Intestinal bacteria diversity of suckermouth catfish (Pterygoplichthys pardalis) in the Cd, Hg, and Pb contaminated Ciliwung River, Indonesia.

The contamination of aquatic environments with heavy metals poses a serious threat to fish, potentially leading to diseases or even death. Therefore, there is an urgent need for studies to investigate the adaptability of fish in heavy metal-contaminated environments. Several studies have explored the adaptability of suckermouth catfish (P. pardalis) to survive in the contaminated Ciliwung River. The findings obtained showed that the presence of intestinal bacteria helped these fish overcome the heavy metals in their intestines, thereby enabling the fish to survive. Analysis using the Next Generation Sequencing (NGS) technology has succeeded in identifying diversity of these bacteria in P. pardalis living in the Ciliwung River, which contaminated with Cd (0.3-1.6 ppm in the water & 0.9-1.6 ppm in the sediment), Hg (0.6-2 ppm in the water & 0.6-1.8 ppm in the sediment), and Pb (59.9-73.8 ppm in the water & 26.1-58.6 ppm in the sediment). Diversity index of intestinal bacteria in P. pardalis was relatively high, but it had a negative correlation with the presence of these contaminants. Actinobacteria, Firmicutes, and Proteobacteria were abundant in the intestines of P. pardalis from the upstream to downstream of the river, with an overall abundance range of 15-48%. Furthermore, Mycobacterium along with 6 other genera were identified as core intestinal bacteria. The presence of these bacterial communities in all the samples affected their survival in heavy metals-contaminated rivers. The fish's adaptability to live in this harsh environment indicated that it has the potential to be utilized as a bioremediator of heavy metals in river sediments.

Microalgae Diversity in Interim Wet Storage of Spent Nuclear Fuel in Serpong, Indonesia.

The water quality in the interim wet storage of spent fuel (ISSF) needs to be monitored due to its function as a radiation shield. Water in ISSF pools must be free from microorganisms such as microalgae that live in a radioactive environment. Moreover, particular microalgae are capable of causing corrosion to stainless steel, which is a component of ISSF. Therefore, this study aims to determine the diversity of microalgae in the ISSF and those living in a radioactive environment, which cause corrosion. The microalgae were detected using the diversity and Palmer indices. The sampling of microalgae water was carried out by vertical filtration method at eight sites of ISSF. The results show that the diversity of microalgae (H') was low due to radiation exposure in pool water, hence, only specific species can survive. The evenness (J') of the microalgae was low, causing a high dominance index (C) value. Furthermore, the dominating species, namely, Chlorella sp. (Chlorophyceae), needs to be monitored because it has gamma radioresistance capabilities and can cause the corrosion of stainless steel.

Characteristics of tropical freshwater microalgae Micractinium conductrix, Monoraphidium sp. and Choricystis parasitica, and their potency as biodiesel feedstock.

The depletion of fossil fuel reserves requires advance anticipation through the search for alternative energy from renewable natural resources. Microalgae have been known as potential organisms for biodiesel feedstock. However, in order to be developed on a large scale, microalgae must have superior traits so that further development becomes more comfortable and cheaper. Tropical lakes are a source of superior microalgae adapted to moderate conditions which can later save operational costs in large-scale production. Situ Gintung and Situ Pamulang are the two largest lakes in South Tangerang, Indonesia and are potential sources of microalgae. Four microalgae isolates from both lakes have been identified, and their potential has been examined. Within an observation period of 18 days, they showed similar growth patterns reaching more than 108 cells mL-1 on day 14 and were able to resist increasing pH. The microalgae were identified through morphological observations and the sequencing of 23S rRNA genes with phylogenetic analysis. Each strain has a positive quality. Isolate G4-3, which was identified as Micractinium conductrix, and P5-4, which was identified as Monoraphidium sp., yielded biomass that exceeded 1.2 g L-1 with lipid content exceeding 60%. Likewise, G4-9, which was also identified as Micractinium conductrix, and P2-15, which was identified as Choricystis parasitica, have lipid content which accounted for 89.10% and 57.48%, respectively; although their biomass was lower. The percentage of fatty acid methyl esters of the four microalgae achieved >60-80%; thus, meeting the standard of biodiesel properties. Therefore, the microalgae isolates have great potential for being developed as biodiesel feedstock.