Molecular detection and genotyping of Enterocytozoon bieneusi in family pet dogs obtained from different routes in Japan.

The present study determined the prevalence of Enterocytozoon (E.) bieneusi infection and genotyped the isolates in family pet dogs obtained from three different routes in Japan. Fresh fecal samples were collected from 597 family pet dogs. A nested polymerase chain reaction (PCR) assay targeting the internal transcribed spacer (ITS) region of ribosomal DNA was employed for detection of E. bieneusi, and the obtained positive PCR amplicons were sequenced for genotyping. The overall prevalence of E. bieneusi in family pet dogs was 4.4%. Significant differences in prevalence were found among the categories of age (<1 year: 8.3% vs. ≥1 year: 3.4%), obtained route (from private owner: 3.2% vs. from pet shop: 3.9% vs. from breeding kennel: 14.3%), and living regions (ranged 0-10.3%). However, no significant differences were observed in the prevalence of living condition (indoor: 4.6% vs. outdoor: 2.0%), and fecal condition (formed: 4.5% vs. soft: 4.7% vs. diarrhea: 0%). Of the 26 sequenced samples, all were identified as genotype PtEb IX, which belongs to the dog-specific genotype. In conclusion, although E. bieneusi infections are relatively common in family pet dogs in Japan, the risk of zoonotic transmission from dogs to humans is likely to be low.

Molecular Prevalence of Cryptosporidium spp. among Companion Birds Kept in Pet Shops in Japan.

Cryptosporidium is the most common protozoan that can infect a wide range of animals, including mammals and birds. Avian Cryptosporidium spp. can cause enteric and respiratory diseases which can be fatal in birds and some species are zoonotic. Companion birds have the potential as reservoir due to their close contact with humans. Pet shops are the major source of companion birds. However, few reports are available regarding Cryptosporidium spp. infection among companion birds kept in pet shops. The present study reports the prevalence and molecular characteristics of Cryptosporidium spp. among companion birds kept in pet shops in Japan. A total of 265 fresh fecal samples were obtained from birds kept in 4 pet shops; these birds belonged to 41 species in 3 bird orders. A nested polymerase chain reaction (PCR) assay targeting the small subunit rRNA gene was employed for the detection of Cryptosporidium spp. A total of 24 samples (9.1%) were positive, and Cryptosporidium spp. were detected from all pet shops. The prevalence of Cryptosporidium spp. in each of the bird orders was 6.5% (10/153) in Psittaciformes, 14.4% (13/90) in Passeriformes, and 4.5% (1/22) in Galliformes. Based on sequence analysis, 13 (54.2%) isolates were classified to C. galli, 8 (33.3%) were avian genotype III, and the remaining 3 (12.5%) were C. baileyi. No infection with zoonotic C. meleagridis and no coinfection with multiple Cryptosporidium spp. and/or genotypes were observed. The zoonotic potential of Cryptosporidium spp. infecting companion birds kept in pet shops in Japan is likely to be low.