Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add more filters










Database
Language
Publication year range
1.
Am J Trop Med Hyg ; 95(2): 315-21, 2016 08 03.
Article in English | MEDLINE | ID: mdl-27185768

ABSTRACT

During an active surveillance study in school children in Medellin, we assessed the performance of two diagnostic strategies for dengue virus. A total of 41 patients with suspected dengue acute infection were evaluated. Diagnostic strategies consisted of one combining Panbio(®) Dengue virus IgM and IgG Capture ELISAs (enzyme-linked immunosorbent assays) with reverse transcriptase polymerase chain reaction (RT-PCR) and another using a commercial rapid SD Bioline Dengue Duo (IgG/IgM + NS1 Ag) test. These two strategies were compared with the enzyme-linked immunospot microneutralization test (ELISPOT-MNT). The sensitivity and specificity were 53.9% and 80.0% for the combination of Panbio(®) ELISAs and RT-PCR tests, and 30.8% and 73.3% for the SD Bioline Duo test, respectively. ELISPOT-MNT detected 16.4% additional cases and revealed the presence of neutralizing antibodies in all the acute samples, evidencing that they were all secondary infections. In contrast, Panbio(®) and SD Dengue Duo rapid tests only classified 23.0% and 26.9% of the cases as secondary dengue infections, respectively. Cohen's kappa coefficient and McNemar's association tests demonstrated a significant disagreement between the two diagnostic strategies and ELISPOT-MNT. Overall, these results evidence the relatively poor performances of commercial assays for the diagnosis of acute and secondary dengue infections, compared with ELISPOT-MNT, and raise concern about the accuracy of these assays for the diagnostic of dengue in endemic areas.


Subject(s)
Antibodies, Viral/blood , Dengue Virus/isolation & purification , Dengue/diagnosis , Dengue/epidemiology , Epidemics , Acute Disease , Adolescent , Child , Colombia/epidemiology , Dengue/immunology , Dengue/virology , Dengue Virus/genetics , Dengue Virus/immunology , Enzyme-Linked Immunosorbent Assay/standards , Enzyme-Linked Immunosorbent Assay/statistics & numerical data , Enzyme-Linked Immunospot Assay/standards , Enzyme-Linked Immunospot Assay/statistics & numerical data , Female , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Male , Reverse Transcriptase Polymerase Chain Reaction/standards , Reverse Transcriptase Polymerase Chain Reaction/statistics & numerical data , Schools , Sensitivity and Specificity
2.
Am J Trop Med Hyg ; 87(3): 565-72, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22802436

ABSTRACT

Serum samples from a total of 71 healthy captive birds belonging to 18 species were collected in July of 2008 in Medellin (Colombia) and tested for flaviviruses. Eighteen of 29 samples from American Flamingoes (Phoenicopterus ruber) were positive for West Nile virus (WNV) by reverse transcription-polymerase chain reaction. Selected positive samples were serially passaged and WNV was confirmed by immunofluorescence. Two isolates (524/08, 9835/08) were characterized in vitro and in vivo. Sequence analysis revealed WNV with 16 nucleotide substitutions resulting in six amino acid changes when compared with the NY99 strain. Colombian (COL) viruses were more closely related to Louisiana isolates from 2001. When compared with attenuated strains isolated from Texas, COL isolates differed in their plaque size and temperature sensitivity phenotype. The COL viruses were pathogenic in embryonated chicken eggs and Balb/c mice.


Subject(s)
Bird Diseases/virology , Birds/virology , RNA, Viral/isolation & purification , West Nile Fever/veterinary , West Nile virus/isolation & purification , Animals , Chickens/virology , Colombia , Louisiana , Mice , Mice, Inbred BALB C , Phenotype , RNA, Viral/genetics , Reverse Transcriptase Polymerase Chain Reaction , Texas , West Nile Fever/genetics , West Nile Fever/virology , West Nile virus/classification , West Nile virus/genetics
SELECTION OF CITATIONS
SEARCH DETAIL
...