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1.
Braz. dent. j ; 16(3): 192-196, set. -dez. 2005. ilus, tab
Article in English | LILACS | ID: lil-419841

ABSTRACT

A atividade antimicrobiana de extratos brutos de folhas de Arctium lappa, bem como de suas fases, foi avaliada in vitro. Os microrganismos Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis e Candida albicans, comuns na cavidade bucal, especificamente em infecções endodônticas, foram utilizados. O método de difusão em Agar permitiu a detecção da fase hexânica como inibitória do crescimento microbiano. Ensaios de bioautografia identificaram substâncias antimicrobianas presentes no extrato. Os resultados demonstraram a presença, na fase hexânica bruta e em suas sub-frações, de constituintes que têm Rf (fatores de retenção) em três zonas distintas, sugerindo a presença de ativos com estruturas químicas de diferentes polaridades, que exibiram especificidade contra os microrganismos alvos. Conclui-se que os constituintes de Arctium lappa apresentam um grande potencial de inibição microbiana contra os microrganismos endodônticos estudados.


Subject(s)
Dental Pulp , Focal Infection, Dental , In Vitro Techniques , Lappa arctium , Infection Control, Dental , Mouth , Pulpitis
2.
Braz Dent J ; 16(3): 192-6, 2005.
Article in English | MEDLINE | ID: mdl-16429183

ABSTRACT

This study evaluated in vitro the antimicrobial activity of rough extracts from leaves of Arctium lappa and their phases. The following microorganisms, commonly found in the oral cavity, specifically in endodontic infections, were used: Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis and Candida albicans. The agar-diffusion method allowed detection of the hexanic phase as an inhibitor of microbial growth. Bioautographic assays identified antimicrobial substances in the extract. The results showed the existence, in the rough hexanic phase and in its fractions, of constituents that have retention factors (Rf) in three distinct zones, thereby suggesting the presence of active constituents with chemical structures of different polarities that exhibited specificity against the target microorganisms. It may be concluded that the Arctium lappa constituents exhibited a great microbial inhibition potential against the tested endodontic pathogens.


Subject(s)
Anti-Bacterial Agents/pharmacology , Arctium , Bacteria/drug effects , Dental Pulp Diseases/microbiology , Plant Extracts/pharmacology , Plant Leaves , Acetates , Bacillus subtilis/drug effects , Candida albicans/drug effects , Enterococcus faecalis/drug effects , Hexanes , Humans , Materials Testing , Methanol , Microbial Sensitivity Tests , Plant Extracts/analysis , Pseudomonas aeruginosa/drug effects , Solvents , Staphylococcus aureus/drug effects
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