mcm2p, Mcm3p, Cdc46p/Mcm5p, the Schizosaccharomyces pombe Cdc21p and the mouse protein P1 are considered to be involved in the onset of DNA replication. The highest similarity was found with Mcm2p (42% identity over the whole length and higher than 75% over a conservative region of 215 amino acid residues), suggesting that BM28 could represent the human homologue of the S. cerevisiae MCM2. Using antibodies raised against the recombinant BM28 the corresponding antigen was found to be localised in the nuclei of various mammalian cells. Microinjection of anti-BM28 antibody into synchronised mouse NIH3T3 or human HeLa cells presents evidence for the involvement of the protein in cell cycle progression. When injected in G1 phase the anti-BM28 antibody inhibits the onset of subsequent DNA synthesis as tested by the incorporation of bromodeoxyuridine. Microinjection during the S phase had no effect on DNA synthesis, but inhibits cell division. The data suggest that the nuclear protein BM28 is required for two events of the cell cycle, for the onset of DNA replication and for cell division.


Subject(s)
Cell Cycle Proteins , Cell Division , Nuclear Proteins/metabolism , S Phase , 3T3 Cells , Amino Acid Sequence , Animals , Antibodies/pharmacology , Cell Line , Cloning, Molecular , Consensus Sequence , DNA/biosynthesis , DNA Replication , HeLa Cells , Humans , Kinetics , Mice , Minichromosome Maintenance Complex Component 2 , Molecular Sequence Data , Nuclear Proteins/chemistry , Nuclear Proteins/genetics , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Schizosaccharomyces/genetics , Schizosaccharomyces/metabolism , Sequence Homology, Amino Acid
7.
Exp Cell Res ; 199(2): 398-401, 1992 Apr.
Article in English | MEDLINE | ID: mdl-1544381

ABSTRACT

Mitotin is a nuclear protein detectable in all proliferating cells investigated so far, including human and plant cells. In interphase cells the protein is localized mainly in the nucleoplasm. In G2/M phase it displays a characteristic redistribution and a marked increase which initiated the name mitotin. This study presents the precise localization of mitotin in cytoplasmic structures in two cell types, the potoroo rat kangaroo PtK2 cell and the human lung cancer EPLC 65 cell. In addition to its nuclear localization the antigen is detectable in centrosomes, in the poles of the mitotic spindle, and along spindle fibers. During the last mitotic stages, cytokinesis and reconstitution of nuclei, mitotin displays a rapid decrease and another redistribution. A significant amount of the antigen is retained in the bridge connecting the dividing cells, the midbody.


Subject(s)
Nuclear Proteins/metabolism , Spindle Apparatus/metabolism , Animals , Cell Cycle , Cell Line , Fluorescent Antibody Technique , Humans , Interphase , Lung Neoplasms , Macropodidae , Microscopy, Fluorescence , Tumor Cells, Cultured
8.
Cell Biol Int Rep ; 15(8): 661-5, 1991 Aug.
Article in English | MEDLINE | ID: mdl-1742800

ABSTRACT

The work presents the results of the immunoelectron microscopical localization of mitotin in different phases of the cell cycle. The distribution of the protein was studied using its specific monoclonal antibody and immunogold labeling in synchronized WISH cells. In S phase the antigen was found in the nucleoplasm usually over the interchromatin granules. In G2 phase the amount of mitotin increases and it can be found also in the nucleolus. In mitosis the immunogold granules are always out of the condensed chromosomes.


Subject(s)
Cell Nucleus/ultrastructure , Nuclear Proteins/ultrastructure , Antibodies, Monoclonal , Cell Cycle , Cell Nucleolus/chemistry , Cell Nucleolus/ultrastructure , Cell Nucleus/chemistry , Cells, Cultured , Chromatin/chemistry , Chromatin/ultrastructure , G2 Phase , Humans , Immunohistochemistry , Microscopy, Immunoelectron/methods , Mitosis , Nuclear Proteins/analysis , S Phase
9.
Biol Cell ; 72(1-2): 47-50, 1991.
Article in English | MEDLINE | ID: mdl-1756311

ABSTRACT

Mitotin is a 125 kDa/pI 6.5 nuclear protein specific for proliferating cells and markedly increased prior to and during mitosis. This study presents evidence for the expression of this protein during dimethylsulfoxide (DMSO) induced differentiation of human promyelocytic leukemia HL 60 cells. The expression had been followed at two levels: as antigen, using a specific antimitotin monoclonal antibody and as mRNA, using a specific cDNA probe. The results from the immunofluorescent study show a gradual disappearance of mitotin in differentiating HL 60 cells starting from the fourth day after DMSO induction. On the other hand, the changes in the expression of mitotin mRNA were much more dramatic. This mRNA is expressed at a high level during the first three days of differentiation but shows a striking decrease after the fourth day. This correlates with the rapid changes in the number of blast cells in the differentiating HL 60 cell population. Therefore, the expression of mitotin mRNA can serve as a marker for the changes accompanying the termination of cell proliferation in differentiating cells.


Subject(s)
Nuclear Proteins/isolation & purification , RNA, Neoplasm/isolation & purification , Antibodies, Monoclonal , Antigens, Neoplasm/metabolism , Blotting, Northern , Cell Differentiation , Cell Division , DNA Probes , Dimethyl Sulfoxide , Fluorescent Antibody Technique , Humans , Nuclear Proteins/genetics , RNA, Messenger/analysis , Tumor Cells, Cultured
10.
J Cell Sci ; 95 ( Pt 1): 59-64, 1990 Jan.
Article in English | MEDLINE | ID: mdl-2190998

ABSTRACT

The preparation of mammalian cells for entry into mitosis is related to a cascade of G2 phase phosphorylations of several nuclear proteins driven by mitosis-specific protein kinases. Using a monoclonal antibody we have identified previously in mammalian cells a 125K/pI6.5 protein, associated with the nuclear matrix, and markedly increased in mitotic cells, which was named 'mitotin'. Here, we show by short-term [35S]methionine labeling of cell cycle synchronized cells that this protein is synthesized at comparable rates throughout interphase. However, upon cycloheximide block of protein synthesis mitotin labeled during S phase is rapidly degraded, while the degradation of mitotin labeled during late G2 phase is abolished, resulting in its net and marked increase. The accumulation of mitotin in premitotic and mitotic cells is related to its phosphorylation and the metabolic stability of its two phosphorylated forms. The metabolic stabilization and accumulation of a nuclear matrix protein upon phosphorylation suggests the operation of a novel mechanism among the complex events preparing the cell for mitosis.


Subject(s)
Mitosis/physiology , Nuclear Matrix/physiology , Nuclear Proteins/metabolism , Cells, Cultured , Fluorescent Antibody Technique , Humans , Interphase/physiology , Phosphorylation
11.
Cell Biol Int Rep ; 11(3): 181-7, 1987 Mar.
Article in English | MEDLINE | ID: mdl-3495352

ABSTRACT

An anti-nucleolar monoclonal antibody reacting with human B-lymphoblastoid cells but not with normal peripheral blood lymphocytes has been isolated. The antibody recognized in Namalwa cells an antigen with molecular mass 41 kDa and pI 5.6, different from all previously described nucleolar antigens. Inhibition of rRNA transcription with Actinomycin D caused redistribution of the 41/5.6 antigen, but even at long term drug action it remains associated with the nucleolar remnants.


Subject(s)
Antigens, Neoplasm/analysis , B-Lymphocytes/ultrastructure , Cell Nucleolus/ultrastructure , Animals , Antibodies, Monoclonal , Antigen-Antibody Complex , Cell Line , Cell Nucleolus/analysis , Dactinomycin/pharmacology , Electrophoresis, Polyacrylamide Gel , Humans , Mice , Mice, Inbred BALB C , Molecular Weight , RNA, Ribosomal/genetics , Transcription, Genetic/drug effects
12.
Biol Cell ; 60(1): 1-8, 1987.
Article in English | MEDLINE | ID: mdl-2960400

ABSTRACT

A monoclonal antibody of the IgG2a subclass was isolated from the supernate of a hybridoma line obtained with splenocytes from a mouse immunized with a crude nucleolar fraction of human Namalwa cells. This antibody identifies a single nuclear polypeptide antigen characterized by: (a) presence in proliferating human cell lines and phytohemagglutinin-stimulated lymphocytes, but absence in resting lymphocytes; (b) appearance in stimulated lymphocytes in parallel with the onset of DNA synthesis; (c) a speckled distribution in the nucleoplasm; (d) tight association with nuclear matrix structures identified by both biochemical and in situ extraction and enzyme treatment procedures; (e) mol wt of 125 kDa and pI 6.5 as determined by immunoprecipitation or immunoblotting of nuclear or nuclear matrix proteins fractionated by gel electrophoresis. The above characteristics identify the p125/6.5 nuclear matrix protein recognized by the isolated monoclonal antibody as belonging to the class of proliferating cell nuclear antigens.


Subject(s)
Autoantigens/analysis , Cell Nucleus/immunology , Nuclear Proteins/analysis , Antibodies, Monoclonal , Antigens, Nuclear , Cell Division , Cell Line , Cell Nucleus/ultrastructure , Fluorescent Antibody Technique , Humans , Immunoglobulin G , Peptides/analysis , Peptides/immunology
SELECTION OF CITATIONS
SEARCH DETAIL
...