1.
Stem Cell Res
; 67: 103038, 2023 03.
Article
in English
| MEDLINE
| ID: mdl-36746102
ABSTRACT
Fibroblasts isolated from a skin biopsy of a healthy individual were infected with Sendai virus containing the Yamanaka factors to produce transgene-free human induced pluripotent stem cells (iPSCs). CRISPR/Cas9 was used to generate an isogenic cell line carrying an inactivation of ST3GAL3, a risk gene associated with neurodevelopmental and psychiatric disorders. This ST3GAL3 null mutant (ST3GAL3-/-) iPSC line, which displays the expression of pluripotency-associated markers, the ability to differentiate into cells of the three germ layers in vitro, and a normal karyotype, is a powerful tool to investigate the impact of deficient sialylation of glycoproteins in neural development and plasticity.
Subject(s)
Induced Pluripotent Stem Cells , Humans , Induced Pluripotent Stem Cells/metabolism , Gene Editing , CRISPR-Cas Systems , Cell Differentiation , Cell Line
2.
Med Sci (Paris)
; 37(1): 100-102, 2021 01.
Article
in French
| MEDLINE
| ID: mdl-33492226