ABSTRACT
OBJECTIVE: This cross-sectional study assesses the profile and antibiotic susceptibility of aerobic bacterial pathogens associated with diabetic foot ulcers (DFUs). MATERIALS AND METHODS: Two swab samples from 140 DFUs with various Wagner grades were processed for identification using routine culture methods and antimicrobial susceptibility by Kirby-Bauer disc diffusion method. RESULTS: A total of 125 (89.29%) samples were found to be positive for bacteria on culture. A higher incidence of positive culture (94.32%) was found in individuals with a blood sugar level > 200 mg/dL. The highest number of culture-positive cases was observed in Wagner grade 2 DFUs (45%). Overall infection was monomicrobial in 83.20% (104) and polymicrobial in 16.80% (21) of samples. Staphylococcus aureus (21.09%) and Pseudomonas aeruginosa (19.05%) were the most common isolates. Linezolid (100%) and imipenem (75.70%) were the most effective antimicrobial agents against gram-positive and gram-negative isolates, respectively. CONCLUSIONS: The results show an overall increase in bacterial resistance to antimicrobial agents and emphasize the importance of an antimicrobial susceptibility pattern in the selection of appropriate antibiotic(s) to institute the rational antibiotic therapy.
Subject(s)
Anti-Bacterial Agents/pharmacology , Diabetic Foot/drug therapy , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Adult , Anti-Bacterial Agents/therapeutic use , Cross-Sectional Studies , Diabetic Foot/microbiology , Drug Resistance, Bacterial , Female , Gram-Negative Bacteria/isolation & purification , Gram-Positive Bacteria/isolation & purification , Humans , Male , Microbial Sensitivity Tests , Middle Aged , Pseudomonas aeruginosa/drug effects , Pseudomonas aeruginosa/isolation & purification , Sensitivity and Specificity , Staphylococcus aureus/drug effects , Staphylococcus aureus/isolation & purificationABSTRACT
In the present study an attempt was made to find out bacteria and fungi causing corneal ulcers. Sixty cases of corneal ulcer were studied. Corneal scrapings from patient of corneal ulcer were collected and processed for isolation of bacterial and fungal agents. The commonest age group affected was 21-30 yrs. Incidence of corneal ulcer was more in males 39 (65%) as compared to females 21 (35%). Trauma was the commonest predisposing factor. Out of sixty cases of corneal ulcer positive cultures were obtained in 23 (38.33%). Bacteria were isolated in 10 (16.66%) fungi in 12 (20%) and mixed culture in 1 (1.66%) of the patients. Among the bacterial isolates commonest was Staphylococcus aureus 6 (10%). Among fungal isolates Fusarium species was the commonest isolate 4 (6.66).
