ABSTRACT
Daylight distribution is an essential performance parameter for building facades that aim to maximize user comfort while maintaining energy efficiency. This study investigates the feasibility of using 3D-printed thermoplastic to improve daylight distribution and transmission. To identify how geometry influences light distribution and transmission, 12 samples with various patterns were robotically fabricated. In a physical simulation of spring, summer, and winter, a robotic arm was used to direct light onto the samples in both the vertical and horizontal print pattern directions. In addition, three samples of conventional facade materials, including a polycarbonate panel, a polycarbonate sheet, and a single sheet of glass, were compared with the 3D-printed samples. All samples were examined and compared using high dynamic range imaging to qualitatively characterize luminance. The data analysis demonstrated that 3D-printed geometry can successfully generate customizable diffusive light distribution based on the needs of the user. Furthermore, the results showed that the vertical pattern direction had higher light transmission values than the horizontal pattern direction.
ABSTRACT
The aim of this study was to evaluate the susceptibility of 197 isolates of Lactobacillus paracasei, isolated from Italian fermented products coming from different geographical areas, to tetracycline and erythromycin, two antimicrobials widely used in clinical and animal therapy. Isolation media were supplemented with antibiotics according to the microbiological breakpoints (BPs) defined by European Food Safety Authority (EFSA). Isolates were identified at the species level and were typed by rep-PCR using the (GTG)(5) primer. A total of 121 genotypically different strains were detected and their phenotypic antimicrobial resistance to tetracycline and erythromycin was determined as the minimum inhibitory concentration (MIC) using the broth microdilution method. The presence of the genes ermB, ermC and tetL, tetM, tetS, tetW, in the phenotypically resistant isolates was investigated by PCR. Tetracycline induction of tetM expression on representative resistant strains, grown in medium either lacking or containing the antibiotic, was also analyzed by RT-PCR. Among the 121 tested strains, 77.7% were susceptible to tetracycline (MIC