ABSTRACT
BACKGROUND: Helicobacter pylori infection is associated with peptic ulcer disease and gastric cancer. The aim of this work was to describe, to compare H. pylori antigenic profiles, and to characterize the antibody response against Colombian strains in gastric cancer and peptic ulcer patients. MATERIALS AND METHODS: Liquid culture supernatants were used to determine the antigenic profiles of 35 H. pylori strains by immunoblotting using a pool of positive sera. Characterization of strains included the evaluation of cytotoxic and vacuolating activities. The serologic antibody profiles of 124 patients (54 duodenal ulcer and 70 gastric cancer) were analyzed against two native strains (Hpu24, Hpc29) and NCTC11638. Antibodies to specific antigenic bands in each strain were related with presentation. RESULTS: Differences among antigenic profiles were observed between native isolates with each serum recognizing a wide range of antigens (30-120 Kd). A 68 Kd band in Hpu24 strain was recognized by 50% of sera from peptic ulcer patients but not by gastric cancer sera (p =.000). The immune profiles differed according to the strain used (i.e. a given sera did not recognize the same bands in different strains). Detection of H. pylori in gastric mucosa was associated with the presence of antibodies against low molecular weight antigenic bands. CONCLUSIONS: The heterogeneity in the antibody response to H. pylori and the prevalence specific anti-H. pylori antibodies in a specific disease depend on the strain used as antigen. The results support the hypothesis that there may be a differential antibody response to carcinogenic and ulcerogenic strains and suggest that there are antigenic bands that could be useful as markers of disease.
Subject(s)
Helicobacter Infections/complications , Peptic Ulcer/microbiology , Stomach Neoplasms/microbiology , Adult , Antibodies, Bacterial/immunology , Antigens, Bacterial/immunology , Colombia , HeLa Cells , Helicobacter Infections/drug therapy , Helicobacter Infections/immunology , Helicobacter pylori/immunology , Helicobacter pylori/pathogenicity , Humans , Middle Aged , Peptic Ulcer/drug therapy , Peptic Ulcer/immunology , Peptic Ulcer/pathology , Species Specificity , Stomach Neoplasms/drug therapy , Stomach Neoplasms/immunology , Stomach Neoplasms/pathology , VacuolesABSTRACT
El trastorno del estrés postraumático (TSPT) es una entidad nosológica clasificada desde 1980 por el DSM III, asociado a eventos traumáticos extraordinarios para el paciente y se presenta al cabo de mínimo 6 horas. El estudio abarca una muestra de 31 pacientes, seleccionados por haber sufrido herida por arma de fuego o cortopunzante en tórax y/o abdomen, de los cuales 16 cumplieron los criterios para el DSM III-R para el TSPT. Se encontró que el criterio que mas se cumplía en la población estudiada era el criterio referente a revivir el evento (26 pacientes) (criterio B). El item que más cumplieron los 31 pacientes fué el referido a alteración psicológica ante situaciones similares al evento (criterio B4) correspondiendo al 100 por ciento de población estudiada; de los pacientes que desarrollaron el TSPT todos presentaron hipervigilancia con una significancia estadística (p<0.0001). El estudio permite mostrar que el TSPT si se presenta en nuestra población además permite sugerir que los pacientes con las características establecidas para el presente estudio ameritan un control psicológico a mediano plazo como mínimo
