ABSTRACT
The experiments were performed on female Wistar rats that received aluminum chloride every day intraperitoneally, 4 mg Al/kg for 3 weeks. In the blood selected morphological factors and delta-aminolevulinic acid dehydratase activity (ALA-D) were investigated. In the kidney and liver ALA-D, delta-aminolevulinic acid synthase (ALA-S), and heme oxygenase activity were also determined. After aluminum chloride administration the most sensitive indicator was an increase of heme oxygenase activity in the liver and a decrease in iron levels in the serum of rats. Aluminum also increased ALA-S activity in the kidney and liver of rats. No changes of ALA-D activity in the liver, the kidney, and the blood were observed. The decreasing of hemoglobin, hematocrit, mean corpuscular hemoglobin mass, and mean corpuscular hemoglobin concentration was noted after 3 weeks of exposure.
Subject(s)
Aluminum Compounds/toxicity , Chlorides/toxicity , Hematopoiesis/drug effects , Kidney/drug effects , Liver/drug effects , 5-Aminolevulinate Synthetase/blood , 5-Aminolevulinate Synthetase/metabolism , Aluminum Chloride , Aluminum Compounds/administration & dosage , Analysis of Variance , Animals , Astringents/administration & dosage , Astringents/toxicity , Chlorides/administration & dosage , Female , Hematocrit , Heme Oxygenase (Decyclizing)/blood , Heme Oxygenase (Decyclizing)/metabolism , Hemoglobins/metabolism , Injections, Intraperitoneal , Iron/blood , Kidney/enzymology , Liver/enzymology , Porphobilinogen Synthase/blood , Porphobilinogen Synthase/metabolism , Rats , Rats, WistarABSTRACT
The aim of this work was to assess changes of morphological parameters in the blood of rats after oral (po) administration of aluminum (Al), in relation to the time and the administered dose. The experiment was performed on female Wistar rats. The animals were administered aluminum chloride (100 mg Al/kg) daily for 21 d. Morphological assays: red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT), iron serum concentration (Fe), MCH, MCHC, absolute corrected reticulocyte count (ACRC), white blood cells (WBC), and platelet count (PLT) were estimated on d 3, 7, 14, and 21, both in the control group and in intoxicated rats. After wk 1 of aluminum administration we observed a decrease of RBC, HCT, HGB and serum iron concentration in the blood of rats. The increase of the platelet count was observed earlier than changes in other parameters. Investigation has proved that the exposure of rats to aluminum administered orally results in normocytic anemia.
Subject(s)
Aluminum Compounds/toxicity , Anemia/chemically induced , Blood Cells/drug effects , Chlorides/toxicity , Iron/blood , Administration, Oral , Aluminum Chloride , Aluminum Compounds/administration & dosage , Aluminum Compounds/blood , Animals , Chlorides/administration & dosage , Chlorides/blood , Disease Models, Animal , Dose-Response Relationship, Drug , Erythrocyte Count/drug effects , Erythrocyte Indices/drug effects , Female , Hematocrit , Hemoglobins/metabolism , Leukocyte Count/drug effects , Platelet Count/drug effects , Rats , Rats, Wistar , Reticulocyte Count/drug effectsABSTRACT
The aim of the study was the estimation of heart muscle in response to air shock wave trauma. The animals were divided into four groups according to the time between trauma and the examination (3, 24, 48 and 120 hours). In all groups of animals ecg was performed and plasma level of creatinine kinase (CK), cardiac isoenzymes (CK-MB), lactic dehydrogenase (LDH) and its isoenzymes (LDH1-2) and alpha hydroxybutyrate dehydrogenase (HBDH) were determined. CK-MB activity as a percent of CK activity and rate of LDH isoenzymes activity were calculated. Morphologic estimation of heart injury based on macroscopic observation, histology and coronarography of heart specimens was performed. It was revealed that pathologic changes in ecg indicating intracardial conduction disturbances occur most intensively in first ten minutes after trauma. Plasma CPK, LDH and LDH1-2 activity increased significantly after 120 hrs following trauma. Morphologic manifestation of heart damage was multifocal injury of heart muscle.