Subject(s)
Bone and Bones/diagnostic imaging , Carcinoma, Non-Small-Cell Lung/diagnostic imaging , Diaphragm/diagnostic imaging , Kidney/diagnostic imaging , Lung Neoplasms/diagnostic imaging , Female , Humans , Middle Aged , Pleural Diseases/diagnostic imaging , Pleural Effusion/diagnostic imaging , Radionuclide Imaging , Tomography, X-Ray ComputedABSTRACT
A case of umbilical metastasis from prostatic carcinoma is presented. This diagnosis was supported by positive prostate specific antigen immunoperoxidase staining. Umbilical metastasis is usually associated with other types of tumors and has a grave prognosis.
Subject(s)
Adenocarcinoma/secondary , Prostatic Neoplasms/pathology , Skin Neoplasms/secondary , Umbilicus , Aged , Humans , MaleABSTRACT
We compared three radioligands for use in a cytoplasmic estrogen-receptor assay, using pooled cytosol from human breast adenocarcinomas. The estrogen receptor content was determined in vitro by a dextran-coated charcoal method involving a 4-h incubation with and without diethylstilbestrol. Tritiated moxestrol failed to come to equilibrium in 4 h, thereby preventing the use of conventional one-component Scatchard analysis. The use of 125I-labeled estradiol resulted in a higher estimate of estrogen-receptor concentration than that obtained with use of tritiated estradiol. This overestimation was not corrected by Scatchard, double-reciprocal, or Woolf plots, or by two different methods of data analysis: least squared and "robust." An underestimation of the specific activity of iodoestradiol with respect to that of tritiated estradiol and an unrecognized second component of nonreceptor binding could explain this disparity.
Subject(s)
Breast Neoplasms/analysis , Radioligand Assay/methods , Receptors, Estrogen/analysis , Cytoplasm/analysis , Estradiol/metabolism , Female , Humans , Iodine Radioisotopes , TritiumABSTRACT
A case of leiomyomatosis peritonealis disseminata studied by light and transmission electron microscopy is reported. The lesion, from a pregnant woman, was found to contain predominantly myofibroblasts and decidualized cells in a rich collagen stroma, while relatively few leiomyocytes and fibroblasts were observed. The development and fate of this entity are discussed in view of the present findings and those previously reported.
Subject(s)
Decidua/ultrastructure , Fibroblasts/ultrastructure , Omentum/ultrastructure , Peritoneal Diseases/pathology , Adult , Collagen , Cytoplasm/ultrastructure , Female , Humans , Muscle, Smooth/ultrastructure , Organoids/ultrastructure , PregnancyABSTRACT
A technique for using 2-M urea as a hemolyzing agent not only for fresh smears but also for previously stained smears is described. It is simple, inexpensive, rapid acting and easily prepared.
