Integrated single- and two-photon light sheet microscopy using accelerating beams.

We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.

Endlessly single-mode photonic crystal fiber as a high resolution probe.

We sample ultra-broadband light, focused onto a diffraction-limited spot, to an endlessly single-mode photonic crystal fiber (ESM) and detect both the field amplitude and phase using a SEA TADPOLE interferometer. We resolve spatial features up to 2.5 times finer than the fiber mode size while sampling the periodic features of the bipolar oscillating field in the transverse section. The resolution enhancement is expected also in other types of single-mode fibers in intensity measurements and leads to an inexpensive method for characterizing the point-spread function of such optical fields, e.g., diffraction-limited spots from microscope objectives. In addition, we demonstrate the guidance of a high-NA light field in the fine structure of an ESM fiber mode. The results are especially valuable for devices where a fiber tip acts as an input slit and defines the spatial resolution, e.g., fiber-based interferometers, spectrometers, and sensors.

Macro-optical trapping for sample confinement in light sheet microscopy.

Light sheet microscopy is a powerful approach to construct three-dimensional images of large specimens with minimal photo-damage and photo-bleaching. To date, the specimens are usually mounted in agents such as agarose, potentially restricting the development of live samples, and also highly mobile specimens need to be anaesthetized before imaging. To overcome these problems, here we demonstrate an integrated light sheet microscope which solely uses optical forces to trap and hold the sample using a counter-propagating laser beam geometry. Specifically, tobacco plant cells and living Spirobranchus lamarcki larvae were successfully trapped and sectional images acquired. This novel approach has the potential to significantly expand the range of applications for light sheet imaging.

Realization of laterally nondispersing ultrabroadband Airy pulses.

We present the measurements of the spatiotemporal impulse response of a system creating nondispersing Airy pulses, i.e., ultrabroadband Airy beams whose main lobe size remains constant over propagation. A custom refractive element with a continuous surface profile was used to impose the cubic phase on the input beam. The impulse response of the Airy pulse generator was spatiotemporally characterized by applying a white-light spatial-spectral interferometry setup based on the SEA TADPOLE technique. The results were compared with the theoretical model and previously spatiotemporally characterized Airy pulses generated by a spatial light modulator.

Spatiotemporal characterization of ultrabroadband Airy pulses.

We present experimental results of a full spatiotemporal characterization of an optical system for ultrabroadband Airy pulse generation with a liquid-crystal-on-silicon spatial light modulator. Measurements with a few micrometer spatial and almost one-wave-cycle temporal resolution were performed using a white light spatial spectral interferometry setup based on the SEA TADPOLE ultrashort pulse characterization technique. The results were compared with the theoretical model for Airy pulse propagation.

Basic diffraction phenomena in time domain.

Using a recently developed technique (SEA TADPOLE) for easily measuring the complete spatiotemporal electric field of light pulses with micrometer spatial and femtosecond temporal resolution, we directly demonstrate the formation of theo-called boundary diffraction wave and Arago's spot after an aperture, as well as the superluminal propagation of the spot. Our spatiotemporally resolved measurements beautifully confirm the time-domain treatment of diffraction. Also they prove very useful for modern physical optics, especially in micro- and meso-optics, and also significantly aid in the understanding of diffraction phenomena in general.

Direct spatiotemporal measurements of accelerating ultrashort Bessel-type light bullets.

We measure the spatiotemporal field of ultrashort pulses with complex spatiotemporal profiles using the linear-optical, interferometric pulse-measurement technique SEA TADPOLE. Accelerating and decelerating ultrashort, localized, nonspreading Bessel-X wavepackets were generated from a approximately 27 fs duration Ti:Sapphire oscillator pulse using a combination of an axicon and a convex or concave lens. The wavefields are measured with approximately 5 microm spatial and approximately 15 fs temporal resolutions. Our experimental results are in good agreement with theoretical calculations and numerical simulations.

Measuring the spatiotemporal field of ultrashort Bessel-X pulses.

We present direct measurements of the spatiotemporal electric field of an ultrashort Bessel-X pulse generated using a conical lens (axicon). These measurements were made using the linear-optical interferometric technique SEA TADPOLE, which has micrometer spatial resolution and femtosecond temporal resolution. From our measurements, both the superluminal velocity of the Bessel pulse and the propagation invariance of the central spot are apparent. We verified our measurements with simulations.