Influence of dentinal polyelectrolytes on wet demineralized dentin, a bonding substrate.

The objective of this study was to show the influence of dissolved dentinal polyelectrolytes on the characteristics of dentin (bonding substrate) demineralized by citric acid in the absence or presence of ferric chloride. The demineralizing agent was an aqueous mixture of 0, 1, 3, or 10% ferric chloride in 10% citric acid (10-0, 10-1, 10-3, 10-10, respectively). The hypothesis was that the concentration of dissolved dentinal noncollagenous substances, mainly polyelectrolytes soluble in water, must be decreased by their aggregation with ferric ions, which changes the characteristics of demineralized dentin, the rates of demineralization, and dehydration. Cervical bovine dentin was prepared in 3 x 2 x 2-mm blocks, each weighing 20.0 +/- 0.5 mg. The rate of demineralization was investigated by measuring the weight loss resulting from demineralization by immersion in 10 mL of conditioner at 2-h intervals. The dehydration rate of wet demineralized dentin was determined using two methods: (1) weight loss in a desiccator under 263 Pa pressure and (2) differential scanning calorimetry (DSC). Twenty, 12, 8, and 4 h were required to complete demineralization of the blocks with the 10-0, 10-1, 10-3, and 10-10 solutions, respectively. The 10-10 wet demineralized dentin showed the highest rate of dehydration, followed in descending order by the 10-3, 10-1, and 10-0 specimens. Ferric chloride in dentin conditioners provided both a higher rate of dentin demineralization and a higher dehydration rate of wet demineralized dentin. These results suggest that in the presence of ferric chloride, a decreasing amount of dissolved polyelectrolytes aggregated with ferric ions in the substrates may increase the permeability of dentin to water and citric acid. Improvement of monomer permeability is essential to the preparation of good hybridized dentin, providing a more stable and reliable bonding and also protecting the dentin and pulp from infection. A further study of bonding substrates is required in order to understand the role of hybridized dentin in improved dental treatment.

Effect of seating force, margin design, and cement on marginal seal and retention of complete metal crowns.

PURPOSE: The purpose of this study was to determine the marginal discrepancy and retention of silver-palladium crowns cemented with zinc phosphate (Phosphacap) and glass-ionomer cement (Fuji Cap 1) using different seating forces on preparations with various margin designs. MATERIALS AND METHODS: Crown preparations with three finish lines--chamfer, shoulder, and shoulder with a 45-degree bevel--were sequentially prepared on a dentoform premolar. A metal die for each of the three finish lines was constructed. Complete metal crowns were fabricated for each metal die using a silver-palladium alloy. Three different seating forces--25, 100, and 300 N--were used to load the crowns until initial set of the cement. The marginal discrepancy was calculated by measuring the change in crown height before and after cementation using a digimatic indicator. Retention was determined by measuring the tensile strength using the Lloyd universal testing machine. RESULTS: The higher seating forces produced better crown seating but had no significant effect on crown retention. The shoulder and shoulder with bevel finish lines provided better crown retention than the chamfer. Glass-ionomer cement provided greater crown retention than zinc phosphate cement. No significant correlation between marginal seating and crown retention was revealed using a Pearson analysis. CONCLUSION: Marginal seal was not influenced by either margin design or type of luting cement, but was improved with higher seating force. Crown retention was affected by the margin finish line and the luting agent.

Effect of dentin conditioners on wet bonding of 4-META/MMA-TBB resin.

PURPOSE: By altering either ferric chloride concentration in 10% citric acid (1% ferric chloride = 10-1; 5% ferric chloride = 10-5; 10% ferric chloride = 10-10) or conditioning periods with an aqueous mixture of 1% citric acid and 1% ferric chloride (1-1), the influence of dentin substrate on bond strength and hybridized dentin in wet bonding of 4-META/MMA-TBB resin was examined. MATERIALS AND METHODS: Dentin surfaces of fresh bovine incisors were conditioned either with 10-1, 10-5, or 10-10 mixtures for 10 s (10-1-10s, 10-5-10s, 10-10-10s groups) or with a 1-1 mixture for 5, 10, 30 or 60 s (1-1-5s, 1-1-10s, 1-1-30s, 1-1-60s groups). Rinsed, demineralized dentin samples were kept wet, primed with 5% 4-META in acetone for 60 s, and bonded with 4-META/MMA-TBB resin. Bonded specimens were trimmed to a mini-dumbbell shape for tensile testing. The cross sections of bonded specimens were modified with HCl and NaOCl in order to assess the hybrid layer. The fractured surfaces of specimens and the hybridized dentin were investigated with SEM. RESULTS: No significant difference (p > 0.01) in tensile strength was identified between 10-1-10s and 10-5-10s groups (30 MPa), 10-10-10s and 1-1-5s groups (15 MPa), and the three groups conditioned by 1-1-10s, -30s and -60s (40 MPa). The thickness of the hybrid layer increased with increasing either ferric chloride or conditioning periods. CONCLUSION: The concentration of ferric chloride in 10% citric acid for wet bonding must be less than 5% in order to provide a reliable bond. When applied from 10 to 60 s, the 1-1 conditioner provided hybridized dentin with reliable tensile bond strength. The thickness of the hybrid layer did not influence the tensile bond strength.